Mouse Interleukin 2,IL-2 ELISA kit
產(chǎn)品詳情
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產(chǎn)品描述：
小鼠白介素2(IL-2)酶聯(lián)免疫試劑盒（CSB-E04627m-IS）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿、組織培養(yǎng)上清液樣本中的IL2含量。IL-2（白細胞介素-2）是一種重要的免疫調節(jié)因子，最初被命名為T細胞生長因子（TCGF），屬于Ⅰ型細胞因子家族。它主要由CD4+ T細胞產(chǎn)生，通過JAK-STAT、ERK和PI3K信號通路激活免疫細胞。IL-2受體由α、β、γ三條鏈組成，其中β/γ鏈是下游信號通路激活所必需的。IL-2在免疫系統(tǒng)中具有多種功能，包括增強細胞溶解活性、驅動Treg細胞的發(fā)育、抑制免疫細胞的應答等。試劑盒檢測范圍為3.9 pg/ml-250 pg/ml，靈敏度為0.975 pg/ml。科研人員可利用探究感染性疾病模型、自身免疫疾病機制、腫瘤免疫微環(huán)境調控等領域的IL - 2分泌規(guī)律，特別適合評估藥物干預、基因編輯或免疫刺激后IL - 2的濃度變化本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
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別名：
Il2 ELISA kit; Il-2Interleukin-2 ELISA kit; IL-2 ELISA kit; T-cell growth factor ELISA kit; TCGF ELISA kit
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縮寫：
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Uniprot No.：
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種屬：
Mus musculus (Mouse)
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樣本類型：
serum, plasma, tissue homogenates, cell culture supernates
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檢測范圍：
3.9 pg/ml-250 pg/ml
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靈敏度：
0.975 pg/ml
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反應時間：
1-5h
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樣本體積：
50-100ul
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檢測波長：
450 nm
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研究領域：
Immunology
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測定原理：
quantitative
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測定方法：
Sandwich
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精密度：
Intra-assay Precision (Precision within an assay): CV%<8% | | | |
Three samples of known concentration were tested twenty times on one plate to assess. | |
Inter-assay Precision (Precision between assays): CV%<10% | | | |
Three samples of known concentration were tested in twenty assays to assess. | | |
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線性度：
To assess the linearity of the assay, samples were spiked with high concentrations of mouse IL-2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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| Sample | Serum(n=4) | |
1:1 | Average % | 89 | |
Range % | 84-95 | |
1:2 | Average % | 92 | |
Range % | 85-99 | |
1:4 | Average % | 98 | |
Range % | 91-105 | |
1:8 | Average % | 93 | |
Range % | 86-98 | |
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回收率：
The recovery of mouse IL-2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
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Sample Type | Average % Recovery | Range | |
Serum (n=5) | 94 | 87-101 | |
EDTA plasma (n=4) | 88 | 81-95 | |
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標準曲線：
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
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pg/ml | OD1 | OD2 | Average | Corrected | |
250 | 2.278 | 2.234 | 2.256 | 2.104 | |
125 | 1.443 | 1.467 | 1.455 | 1.303 | |
62.5 | 0.904 | 0.888 | 0.896 | 0.744 | |
31.25 | 0.473 | 0.499 | 0.486 | 0.334 | |
15.6 | 0.341 | 0.321 | 0.331 | 0.179 | |
7.8 | 0.245 | 0.253 | 0.249 | 0.097 | |
3.9 | 0.208 | 0.198 | 0.203 | 0.051 | |
0 | 0.149 | 0.155 | 0.152 | | |
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本試劑盒所含材料：
- A micro ELISA plate ---The 96-well plate has been pre-coated with an anti-mouse IL-2 antibody. This dismountable microplate can be divided into 12 x 8 strip plates.
- Two vials lyophilized standard ---Dilute a bottle of the standard at dilution series, read the OD values, and then draw a standard curve.
- One vial Biotin-labeled IL-2 antibody (100 x concentrate) (120 μl/bottle) ---Act as the detection antibody.
- One vial HRP-avidin (100 x concentrate) (120 μl/bottle) ---Bind to the detection antibody and react with the TMB substrate to make the solution chromogenic.
- One vial Biotin-antibody Diluent (15 ml/bottle) ---Dilute the Biotin-antibody.
- One vial HRP-avidin Diluent (15 ml/bottle) ---Dilute the HRP-avidin solution.
- One vial Sample Diluent (50 ml/bottle)---Dilute the sample to an appropriate concentration.
- One vial Wash Buffer (25 x concentrate) (20 ml/bottle) ---Wash away unbound or free substances.
- One vial TMB Substrate (10 ml/bottle) ---Act as the chromogenic agent. TMB interacts with HRP, eliciting the solution turns blue.
- One vial Stop Solution (10 ml/bottle) ---Stop the color reaction. The solution color immediately turns from blue to yellow.
- Four Adhesive Strips (For 96 wells) --- Cover the microplate when incubation.
- An instruction manual
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本試劑盒不含材料：
- A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm or 570 nm.
- An incubator can provide stable incubation conditions up to 37°C±5°C.
