CD40
CD40，即腫瘤壞死因子受體超家族成員5（TNFRSF5），別名Bp50、CD40L等。CD40由人類CD40基因編碼，廣泛表達于免疫細胞，特別是B細胞、樹突狀細胞（DC）和單核細胞。CD40是一種單鏈型跨膜蛋白，包括一個胞外區(qū)域、一個跨膜區(qū)域和一個胞漿區(qū)域，其胞外區(qū)域可以與其配體CD40L（CD40配體）結(jié)合，觸發(fā)信號傳導。CD40在連接先天免疫和適應性免疫方面發(fā)揮著重要作用，其功能擴展到B細胞激活、抗原呈遞和炎癥調(diào)節(jié)等多個層面。CD40對于體液免疫應答、免疫記憶的形成以及免疫調(diào)節(jié)都具有重要意義。CD40信號傳導也在免疫治療研究中被廣泛研究，以開發(fā)新的治療方法來增強或抑制免疫系統(tǒng)的特定響應。
熱銷產(chǎn)品
驗證數(shù)據(jù)
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
Activity
Measured by its binding ability in a functional ELISA. Immobilized CD40 at 2 μg/ml can bind CD40L (CSB-MP004937HU3), the EC50 is 3.112-3.858 ng/ml.
Activity
Human CD40 protein hFc tag (CSB-MP004936HU1) captured on COOH chip can bind Human CD40L protein hFc and Flag tag (CSB-MP004937HU3) with an affinity constant of 2.06 nM as detected by LSPR Assay.
The purity of CD40 was greater than 95% as determined by SEC-HPLC
CD40 Recombinant Monoclonal Antibody (CSB-RA004936MA1HU)
驗證數(shù)據(jù)
Immunofluorescence staining of A549 cell with CSB-RA004936MA1HU at 1:30, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-mouse IgG(H+L).
Immunofluorescence staining of PC-3 cell with CSB-RA004936MA1HU at 1:30, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-mouse IgG(H+L).
Overlay Peak curve showing THP-1 cells surface stained with CSB-RA004936MA1HU (red line) at 1:100. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*106cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated Goat Anti-Mouse IgG(H+L) at 1/200 dilution for 35 min at 4°C. Isotype control antibody (green line) was mouse IgG1 (1μg/1*106cells) used under the same conditions. Acquisition of >10,012 events was performed.
CD40 Antibodies
CD40 for Homo sapiens (Human)
| 產(chǎn)品貨號 | 產(chǎn)品名稱 | 種屬反應性 | 應用類型 |
|---|---|---|---|
| CSB-PA004936GA01HU | CD40 Antibody | Human | ELISA,WB |
| CSB-PA176134 | Phospho-CD40 (Thr254) Antibody | Human,Mouse | ELISA,WB |
| CSB-PA001455 | CD40 Antibody | Human,Mouse,Monkey | WB, IHC, ELISA |
| CSB-PA080031 | Phospho-CD40 (T254) Antibody | Human,Mouse | WB, ELISA |
| CSB-PA215588 | CD40 Antibody | Human,Mouse | ELISA,IHC |
| CSB-PA004936LA01HU | CD40 Antibody | Human, Mouse | ELISA, WB, IF |
| CSB-PA004936LB01HU | CD40 Antibody, HRP conjugated | Human | ELISA |
| CSB-PA004936LC01HU | CD40 Antibody, FITC conjugated | Human | |
| CSB-PA004936LD01HU | CD40 Antibody, Biotin conjugated | Human | ELISA |
| CSB-RA004936MA1HU | CD40 Recombinant Monoclonal Antibody | Human | ELISA, IF, FC |
CD40 Proteins
CD40 Proteins for Mus musculus (Mouse)
| 產(chǎn)品貨號 | 產(chǎn)品名稱 | 來源 |
|---|---|---|
| CSB-YP004936MO1 CSB-EP004936MO1 CSB-BP004936MO1 CSB-EP004936MO1-B |
Recombinant Mouse Tumor necrosis factor receptor superfamily member 5 (Cd40), partial | Yeast E.coli Baculovirus In Vivo Biotinylation in E.coli |
| CSB-CF004936MO | Recombinant Mouse Tumor necrosis factor receptor superfamily member 5 (Cd40) | in vitro E.coli expression system |
