標(biāo)簽內(nèi)參抗體
標(biāo)簽抗體
標(biāo)簽抗體，是指能夠高度特異性識(shí)別通過(guò)重組技術(shù)構(gòu)建的融合蛋白上特殊表位的抗體，可用于檢測(cè)和純化多種具有相同標(biāo)記的蛋白質(zhì)。抗原表位，又稱(chēng)抗原決定簇，是抗原分子中決定抗原特異性的特殊區(qū)域或基團(tuán)，是與抗體特異性結(jié)合的結(jié)構(gòu)或序列。隨著生物技術(shù)的發(fā)展，科研人員可以通過(guò)DNA重組技術(shù)，構(gòu)建包含目的基因以及表位標(biāo)記的融合蛋白，進(jìn)而通過(guò)特異性標(biāo)簽抗體對(duì)其鑒定與純化，以達(dá)到研究的需求。
華美生物提供6*His、GFP、c-MYC、GST、E-Tag、HA-Tag、Flag Tag、Sumo Tag等標(biāo)簽抗體，通過(guò)抗原親和純化，可與相應(yīng)的標(biāo)記蛋白特異性結(jié)合，從而鑒定和純化目標(biāo)蛋白。種類(lèi)齊全、特異性高，可保證您實(shí)驗(yàn)的順利進(jìn)行。
| Target | Applications | Target | Applications |
|---|---|---|---|
| 6*His | ELISA, WB | GFP | ELISA,WB,IP |
| c-MYC | ELISA, WB, IF, IP | GST | ELISA,WB |
| E-Tag | ELISA, WB, IP | HA-Tag | ELISA, WB, IF, FC, IP |
| Flag Tag | ELISA, WB, IP | Sumo tag | ELISA,WB |
內(nèi)參抗體
內(nèi)參即是內(nèi)部參照（Internal Control），對(duì)于哺乳動(dòng)物細(xì)胞表達(dá)來(lái)說(shuō)一般是指由管家基因編碼表達(dá)的蛋白(Housekeeping Proteins)，它們?cè)诟鹘M織和細(xì)胞中的表達(dá)相對(duì)恒定，在檢測(cè)蛋白的表達(dá)水平變化時(shí)常用它來(lái)做參照物。
華美生物提供的內(nèi)參抗體包括Beta-actin、GAPDH、PCNA、TUBAIA等，性能穩(wěn)定，質(zhì)量可靠，可長(zhǎng)期穩(wěn)定儲(chǔ)存，可在進(jìn)行Western blot實(shí)驗(yàn)時(shí)檢測(cè)目的產(chǎn)物、校正上樣誤差，也可作為空白對(duì)照，檢測(cè)蛋白轉(zhuǎn)膜是否完全、整個(gè)顯色或者發(fā)光體系是否正常。
| Target | Applications | Target | Applications |
|---|---|---|---|
| GAPDH | ELISA, WB, IHC, IP, IF, FC | TUBA1A | ELISA, WB, IHC, IF, FC, IP |
| Beta-Actin | ELISA | PCNA | ELISA |
產(chǎn)品優(yōu)勢(shì)
- 種類(lèi)齊全，特異性高
- 高靈敏度，可檢測(cè)較低表達(dá)水平蛋白
Western Blot analysis of HA-Tag Monoclonal Antibody (CSB-MA000141M0m)
Western Blot analysis of E-Tag Monoclonal Antibody (CSB-MA000151M0m)
Western Blot analysis of GAPDH Monoclonal Antibody (CSB-MA000071M0m)
- 高穩(wěn)定性，批內(nèi)差/批間差較小
- 經(jīng)過(guò)WB、FC、IP、IF、IHC等多重驗(yàn)證
HA-Tag Monoclonal Antibody (CSB-MA000141M0m)
Western Blot (WB)
Positive WB detected in: 3 different overexpression lysates with HA tagged
All lanes: HA-Tag antibody at 1:1000
Flow cytometry (FC)
Overlay histogram showing 293F transfected cells stained with CSB-MA000141M0m (red line) at 1:100.
Immunoprecipitation (IP)
Immunoprecipitating HA-Tag in 293F transfected whole cell lysate.
Lane 1: Mouse control IgG (1μg); Lane 2: CSB-MA000141M0m (5μg) + 293F transfected whole cell lysate (500μg); Lane 3: 293F transfected whole cell lysate (20μg)
Immunofluorescence (IF)
Immunofluorescence staining of 293F transfected cells with CSB-MA000141M0m at 1:100, counter-stained with DAPI.
GAPDH Monoclonal Antibody (CSB-MA000071M0m)
Western Blot (WB)
Positive WB detected in: Hela whole cell lysate, HepG2 whole cell lysate, Jurkat whole cell lysate, MCF-7 whole cell lysate
All lanes: GAPDH antibody at 1:2000
Immunoprecipitation (IP)
Immunoprecipitating GAPDH in Hela whole cell lysate. Lane 1: Mouse control IgG; Lane 2: CSB-MA000071M0m (3μl) + Hela whole cell lysate (500μg); Lane 3: Hela whole cell lysate (20μg)
Flow cytometry (FC)
Overlay histogram showing Hela cells stained with CSB-MA000141M0m (red line) at 1:200.
Immunofluorescence (IF)
Immunofluorescence staining of Hela cells with CSB-MA000071M0m at 1:220, counter-stained with DAPI.
Immunohistochemistry (IHC)
IHC image of CSB-MA000071M0m diluted at 1:100 and staining in paraffin-embedded human kidney tissue performed on a Leica BondTM system.
產(chǎn)品目錄
引用文獻(xiàn)
Myc tag Monoclonal Antibody referenced in "Borealin-nucleosome interaction secures chromosome association of the chromosomal passenger complex". The Journal of Cell Biology, 2019
His-Tag Monoclonal Antibody referenced in "When spider and snake get along: Fusion of a snake disintegrin with a spider phospholipase D to explore their synergistic effects on a tumor cell". Toxicon, 2019
Flag Tag Monoclonal Antibody referenced in "The role of the cytoplasmic tail of the influenza virus M2 protein in viral morphology and replication". University of Kent, 2018
His-Tag Monoclonal Antibody referenced in "Subfunctionalization of the Ruby2-Ruby1 gene cluster during the domestication of citrus". Nature Plants, 2018
