Recombinant Mouse Tumor necrosis factor receptor superfamily member 5 (Cd40), partial (Active)

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品名簡稱：

Recombinant Mouse Cd40 protein, partial (Active)
貨號：

CSB-MP004936MO1
說明書：

MSDS Datasheet
規格：

￥828
圖片：
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.

Activity
Measured by its binding ability in a functional ELISA.Immobilized Mouse Cd40 at 2 μg/ml can bind Mouse Cd40lg(CSB-MP004937MO1).The EC₅₀ is 5.121-6.085 ng/mL. Biological Activity Assay
其他：

產品詳情
產品評價
相關產品
靶點詳情

產品詳情

純度：

Greater than 95% as determined by SDS-PAGE.
內毒素：

Less than 1.0 EU/ug as determined by LAL method.
生物活性：

Measured by its binding ability in a functional ELISA.Immobilized Mouse Cd40 at 2 μg/mL can bind Mouse Cd40lg(CSB-MP004937MO1).The EC50 is 5.121-6.085 ng/mL.
基因名：

Cd40
Uniprot No.：

P27512
別名：

Tumor necrosis factor receptor superfamily member 5; B-cell surface antigen CD40; Bp50; CD40L receptor; CD40; Cd40; Tnfrsf5
種屬：

Mus musculus (Mouse)
蛋白長度：

Partial
來源：

Mammalian cell
分子量：

46.8 kDa
表達區域：

20-193aa
氨基酸序列

LGQCVTCSDKQYLHDGQCCDLCQPGSRLTSHCTALEKTQCHPCDSGEFSAQWNREIRCHQHRHCEPNQGLRVKKEGTAESDTVCTCKEGQHCTSKDCEACAQHTPCIPGFGVMEMATETTDTVCHPCPVGFFSNQSSLFEKCYPWTSCEDKNLEVLQKGTSQTNVICGLKSRMR
蛋白標簽：

C-terminal hFc-tagged
產品提供形式：

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
緩沖液：

Lyophilized from a 0.2 μm sterile filtered PBS, 6% Trehalose, pH 7.4
復溶：

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
儲存條件：

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
保質期：

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
貨期：

3-7 business days
注意事項：

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA：

Please contact us to get it.

