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中文名稱：Flag Tag鼠單克隆抗體
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貨號：CSB-MA000021M0m
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規格：￥400
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圖片：
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Western Blot
Positive WB detected in: 10ng Flag Tag fusion protein Flag Tag antibody at 1:100000, 1:200000, 1:400000, 1:800000, 1:1600000
Secondary
Goat polyclonal to mouse IgG at 1/50000 dilution
Predicted band size: 35 kDa
Observed band size: 35 kDa
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Western Blot
Positive WB detected in: Flag Tag fusion protein at 50ng, 25ng, 12.5ng, 6.25ng, 3.625ng, 1.5625ng, 0.78ng
All lanes: Flag Tag antibody at 1:1000
Secondary
Goat polyclonal to mouse IgG at 1/50000 dilution
Predicted band size: 35 kDa
Observed band size: 35 kDa
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Western Blot
Positive WB detected in: Flag Tag fusion protein 1, 2, 3, 4 at 50ng
All lanes: Flag Tag antibody at 1:1000
Secondary
Goat polyclonal to mouse IgG at 1/50000 dilution
Predicted band size: 59.6, 36, 35, 42 kDa
Observed band size: 59.6, 36, 35, 42 kDa
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Western Blot
Positive WB detected in: Flag Tag fusion protein at 10ng
All lanes: Company A, Company B, Company C, Company D, CSB-MA000021M0m antibody at 1:50000, 1:100000, 1:200000, 1:400000, 1:800000, 1:1600000, 1:3200000, 1:6400000
Secondary
Goat polyclonal to Mouse IgG at 1/50000 dilution
Predicted band size: 35 kDa
Observed band size: 35 kDa
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Western Blot
Positive WB detected in: Flag Tag fusion protein at 50ng, 25ng, 12.5ng, 6.25ng, 3.125ng, 1.5625ng, 0.78ng, 0.39ng
All lanes: Company A, Company B, Company C, Company D, CSB-MA000021M0m antibody at 1:1000
Secondary
Goat polyclonal to Mouse IgG at 1/50000 dilution
Predicted band size: 35 kDa
Observed band size: 35 kDa
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Western Blot
Positive WB detected in: 5 different recombinant proteins with Flag Tag
All lanes: Company A, Company B, Company C, Company D, CSB-MA000021M0m antibody at 1:5000
Secondary
Goat polyclonal to Mouse IgG at 1/50000 dilution
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Immunoprecipitating Flag Tag in Transfected HEK-293 cell whole cell lysate
Lane 1: Mouse control IgG instead of CSB-MA000021M0m in Transfected HEK-293 cell whole cell lysate
Lane 2: CSB-MA000021M0m (1μl) + Transfected HEK-293 cell whole cell lysate (500μg)
Lane 3: Transfected HEK-293 cell whole cell lysate (20μg)
For western blotting, the blot was detected with CSB-MA000021M0m at 1:5000, and a HRP-conjugated Protein G antibody was used as the secondary antibody at 1:2000
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Immunoprecipitating Flag Tag in 293F transfected whole cell lysate
Lane 1: Mouse control IgG instead of CSB-MA000021M0m in Flag Tag in 293F transfected whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000)
Lane 2: Company A (1μl), Company B (1μl), Company C (1μl), Company D (1μl), CSB-MA000021M0m (1μl) + Flag Tag in 293F transfected whole cell lysate (500μg)
Lane 3: Flag Tag in 293F transfected whole cell lysate (20μg) -
Immunofluorescence staining of 293T cells transfected with Flag tag by CSB-MA000021M0m at 1:300, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-mouse IgG(H+L). The image on the right is the 293T cells transfected without Flag tag.
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其他：
產品詳情
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產品描述：CUSABIO貨號：CSB-MA000021M0m Flag標簽單克隆抗體是針對DYKDDDDK氨基酸序列開發的高特異性科研工具，該八肽標簽因其親水特性和可定向插入蛋白N端/C端的特性，已成為重組蛋白表達驗證的核心分子標記。本產品采用穩定雜交瘤技術制備，具備跨物種廣譜反應性，可兼容ELISA、Western Blotting、免疫熒光和免疫沉淀四大實驗平臺，特別適用于融合蛋白表達效率驗證、亞細胞定位追蹤及蛋白互作復合物捕獲等研究場景。作為FLAG標簽檢測的關鍵試劑，該抗體在HEK293細胞重組表達體系驗證中展現出優異的信噪比性能，配合抗體重鏈特異性設計有效避免輕鏈干擾，為基因編輯驗證、信號通路蛋白互作研究提供精準的分子檢測解決方案。
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別名：DDK tag, DYKDDDDK, Flag, Flag Tag
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宿主：Mouse
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反應種屬：All
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免疫原：DYKDDDDK(Flag) synthetic peptide conjugate to KLH
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標記方式：Non-conjugated
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克隆類型：Monoclonal
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抗體亞型：IgG2a
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純化方式：>95%, Protein G purified
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克隆號：4G5C8
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濃度：It differs from different batches. Please contact us to confirm it.
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保存緩沖液：Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 -
產品提供形式：Liquid
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應用范圍：ELISA, WB, IF, IP
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推薦稀釋比：
Application Recommended Dilution WB 1:1000-1:10000 IF 1:100-1:300 IP 0.5ul-2ul -
Protocols：
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儲存條件：Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
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貨期：Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
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用途：For Research Use Only. Not for use in diagnostic or therapeutic procedures.
引用文獻
- The Lyn/RUVBL1 Complex Promotes Colorectal Cancer Liver Metastasis by Regulating Arachidonic Acid Metabolism through Chromatin Remodeling Z Zhang, Y Gao, Y Qian, B Wei, K Jiang, Z Sun, F Zhang, M Yang, S Baldi, X Yu, Y Zuo,Advanced science,2025
- Angiopoietin-like protein 8 (betatrophin) inhibits hepatic gluconeogenesis through PI3K/Akt signaling pathway in diabetic mice Z Zhao,Metabolism: clinical and experimental,2023
- Stomatin modulates adipogenesis through the ERK pathway and regulates fatty acid uptake and lipid droplet growth SC Wu,Nature communications,2022
- Galectin‐1–RNA interaction map reveals potential regulatory roles in angiogenesis J Wei,FEBS letters,2021
- The role of the cytoplasmic tail of the influenza virus M2 protein in viral morphology and replication Badham M, et al,University of Kent,2018
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