TUBA1A Monoclonal Antibody

中文名稱：

TUBA1A鼠單克隆抗體
貨號(hào)：

CSB-MA754656A0m
規(guī)格：

￥400
圖片：
Western Blot
Positive WB detected in: U87 whole cell lysate, PC-3 whole cell lysate, 293 whole cell lysate, U251 whole cell lysate, A549 whole cell lysate, A375 whole cell lysate, MG-63 whole cell lysate, SH-SY5Y whole cell lysate,
All lanes: TUBA1A antibody at 1:5000
Secondary
Goat polyclonal to Mouse IgG at 1/10000 dilution
Predicted band size: 52 kDa
Observed band size: 52 kDa

Western Blot
Positive WB detected in: Rabbit heatrt tissue, Rabbit liver tissue, Rabbit spleen tissue, Rabbit lung tissue, Rabbit kidney tissue, Rabbit small intestine tissue, Rabbit skeletal muscle tissue
All lanes: TUBA1A antibody at 1:5000
Secondary
Goat polyclonal to Mouse IgG at 1/10000 dilution
Predicted band size: 52 kDa
Observed band size: 52 kDa

Western Blot
Positive WB detected in: Rat brain tissue, Rat stomach tissue, Rat kidney tissue
All lanes: TUBA1A antibody at 1:5000
Secondary
Goat polyclonal to Mouse IgG at 1/10000 dilution
Predicted band size: 52 kDa
Observed band size: 52 kDa

Western Blot
Positive WB detected in: NIH/3T3 whole cell lysate, RAW264.7 whole cell lysate, Mouse brain tissue, Mouse kidney tissue
All lanes: TUBA1A antibody at 1:5000
Secondary
Goat polyclonal to Mouse IgG at 1/10000 dilution
Predicted band size: 52 kDa
Observed band size: 52 kDa

Western Blot
Positive WB detected in: Hela whole cell lysate at 20μg, 10μg, 5μg, 2.5μg, 1.25μg
All lanes: TUBA1A antibody at 1:5000
Secondary
Goat polyclonal to Mouse IgG at 1/10000 dilution
Predicted band size: 52 kDa
Observed band size: 52 kDa

Western Blot
Positive WB detected in: Hela whole cell lysate
All lanes: TUBA1A antibody at 1:2500, 1:5000, 1:10000, 1:20000, 1:40000, 1:80000, 1:160000
Secondary
Goat polyclonal to Mouse IgG at 1/10000 dilution
Predicted band size: 52 kDa
Observed band size: 52 kDa

Western Blot
Positive WB detected in: Hela whole cell lysate at 20μg, 10μg, 5μg, 2.5μg, 1.25μg, 0.625μg, 0.3125μg, 0.15625μg
All lanes: Company A, Company B, Company C, Company D, CSB-MA754656A0m antibody at 1:5000
Secondary
Goat polyclonal to Mouse IgG at 1/10000 dilution
Predicted band size: 52 kDa
Observed band size: 52 kDa

Western Blot
Positive WB detected in: Hela whole cell lysate
All lanes: Company A, Company B, Company C, Company D, CSB-MA754656A0m antibody at 1:2500, 1:5000, 1:10000, 1:20000, 1:40000, 1:80000, 1:160000, 320000
Secondary
Goat polyclonal to Mouse IgG at 1/10000 dilution
Predicted band size: 52 kDa
Observed band size: 52 kDa

IHC image of CSB-MA754656A0m diluted at 1:150 and staining in paraffin-embedded human tonsil tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

IHC image of CSB-MA754656A0m diluted at 1:150 and staining in paraffin-embedded human breast cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

Immunofluorescence staining of Hela cells with CSB-MA754656A0m at 1:75, counter-stained with DAPI. The cells were blocked in 10% normal Goat Serum and then incubated with the primary antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Mouse IgG(H+L).

