Rat Renin ELISA Kit

Rat Renin ELISA Kit

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中文名稱：

大鼠腎素(Renin)酶聯(lián)免疫試劑盒
貨號(hào)：

CSB-E08702r
規(guī)格：

96T/48T
價(jià)格：

￥3900/￥2500
其他：

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產(chǎn)品描述：

大鼠腎素(Renin)酶聯(lián)免疫試劑盒（CSB-E08702r）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的REN含量。REN作為一種新興的腫瘤治療靶點(diǎn)，其研究背景主要圍繞腫瘤的發(fā)生發(fā)展機(jī)制。研究機(jī)制上，REN通過調(diào)控細(xì)胞增殖、免疫逃逸、血管生成等關(guān)鍵生物學(xué)過程，為腫瘤治療提供了新的思路。試劑盒檢測范圍為15.6 mU/mL-1000 mU/mL，適用于心血管疾病動(dòng)物模型研究、腎功能評估、藥物干預(yù)效果驗(yàn)證等科研場景本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
別名：

Ren1 ELISA Kit; Ren ELISA Kit; Renin ELISA Kit; EC 3.4.23.15 ELISA Kit; Angiotensinogenase ELISA Kit
縮寫：

Renin
Uniprot No.：

P08424
種屬：

Rattus norvegicus (Rat)
樣本類型：

serum, plasma, tissue homogenates
檢測范圍：

15.6 mU/mL-1000 mU/mL
靈敏度：

3.9 mU/mL
反應(yīng)時(shí)間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領(lǐng)域：

Cardiovascular
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rat renin in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	94
	Range %	90-99
1:2	Average %	90
	Range %	85-94
1:4	Average %	99
	Range %	92-104
1:8	Average %	88
	Range %	84-92

回收率：

The recovery of rat renin spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	90-100
EDTA plasma (n=4)	97	92-102

標(biāo)準(zhǔn)曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

mU/ml	OD1	OD2	Average	Corrected
1000	2.497	2.382	2.440	2.254
500	1.773	1.641	1.707	1.521
250	1.152	1.199	1.176	0.990
125	0.693	0.738	0.716	0.530
62.5	0.479	0.515	0.497	0.311
31.2	0.318	0.309	0.314	0.128
15.6	0.234	0.217	0.226	0.040
0	0.191	0.180	0.186

數(shù)據(jù)處理：

ELISA操作視頻及標(biāo)曲繪制軟件下載
貨期：

3-5 working days

Erythropoietin alleviates post-resuscitation myocardial dysfunction in rats potentially through increasing the expression of angiotensin II receptor type 2 in myocardial tissues Zhou H,Molecular medicine reports,2023
Effects of Long-Term Intervention with Losartan, Aspirin and Atorvastatin on Vascular Remodeling in Juvenile Spontaneously Hypertensive Rats Q Liu,Molecules,2023
Peperomia pellucida (L.) Kunth as an Angiotensin-converting Enzyme Inhibitor in Two-kidney, One-clip Goldblatt Hypertensive Rats FC Saputri,Saudi Journal of Biological Sciences,2021
Paroxetine Attenuates Cardiac Hypertrophy Via Blocking GRK2 and ADRB1 Interaction in Hypertension X Sun,Journal of the American Heart Associatio,2021
Irradiation of carotid baroreceptor with low‐intensity pulsed ultrasound exerts different metabolic protection in perirenal, epididymal white adipose tissue and interscapular brown adipose tissue of obese rats Q Cao,FASEB J,2020
Effects of Hibiscus Sabdariffa Calyces Aqueous Extract on the Antihypertensive Potency of Captopril in the Two-Kidney-One-Clip Rat Hypertension Model Shinta Ayu Nurfaradilla, et al,Evidence-Based Complementary and Alternative Medicine,2019
Effects of Sleeve Gastrectomy on Blood Pressure and the Renal Renin-Angiotensin System in Rats with Diet-Induced Obesity Yoshida Y, et al,Obesity,2019
Chios mastic gum decreases renin levels and ameliorates vascular remodeling in renovascular hypertensive rats Aspasia I.Tzani.et al,Biomedicine & Pharmacotherapy,2018
The effect of ozone on blood pressure in DOCA-salt-induced hypertensive rats Akc謀lar R. et al,Int J Clin Exp Med.,2015
The role of KCa3. 1 channels in cardiac fibrosis induced by pressure overload in rats Zhao LM et al,Pflugers Arch,2015
Dietary sodium intake modulates renal excretory responses to intrarenal angiotensin (1-7) administration in anesthetized rats O'Neill J et al,Am J Physiol Regul Integr Comp Physiol,2013