產品評價

靶點詳情

功能：

Receptor for TNFSF5/CD40LG. Transduces TRAF6- and MAP3K8-mediated signals that activate ERK in macrophages and B cells, leading to induction of immunoglobulin secretion.
基因功能參考文獻：
1. these studies identify a PPARgamma-dependent miR-424/503-CD40 signaling axis that is critical for regulation of inflammation mediated angiogenesis PMID: 28566713
2. Data indicate a CD40-dependent mechanism capable of abrogating receptor-related orphan gammat(+) (RORgammat(+)) Helios(-)-induced Treg (iTreg) cells induction by CD103(+) dendritic cells (DCs). PMID: 28276457
3. Although either BCR or CD40 ligation induced c-Myc in naive B cells, both signals were required to highly induce c-Myc, a critical mediator of GC B cell survival and cell cycle reentry. PMID: 29396161
4. These results suggest that CD40-activated CD40L reverse signalling has striking and opposite effects on the growth and elaboration of dendrites among major classes of brain neurons by PKC-dependent mechanisms. PMID: 29111976
5. Results suggest that platelet CD40 plays a pivotal role in neointima formation after arterial injury PMID: 29037856
6. CD73 expression identifies a subset of IgM(+) antigen-experienced cells with memory attributes that is T cell and CD40 signalling dependent PMID: 28746783
7. CD40 silencing with a specific siRNA ameliorates progression of experimental atherosclerosis in ApoE(-/-) mice via NF-kappaB/miR-125b signaling axis. PMID: 27835742
8. CD40 functions as a non-redundant mechanism to convert the tumor microenvironment immunologically. PMID: 27292635
9. CD40 plays a critical role in the pathogenesis of type 1 diabetes, influencing lymphocyte trafficking, T-cell receptor expression, and T-cell pathogenesis. PMID: 28542921
10. role with b7 in primary germinal center generation of distinct antigen-presenting cells PMID: 28768709
11. CD40 signaling in adipose tissue macrophages regulates major histocompatibility complex class II and CD86 expression to control the expansion of CD4(+) T cells. PMID: 26658005
12. Adoptively transferred Th40 cells (CD4+CD40+ Tcells) are present in lesions in the CNS and are associated with wide spread demyelination. PMID: 28192476
13. this study reports that anti-CD40 mAb administration <3 d in advance of chemotherapy is lethal in more than half of treated C57BL/6 mice in pancreatic cancer model PMID: 27217585
14. The data show that CD40-mediated inhibition of PC generation is via engagement of multiple pathway and constitutive CD40 signaling in vivo involving bystander T-B interactions can calibrate B cell differentiation outcomes PMID: 27342845
15. BAFF upregulates CD28/B7 and CD40/CD154 expression, and promotes the interactions between T and B cells in a BAFF-R-dependent manner PMID: 27180986
16. CD40 in Muller cells is sufficient to upregulate retinal inflammatory markers and appears to promote experimental diabetic retinopathy and that Muller cells orchestrate inflammatory responses in myeloid cells through a CD40-ATP-P2X7 pathway. PMID: 27474370
17. Taken together, our data demonstrate the importance of CD40 signaling in the conversion of CTL exhaustion and its ability to enhance PD-1 antagonist action in rescuing exhausted CTLs in chronic infection. PMID: 28153727
18. Upon miR-155 overexpression, Cd40 expression was significantly upregulated with a negative correlation to the miR-155 primary target Ship1 expression in mice. PMID: 27509492
19. These results suggest that Il23a expression in DCs is synergistically triggered by the PG E2-EP4-cAMP-PKA pathway and canonical/non-canonical NF-kappaB pathways and CREB activated by CD40 stimulation. PMID: 26189370
20. Results suggest that altered CD40 signaling and granulocyte recruitment in response to infection are responsible for the Ity5-associated Salmonella susceptibility of A/J mice. PMID: 26562079
21. our results highlight new roles for CD40 and cysteine-238-mediated CD40 homodimers in cell biology and identify a potential new target for therapeutic strategies against CD40-associated chronic inflammatory diseases. PMID: 25977307
22. Latent virus infection upregulates CD40 expression facilitating enhanced autoimmunity in a model of multiple sclerosis PMID: 26356194
23. Platelet CD40 plays a crucial role in inflammation by stimulating leukocyte activation and recruitment and activation of endothelial cells, thereby promoting atherosclerosis. PMID: 26821950
24. there is a major involvement of the CD40-CD40L signaling pathway in long-term brain dysfunction in an animal model of sepsis. PMID: 25822797
25. NIK, and thus probably the noncanonical NF-kappaB pathway, is critical to allow DCs to acquire the capacity to cross-present antigen and prime CD8 T cells after exposure to licensing stimuli. PMID: 26561586