Immunoprecipitating TUBA1A in Hela whole cell lysate
Lane 1: Mouse control IgG (1μg) instead of CSB-MA754656A0m in Hela whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000)
Lane 2: CSB-MA754656A0m (5μg) + Hela whole cell lysate (500μg)
Lane 3: Hela whole cell lysate (20μg)

Immunoprecipitating TUBA1A in Hela whole cell lysate
Lane 1: Mouse control IgG (1μg) instead of CSB-MA754656A0m in Hela whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000)
Lane 2: Company A (5μg), Company B (5μg), Company C (5μg), Company D (5μg), CSB-MA754656A0m (5μg) + Hela whole cell lysate (500μg)
Lane 3: Hela whole cell lysate (20μg)

Overlay histogram showing Hela cells stained with CSB-MA754656A0m (red line) at 1:150. The cells were incubated in 1x PBS /10% normal goat serum to block non-specific protein-protein interactions followed by primary antibody for 1 h at 4°C. The secondary antibody used was FITC goat anti-mouse IgG(H+L) at 1/200 dilution for 1 h at 4°C. Isotype control antibody (green line) was used under the same conditions. Acquisition of >10,000 events was performed.

Overlay histogram showing Hela cells stained with Company A (5μg), Company B (5μg), Company C (5μg), Company D (5μg), CSB-MA754656A0m (5μg) (red line) at 1:150. The cells were incubated in 1x PBS /10% normal goat serum to block non-specific protein-protein interactions followed by primary antibody for 1 h at 4°C. The secondary antibody used was FITC goat anti-mouse IgG(H+L) at 1/200 dilution for 1 h at 4°C. Isotype control antibody (green line) was used under the same conditions. Acquisition of >10,000 events was performed.
其他：

產(chǎn)品詳情
產(chǎn)品評(píng)價(jià)
引用文獻(xiàn)
相關(guān)產(chǎn)品
靶點(diǎn)詳情

產(chǎn)品詳情

產(chǎn)品描述：

TUBA1A單克隆抗體（CUSABIO貨號(hào)：CSB-MA754656A0m）是科研領(lǐng)域檢測(cè)微管蛋白α-1A鏈（TUBA1A）的高特異性工具，該靶標(biāo)作為細(xì)胞骨架核心成分參與微管動(dòng)態(tài)組裝、有絲分裂及神經(jīng)元遷移調(diào)控，其功能異常與大腦皮質(zhì)發(fā)育障礙及神經(jīng)系統(tǒng)疾病密切相關(guān)。本產(chǎn)品采用雜交瘤技術(shù)制備，經(jīng)嚴(yán)格驗(yàn)證可識(shí)別人類、兔、大鼠及小鼠樣本中的TUBA1A蛋白，適用于多種實(shí)驗(yàn)場(chǎng)景：通過Western Blot精準(zhǔn)檢測(cè)細(xì)胞或組織裂解液中的TUBA1A表達(dá)水平，利用免疫組化（IHC）定位腦組織切片中的蛋白分布特征，結(jié)合免疫熒光（IF）觀察活細(xì)胞內(nèi)微管網(wǎng)絡(luò)形態(tài)，或借助流式細(xì)胞術(shù)（FC）進(jìn)行表面標(biāo)記分析。在神經(jīng)發(fā)育研究、細(xì)胞周期調(diào)控實(shí)驗(yàn)及疾病模型構(gòu)建中，該抗體可配合ELISA、免疫共沉淀（IP）等多元技術(shù)方案，為探索微管相關(guān)分子機(jī)制提供可靠支持，尤其適用于大腦畸形病理分析、神經(jīng)元遷移障礙研究及神經(jīng)退行性疾病機(jī)制等前沿科研領(lǐng)域。
產(chǎn)品名稱：

Mouse anti-Homo sapiens (Human) TUBA1A Monoclonal Antibody antibody
Uniprot No.：

Q71U36
基因名：

TUBA1A
別名：

Alpha tubulin 3 antibody; Alpha-tubulin 3 antibody; B alpha 1 antibody; FLJ25113 antibody; LIS3 antibody; TBA1A_HUMAN antibody; TUBA1A antibody; TUBA3 antibody; Tubulin alpha 1a antibody; Tubulin alpha 1A chain antibody; Tubulin alpha 3 antibody; Tubulin alpha 3 chain antibody; Tubulin alpha brain specific antibody; Tubulin alpha-1A chain antibody; Tubulin alpha-3 chain antibody; Tubulin B alpha 1 antibody; Tubulin B-alpha-1 antibody
宿主：