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功能：

Renin is a highly specific endopeptidase, whose only known function is to generate angiotensin I from angiotensinogen in the plasma, initiating a cascade of reactions that produce an elevation of blood pressure and increased sodium retention by the kidney.
基因功能參考文獻(xiàn)：
1. The influence of intracellular renin on the inward calcium current in isolated smooth muscle cells from SHR mesenteric arteries was investigated... intracellular renin in vascular resistance vessels from SHR due to internalization or expression, contributes to the regulation of vascular tone and control of peripheral resistance-an effect independently of Ang II. PMID: 27545826
2. An alternative promoter in intron1 of the renin gene is regulated by glucose starvation via SRF. PMID: 28715805
3. renin deficiency leads to significant dysregulation of ion transporters. PMID: 27443990
4. Renal ischemia/reperfusion injury induces renin response in the kidney. Renin production is more explicit in males than in females which, via increased angiotensin II production, might explain differences in renal vessel regulation between the two genders. PMID: 27090360
5. Isolated perfused hearts obtained from transgenic rats overexpressing cytosolic renin exhibited a 50 % reduction of infarct size after ischemia-reperfusion injury. PMID: 26256830
6. plasma level elevated in experimental colitis PMID: 26660905
7. elevated levels of circulating (pro)renin in model of pre-eclampsia PMID: 25767135
8. prorenin has distinct pro-angiogenic and pro-inflammatory effects on retinal cells PMID: 25707593
9. Report alterations in adrenal gland aldosterone/corticosterone biosynthesis in the renin knockout rat. PMID: 25394830
10. at least 4 BP loci reside within the 30-cM chromosome 13 BP quantitative trait locus that includes Renin PMID: 23460292
11. Up-regulation of renin might contribute to abnormal programming of renal growth in rats exposed to postnatal early overnutrition. PMID: 21752621
12. An association of post-natal overfeeding and high-fat diet increases plasma renin activity and adipose (pro)renin receptor expression. PMID: 21483231
13. This study examined angiogenic function and genomic structure of regions surrounding the renin gene. PMID: 21521778
14. Although plasma renin activity was suppressed in the angiotensinogen II-infused rats, renin content in the renal medulla was increased. Renin levels in the urine increased. PMID: 21865264
15. Renin released from mast cells activated by circulating MCP-1 initiates the microvascular phase of the systemic inflammation of alveolar hypoxia. PMID: 21963836
16. The transfer of chromosome 13 from BN to SS rats (to produce the SS.13BN strain) has cardiovascular effects. PMID: 22020141
17. Imidazolone deposition and renin production in peritubular capillary endothelial cells may play a role in the development of tubulointerstitial fibrosis in insulin-resistant hypertensive rats. PMID: 21484732
18. Cu/Zn SOD expression was significantly higher in cerebral arteries from Ren1-Brown Norway congenic rats vs. those from Dahl salt-sensitive rats PMID: 21331057
19. The development of the first rat knockout in the renin-angiotensin system emphasizes the role of renin in BP regulation and kidney function even in the low-renin SS rat. PMID: 21242461
20. Dahl SS renin allele plays a crucial role in endothelial dysfunction present in the cerebral circulation of the Dahl SS rat, even in the absence of elevated dietary salt intake. PMID: 20852041
21. The data demonstrate that a cytosolic renin exists in cardiomyocytes, which in contradiction to secretory renin protects from necrosis but increases apoptosis. PMID: 18671756