26. C57-CD40L(-/-) mouse is a useful surrogate model of X-HIGM syndrome for studying immune responses elicited against pathogens PMID: 26064940
27. although treated CD40 knockout mice remained healed, developed long-term immunity, and were resistant to secondary Leishmania major challenge, treated CD40L knockout reactivated their lesion after cessation of rIL-12 treatment. PMID: 26304989
28. Anti-OX40L mAb could prolong secondary heart allograft survival based on CD40/CD40L and LFA-1/ICAM-1 blockade. PMID: 25613092
29. CD40 on lymph node fibroblasts facilitated bidirectional communication with CD4(+) T cells via CD40-CD40L, thereby altering fibroblast gene expression of immune regulatory molecules. PMID: 25856408
30. CD40L mediates neointima formation via its classic receptor CD40 rather than via its recently described novel interaction with Mac-1. PMID: 24652469
31. p62 regulates CD40-mediated NF-kappaB activation in macrophages through interaction with TRAF6. PMID: 26133577
32. Although LIGHT is critical for maintenance of primary Th1 response, it is dispensable during secondary anti-Leishmania immunity in the presence of functional CD40 signaling. PMID: 26026056
33. Velcro" engineering of high affinity CD47 ectodomain as signal regulatory protein alpha (SIRPalpha) antagonists that enhance antibody-dependent cellular phagocytosis PMID: 25837251
34. findings indicate that in a setting of acute hepatitis, tumor-induced hepatic myeloid-derived suppressor cel act as proinflammatory immune effector cells capable of killing hepatocytes in a CD40-dependent manner. PMID: 25616156
35. These data reveal the L. major-enhanced CD40-induced N-Ras activation as a novel immune evasion strategy and the potential for Ras isoform-targeted antileishmanial immunotherapy and immunoprophylaxis. PMID: 25786685
36. Data indicate a role of scaffold protein JLP in the bridging of CD40 antigen internalization and CD40-dependent signaling in B lymphocytes. PMID: 25586186
37. CD40 is required for protective immunity against liver stage Plasmodium infection. PMID: 25646303
38. demonstrates that the simultaneous silencing of CD40 and CD80 genes has synergistic effects in preventing allograft rejection, and may therefore have therapeutic potential in clinical transplantation PMID: 24886282
39. an SHP-1-centered feedback system wherein SHP-1 modulates CD40-induced p38MAPK activation threshold and reciprocal ERK-1/2 activation, establishing itself as a critical regulator of CD40 signaling reciprocity PMID: 25187664
40. results imply that any setting of T cell lymphopenia or reduced CD40 function, including B cell recovery following transplantation, will impact the naive B cell repertoire PMID: 25172502
41. These results indicate that CD40 plays distinct roles in different tissues and, unexpectedly, plays an important role in maintaining immune homeostasis in epididymal adipose tissue. PMID: 24647739
42. CD40 signaling activates naive B cells to a phenotype that is intermediate between the naive and plasma cell stages of the B cell differentiation. PMID: 24482285
43. gammadelta T cells might activate dendritic cells via CD40 signalling during infection. Dendritic cell activation via gammadelta T cells expressing CD40 ligand is critical during the early phase of infection with Plasmodium berghei XAT. PMID: 24815981
44. We present the surprising finding that CD40 deficiency on T cells aggravates whereas activation of CD40 signaling improves adipose tissue inflammation and its metabolic complications. PMID: 24664276
45. Fas signals act as a silencing mechanism for CD40-induced RAGs and prevent CD40 translocation to the nucleus. PMID: 24037181
46. Data indicate that CD40-CD154 interactions drive the functional activation of steady-state dendritic cells. PMID: 24420080
47. Data indicate that CD40 stimulation enhanced only TLR-9 expression. PMID: 24387292
48. Data suggest that simultaneous knock-down of CD40 and IL-23p19 production by bone marrow-derived DCs (BMDCs) may represent a therapeutic tool for the treatment of IL-17-dependent autoimmune diseases, including multiple sclerosis. PMID: 24387596
49. CD40 plays a crucial role as an important costimulatory molecule in experimental autoimmune neuritis. PMID: 24709684
50. The CD40/CD40L-assisted crosstalk between mesenchymal stromal cells and mast cells in the splenic marginal zone lymphoma (SMZL) microenvironment finds correlation in p53(-/-) mice developing SMZL. PMID: 24452203
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亞細胞定位：

[Isoform I]: Cell membrane; Single-pass type I membrane protein.; [Isoform III]: Cell membrane; Single-pass type I membrane protein.; [Isoform IV]: Cell membrane; Single-pass type I membrane protein.; [Isoform V]: Cell membrane; Single-pass type I membrane protein.; [Isoform II]: Secreted.
數據庫鏈接：

KEGG: mmu:21939

STRING: 10090.ENSMUSP00000017799

UniGene: Mm.271833