Mouse
反應(yīng)種屬：

Human, Rabbit, Rat, Mouse
免疫原：

A synthesized peptide derived from human Tubulin alpha-1A chain (297-309aa)
免疫原種屬：

Homo sapiens (Human)
標(biāo)記方式：

Non-conjugated
克隆類型：

Monoclonal Antibody
抗體亞型：

IgG2a
純化方式：

>95%, Protein A purified
克隆號(hào)：

7E5C12
濃度：

It differs from different batches. Please contact us to confirm it.
保存緩沖液：

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
產(chǎn)品提供形式：

Liquid
應(yīng)用范圍：

ELISA, WB, IHC, IF, FC, IP

推薦稀釋比：

Application	Recommended Dilution
WB	1:20000-1:320000
IHC	1:100-1:300
IF	1:50-1:200
FC	1:100-1:300
IP	1µg-5µg

Protocols：

ELISA Protocol
Western Blotting (WB) Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol
Flow cytometry (FC) Protocol
Immunoprecipitation (IP) Protocol
儲(chǔ)存條件：

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
貨期：

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
用途：

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

引用文獻(xiàn)

An Image-Based High-Throughput and High-Content Drug Screening Method Based on Microarray and Expansion Microscopy J Xie,ACS nano,2023

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

功能：

Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain.
基因功能參考文獻(xiàn)：
1. A de novo heterozygous c.320A>G [p.(His 107 Arg)] mutation in TUBA1A was identified in a patient with microcephaly, epileptic seizures, and severe developmental delay. PMID: 29109381
2. Given that Spastin engages the MT in two places, we propose that severing occurs by forces exerted on the C-terminal tail of tubulin, which results in a conformational change in tubulin, which releases it from the polymer. PMID: 17389232
3. Molecular docking studies revealed that 6f interacted and bound ef fi ciently with the colchicine-binding site of tubulin. In addition, 6f treatment induced G2/M cell cycle arrest dose-dependently and subsequently induced cell apoptosis PMID: 28440465
4. induced pluripotent stem cells (iPSCs) from the umbilical cord and peripheral blood of two lissencephaly patients with different clinical severities carrying alpha tubulin (TUBA1A) missense mutations, were generated. PMID: 27431206
5. Long intergenic non-coding RNA APOC1P1-3 inhibits apoptosis by decreasing alpha-tubulin acetylation in breast cancer. PMID: 27228351
6. Results show that Tuba1a plays an essential, noncompensated role in neuronal saltatory migration in vivo and highlight the importance of microtubule flexibility in nucleus-centrosome coupling and neuronal-branching regulation during neuronal migration. PMID: 28687665
7. data suggest that the TUBA1A mutations disrupting lateral interactions have pronounced dominant-negative effects on microtubule dynamics that are associated with the severe end of the lissencephaly spectrum PMID: 26493046
8. Data show that tubulin phosphorylation and acetylation play important roles in the control of microtubule assembly and stability. PMID: 26165356
9. Data show that plasma membrane Ca(2+)-ATPase (PMCA) was associated with tubulin in normotensive and hypertensive erythrocytes. PMID: 26307527
10. Studies indicate that alpha-tubulin acetylation and microtubule level is mainly governed by opposing actions of alpha-tubulin acetyltransferase 1 (ATAT1) and histone deacetylase 6 (HDAC6). PMID: 26227334
11. Data from studies using peptide fragment of alpha-tubulin (residues 31-49) suggest that Ser38 is crucial for substrate recognition by alpha-tubulin acetylase 1 (ATAT1); Asp39, Ile42, the glycine stretch (residues 43-45), and Asp46 are also involved. PMID: 25602620
12. Lysine 40 acetylation of alpha-tubulin does not result in significant changes in kinesin-1's landing rate or motility parameters. PMID: 24940781
13. These results demonstrated that SelP interacts with tubulin, alpha 1a (TUBA1A). PMID: 24914767
14. This study show all foetuses with lissencephaly and cerebellar hypoplasia carried distinct TUBA1A mutations. PMID: 25059107