22. Nitric oxide synthase mRNA levels do not correlate with gene expression of renin in selected brain areas PMID: 11702851
23. Coronary dilatation reserve in experimental hypertension and chronic heart failure: effects of blockade of the renin-angiotensin system PMID: 11903302
24. cellular location of thymic renin is mainly in macrophages and epithelial cells and is greater in hypertensive rats PMID: 12242043
25. mRNA expression is related to renal dysfunction in tacrolimus-induced acute nephrotoxicity. PMID: 12469222
26. Intracranial renin alters gustatory neural responses in the nucleus of the solitary tract of rats. PMID: 12511427
27. cyclooxygenase 2 activity controls the renin-angiotensin system and contributes to elevated renin in the postnatal period PMID: 12560203
28. renin-specific antibodies demonstrate that cardiac mast cells contain renin PMID: 15342908
29. Results suggest that the nonproteolytic activation of prorenin may be a significant mechanism of diabetic nephropathy. PMID: 15489960
30. show that the intracellular domain of Notch1, transcription factor Ets-1, and homeo box D10 (HOXD10).pre B-cell leukemia transcription factor 1(PBX1b).PREP1 activate the rat renin promoter cooperatively PMID: 15792957
31. the augmentation of distal nephron renin in ANG II-infused hypertensive rats is AT1 receptor mediated PMID: 15870381
32. Angiotensin II-mediated oxidative stress promotes myocardial tissue remodeling in the transgenic (mRen2) 27 Ren2 rat. PMID: 17440033
33. Effect of the renin gene on skeletal muscle angiogenesis induced by electrical stimulation. PMID: 18198281
34. Release of mast cell renin triggers locally produced angiotensin II-induced contraction of rat bronchial rings in vitro. PMID: 18202178
35. in a rat model of diabetes, prorenin content and release from the collecting duct may be more important than the juxtaglomerular apparatus in contrast to the existing paradigm PMID: 18413493
36. Upregulation of distal tubular renin helps to explain how sustained intrarenal angiotensin II formation occurs even during juxtaglomerular renin suppression PMID: 18426992
37. augmented activation of the renin-angiotensin system may be related to enhanced sympathetic outflow observed in sedentary animals and may contribute to increased risk of cardiovascular disease in the sedentary population PMID: 18509102
38. Renin inhibition attenuates insulin resistance, oxidative stress, and pancreatic remodeling in the transgenic Ren2 rat. PMID: 18653711
39. Exon(1A-9) renin transcript (1) is translated into a functionally active intracellular protein; (2) is targeted to the cytoplasm rather than being sorted to the secretory pathways and (3) is functionally active, regulating aldosterone production. PMID: 18782187
40. Adipose tissue may control its own local renin concentration independently of plasma renin as a potential mechanism for maintaining a functional local adipose renin-angiotensin system. PMID: 19050177
41. mineralocorticoid receptor antagonism attenuates glomerular/podocyte remodeling and albuminuria, in part through reductions in redox-mediated impairment of insulin metabolic signaling in Ren2 rats PMID: 19261739
42. Ang peptide formation consequent to the increased prorenin levels prevented reduction of Ang II levels after binephrectomy and was likely to have contributed to hypertension, cardiac hypertrophy, and suppression of kidney renin levels in transgenic rats PMID: 19841286
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亞細(xì)胞定位：

Secreted. Membrane.
蛋白家族：

Peptidase A1 family
數(shù)據(jù)庫鏈接：

KEGG: rno:24715

STRING: 10116.ENSRNOP00000003951

UniGene: Rn.9831

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.