CD40 Proteins for Homo sapiens (Human)
| 產(chǎn)品貨號 | 產(chǎn)品名稱 | 來源 |
|---|---|---|
| CSB-YP004936HU1 CSB-EP004936HU1 CSB-EP004936HU1-B |
Recombinant Human Tumor necrosis factor receptor superfamily member 5 (CD40), partial | Yeast E.coli In Vivo Biotinylation in E.coli |
| CSB-CF004936HU | Recombinant Human Tumor necrosis factor receptor superfamily member 5 (CD40) | in vitro E.coli expression system |
| CSB-AP005171HU | Recombinant Human Tumor necrosis factor receptor superfamily member 5 (CD40), partial (Active) | Mammalian cell |
| CSB-AP005181HU | Recombinant Human Tumor necrosis factor receptor superfamily member 5 (CD40), partial (Active) | Mammalian cell |
| CSB-MP004936HU1 | Recombinant Human Tumor necrosis factor receptor superfamily member 5 (CD40), partial (Active) | Mammalian cell |
| CSB-BP004936HU1 | Recombinant Human Tumor necrosis factor receptor superfamily member 5 (CD40), partial | Baculovirus |
CD40 Proteins for Bos taurus (Bovine)
| 產(chǎn)品貨號 | 產(chǎn)品名稱 | 來源 |
|---|---|---|
| CSB-YP636713BO CSB-EP636713BO CSB-BP636713BO CSB-MP636713BO CSB-EP636713BO-B |
Recombinant Bovine Tumor necrosis factor receptor superfamily member 5 (CD40), partial | Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli |
CD40 Proteins for Callithrix jacchus (White-tufted-ear marmoset)
| 產(chǎn)品貨號 | 產(chǎn)品名稱 | 來源 |
|---|---|---|
| CSB-YP659466CYL CSB-EP659466CYL CSB-BP659466CYL CSB-MP659466CYL CSB-EP659466CYL-B |
Recombinant Callithrix jacchus Tumor necrosis factor receptor superfamily member 5 (CD40), partial | Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli |
CD40 Proteins for Equus caballus (Horse)
| 產(chǎn)品貨號 | 產(chǎn)品名稱 | 來源 |
|---|---|---|
| CSB-YP675871HO CSB-EP675871HO CSB-BP675871HO CSB-MP675871HO CSB-EP675871HO-B |
Recombinant Horse Tumor necrosis factor receptor superfamily member 5 (CD40), partial | Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli |
CD40 Proteins for Canis familiaris (Dog) (Canis lupus familiaris)
| 產(chǎn)品貨號 | 產(chǎn)品名稱 | 來源 |
|---|---|---|
| CSB-YP767175DO CSB-EP767175DO CSB-BP767175DO CSB-MP767175DO CSB-EP767175DO-B |
Recombinant Dog Tumor necrosis factor receptor superfamily member 5 (CD40), partial | Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli |
CD40 Proteins for Sus scrofa (Pig)
| 產(chǎn)品貨號 | 產(chǎn)品名稱 | 來源 |
|---|---|---|
| CSB-BP851448PI CSB-MP851448PI CSB-EP851448PI-B |
Recombinant Pig Tumor necrosis factor receptor superfamily member 5 (CD40), partial | Baculovirus Mammalian cell In Vivo Biotinylation in E.coli |
CD40 ELISA Kit
CD40 ELISA Kit for Mus musculus (Mouse)
| 產(chǎn)品貨號 | 產(chǎn)品名稱 | 樣本類型 | 靈敏度 |
|---|---|---|---|
| CSB-EL004936MO | Mouse Tumor necrosis factor receptor superfamily member 5(CD40) ELISA kit | serum, plasma, tissue homogenates | 7.8 pg/ml |