15. These findings call attention to PKC-mediated phosphorylation of alpha-tubulin as a novel mechanism for controlling the dynamics of microtubules that result in cell movement. PMID: 24574051
16. case provides new insight into the wide spectrum of disease phenotypes associated with TUBA1A mutation PMID: 23528852
17. The present study confirms that mutations in tubulin genes are responsible for complex brain malformation. PMID: 24392928
18. Studies suggest that tubulin-interactive agents have the potential to play a significant role in the fight against cancer. PMID: 23818224
19. Missense mutations in TUBA1A were found in 3 patients with polymicrogyria. PMID: 22948023
20. We described the clinical course and pathological findings in a child with TUBA1A mutation PMID: 22633752
21. TUBA1A and TUBB2B coding regions have been sequenced that are associated with cortical malformations. PMID: 23361065
22. Data show that Na(+),K(+)-ATPase activity was >50% lower and membrane-associated tubulin content was >200% higher in erythrocyte membranes from diabetic patients. PMID: 22565168
23. study describes a 14-month-old girl with TUBA1A mutation-associated lissencephaly, and summarize the clinical and neuroradiologic findings of 19 cases in the literature PMID: 22264709
24. Alpha2B-adrenergic receptor interaction with tubulin controls its transport from the endoplasmic reticulum to the cell surface PMID: 21357695
25. The expression of alpha-tubulin and MDR1 may play an important role in the development and progression of human non-small cell lung carcinoma. PMID: 20510079
26. We report a mutation in TUBA1A as a cause of polymicrogyria. So far, all mutations in TUBA1A have occurred de novo, resulting in isolated cases. This article describes familial recurrence of TUBA1A mutations due to somatic mosaicism in a parent. PMID: 21403111
27. Data show that IAV-infected cells contain elevated level of AcTub and alpha-tubulin. PMID: 21094644
28. Mutations in TUBA1A result in defects in the tubulin folding and heterodimer assembly. PMID: 20603323
29. LIS-associated mutations of TUBA1A operate via diverse mechanisms that include disruption of binding sites for microtubule-associated proteins. PMID: 20466733
30. the dipole moments of each tubulin isotype may influence their functional characteristics within the cell, resulting in differences for MT assembly kinetics and stability PMID: 16941085
31. Mutations in alpha-tubulin in mice and humans that affect neuronal migration result in abnormal lamination of brain structures with associated behavioral deficits. PMID: 17218254
32. Retrospective examination of MR images suggests that patients with TUBA1A mutations share not only cortical dysgenesis, but also cerebellar, hippocampal, corpus callosum, and brainstem abnormalities PMID: 17584854
33. Increased expression of tubulin alpha is associated with pulmonary sclerosing hemangioma PMID: 17914564
34. The diminished production of TUBA1A tubulin in R264C individuals is consistent with haploinsufficiency as a cause of the disease phenotype. PMID: 18199681
35. the TUBA1A phenotype is distinct from LIS1, DCX, RELN and ARX lissencephalies. Compared with the phenotypes of children mutated for TUBA1A, these prenatally diagnosed fetal cases occur at the severe end of the TUBA1A lissencephaly spectrum. PMID: 18669490
36. Missense mutations within the TUBA1A gene are associated with specific abnormalities in lissencephaly. PMID: 18728072
37. mutation analysis in the TUBA1A gene in 46 patients with classical lissencephaly. PMID: 18954413
38. This protein has been found differentially expressed in the Wernicke's Area from patients with schizophrenia. PMID: 19405953
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相關(guān)疾病：

Lissencephaly 3 (LIS3)
亞細(xì)胞定位：

Cytoplasm, cytoskeleton.
蛋白家族：

Tubulin family
組織特異性：

Expressed at a high level in fetal brain.
數(shù)據(jù)庫(kù)鏈接：

HGNC: 20766

OMIM: 602529

KEGG: hsa:7846

STRING: 9606.ENSP00000301071

UniGene: Hs.654422

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