HA-Tag Monoclonal Antibody
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中文名稱：HA-Tag鼠單克隆抗體
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貨號：CSB-MA000141M0m
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規格：￥400
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圖片：
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Western Blot
Positive WB detected in: 3 different overexpression lysates with HA tagged
All lanes: HA-Tag antibody at 1:1000
Secondary
Goat polyclonal to Mouse IgG at 1/10000 dilution
Predicted band size: 35, 35, 48 kDa
Observed band size: 35, 35, 48 kDa -
Western Blot
Positive WB detected in: HA-tagged fusion protein at 50ng, 25ng, 12.5ng, 6.25ng, 3.125ng, 1.5625ng
All lanes: HA-Tag antibody at 1:1000
Secondary
Goat polyclonal to Mouse IgG at 1/10000 dilution
Predicted band size: 35 kDa
Observed band size: 35 kDa -
Western Blot
Positive WB detected in: HA-tagged fusion protein
All lanes: HA-Tag antibody at 1:5000, 1:10000, 1:20000, 1:40000, 1:80000, 1:160000
Secondary
Goat polyclonal to Mouse IgG at 1/10000 dilution
Predicted band size: 35 kDa
Observed band size: 35 kDa -
Western Blot
Positive WB detected in: 6 different recombinant proteins with HA tagged, Hela whole cell lysate, 3T3 whole cell lysate
All lanes: HA-Tag antibody at 1:5000
Secondary
Goat polyclonal to Mouse IgG at 1/10000 dilution -
Immunofluorescence staining of 293F cells with CSB-MA000141M0m at 1:100, counter-stained with DAPI. The cells were blocked in 10% normal Goat Serum and then incubated with the primary antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Mouse IgG(H+L).
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Immunofluorescence staining of 293F transfected cells with CSB-MA000141M0m at 1:100, counter-stained with DAPI. The cells were blocked in 10% normal Goat Serum and then incubated with the primary antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Mouse IgG(H+L).
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Immunofluorescence staining of 293F cells and 293F transfected cells with Company A, Company B, Company C, Company D, Company E, CSB-MA000141M0m at 1:100, counter-stained with DAPI. The cells were blocked in 10% normal Goat Serum and then incubated with the primary antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Mouse IgG(H+L).
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Immunoprecipitating HA-Tag in 293F transfected whole cell lysate
Lane 1: Mouse control IgG (1µg) instead of CSB-MA000141M0m in 293F transfected whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000)
Lane 2: CSB-MA000141M0m (5µg) + 293F transfected whole cell lysate (500µg)
Lane 3: 293F transfected whole cell lysate (20µg) -
Immunoprecipitating HA-Tag in 293F transfected whole cell lysate
Lane 1: Mouse control IgG (1µg) instead of CSB-MA000141M0m in 293F transfected whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000)
Lane 2: Company A (5µg), Company B (5µg),Company C (5µg),Company D (5µg),Company E (5µg),CSB-MA009476A0m (5µg) + 293F transfected whole cell lysate (500µg)
Lane 3: 293F transfected whole cell lysate (20µg) -
Overlay histogram showing 293F transfected cells stained with CSB-MA000141M0m (red line) at 1:100. The cells were incubated in 1x PBS /10% normal goat serum to block non-specific protein-protein interactions followed by primary antibody for 1 h at 4°C. The secondary antibody used was FITC goat anti-mouse IgG(H+L) at 1/200 dilution for 1 h at 4°C. Isotype control antibody (green line) was used under the same conditions. Acquisition of >10,000 events was performed.
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其他：
產品詳情
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產品描述：CUSABIO貨號：CSB-MA000141M0m HA-Tag單克隆抗體是針對血凝素標簽（HA-Tag）研發的高特異性科研試劑，該表位標簽廣泛應用于重組蛋白標記領域，通過特異性結合實現目標蛋白的精準追蹤與功能解析。本產品采用雜交瘤技術制備，經嚴格驗證可識別天然及變性狀態下的HA表位，適用于ELISA、Western Blot、免疫熒光、流式細胞術和免疫共沉淀等多種實驗體系，且具備跨物種廣譜反應性，可兼容哺乳動物、昆蟲等多種表達系統構建的HA融合蛋白檢測需求。在蛋白質相互作用研究、基因表達調控分析、信號轉導通路驗證等前沿科研場景中表現優異，特別適用于融合蛋白純化效率驗證、亞細胞定位追蹤以及蛋白質復合物組分鑒定等關鍵實驗環節，為分子生物學、細胞生物學及蛋白質組學研究提供可靠工具。該抗體經多批次質控確保批間穩定性，實驗方案靈活適配不同檢測平臺需求。
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別名：HA-Tag
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宿主：Mouse
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反應種屬：All
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免疫原：YPYDVPDYA synthetic peptide conjugate to KLH
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標記方式：Non-conjugated
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克隆類型：Monoclonal
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抗體亞型：IgG2b
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純化方式：>95%, Protein A purified
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克隆號：18B11H6
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濃度：It differs from different batches. Please contact us to confirm it.
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保存緩沖液：Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4 -
產品提供形式：Liquid
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應用范圍：ELISA, WB, IF, IP, FC
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推薦稀釋比：
Application Recommended Dilution WB 1:5000-1:160000 IF 1:50-1:200 IP 1µg-5µg FC 1:50-1:200 -
Protocols：
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儲存條件：Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
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貨期：Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
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用途：For Research Use Only. Not for use in diagnostic or therapeutic procedures.
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