- Centrifuge
- Vortex
- Squirt bottle, manifold dispenser, or automated microplate washer
- Absorbent paper for blotting the microtiter plate
- 50-300ul multi-channel micropipette
- Pipette tips
- Single-channel micropipette with different ranges
- 100ml and 500ml graduated cylinders
- Deionized or distilled water
- Timer
- Test tubes for dilution
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數(shù)據(jù)處理：
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貨期：
3-5 working days
靶點詳情
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功能：
Produced by T-cells in response to antigenic or mitogenic stimulation, this protein is required for T-cell proliferation and other activities crucial to regulation of the immune response. Can stimulate B-cells, monocytes, lymphokine-activated killer cells, natural killer cells, and glioma cells.
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基因功能參考文獻：
- These studies demonstrate that Th1 CD4(+) T cells require IL-2 for lung T resident memory cell development. PMID: 28948612
- Because IL-2 production was limited to cells receiving the strongest T cell receptor (TCR) signals, a direct link between TCR-signal strength, IL-2 production, and T cell fate determination has been established. PMID: 30213884
- These data highlights the existence of IL-2 trans-presentation between NK cells in the local microenvironment where the availability of IL-2 is limited. PMID: 28074895
- interleukin-2 (IL-2) is a non-pancreatic autoimmune target in type 1 diabetes PMID: 27708334
- Each mutation decreased STAT5 binding and altered IL-2-induced Il2ra gene expression, revealing that individual elements within the superenhancer were not functionally redundant and that all were required for normal gene expression. PMID: 29078395
- Deleting IL-2 in CD11c(high)MHCII(+) cells induces spontaneous colitis resembling human inflammatory bowel disease. PMID: 29549257
- a significant increase in plasma levels of IL-2, IFN-g and TNF-g was revealed as assessed by ELISA. In conclusion, the results of the present study indicate that MENK has a cytotoxic effect on B16 melanoma cells in vitro and in vivo, and suggest a potential mechanism for these bioactivities. PMID: 28849104
- WASp knockout mice controlled growth of A20 lymphoma cells that naturally produced IL-2. PMID: 27477778
- Tumor growth delays observed by tumor irradiation combined with MVA-MUC1-IL-2 vaccine were significantly more prolonged than those observed by vaccine, radiation, or radiation with MVA empty vector. PMID: 28116088
- IL-2 signalling is essential to prevent deletion of CD4SP CCR7+ Helios+ thymocytes at a later developmental stage than Card11 is required to prevent deletion. The deletion prevented by IL-2 signalling occurs in a Foxp3-independent manner. PMID: 28362433
- in vivo targeting of the TNF superfamily receptor TNFRSF25 using the TL1A-Ig fusion protein, along with IL-2, resulted in transient but massive Treg expansion in donor mice; transplantation of Treg-expanded donor cells facilitated transplant tolerance without GVHD, with complete sparing of graft-versus-malignancy. PMID: 28219835
- IL-2 and IL-6 work together to enhance influenza-specific CD8 T cell generation responding to live influenza virus in aged mice and humans PMID: 27322555
- this report, we elucidated the unsolved mechanism of the anti-cancer effect of curcumin by identifying IL-2 as a direct molecular target. Curcumin, as a small molecule IL-2 modulator, has the potential to be used to treat IL-2 related pathologic conditions. PMID: 29127008
- Data show that PTEN plays a key role in Th17 cell differentiation by blocking IL-2 expression. PMID: 29018045
- AnxA6 regulates IL-2 homeostasis and sensitivity in T cells by sustaining a lipid raft-like membrane environment. PMID: 26853809
- NOD Treg cells have an impaired responsiveness to IL-2 that reduces their ability to compete for a limited supply of IL-2. PMID: 26763864
- Suppression of IL-12p70 formation by IL-2 or following macrophage depletion causes T-cell autoreactivity leading to CNS demyelination in HSV-1-infected mice. PMID: 28542613
- At single cell level, IL-2 is binary (digital) and CD25 is graded expressed, whereas at population level both parameters show graded expression correlating with the antigen amount. PMID: 28035902
- IL-2/anti-IL-2 complexes protected lupus-prone mice against lupus nephritis by expanding Tregs. PMID: 27914701
- results show that IL-23 accounts for the main aspects of human and murine lupus including the expansion of double negative T cells, decreased IL-2, and increased IL-17 production PMID: 28646040
- studies revealed a new epigenetic pathway in the control of IL-2 production in systemic lupus erythematosus whereby low levels of miR-200a-3p accumulate the binding of the ZEB1-CtBP2 complex to the IL-2 promoter and suppress IL-2 production PMID: 28438897
- data suggest that the use of Golgi transport inhibitors results in an underestimation of the presence of type 2 cytokine-secreting cells and highlight IL-2 as a critical component in optimal cytokine production by Th2 and Th9 cells in vitro and in vivo PMID: 28468971
- These results suggested that TSC-22 could counteract the protective effect of GILZ on IL-2-deprivation-induced apoptosis. PMID: 26752201
- this study shows that IL-2 modulates the TCR signaling threshold for CD8 but not CD4 T cell Proliferation on a single-cell level PMID: 28159902
- These data demonstrate an important synergetic role of TGF-beta and IL-2 in the generation, activation and stability of Treg cells, as well as their subsequent development into follicular regulatory T cells. PMID: 27787514
- Mecamylamine, a nAChR inhibitor, suppressed not only these [Ca(2+)]i transients, but also IL-2 release and T cell proliferation PMID: 28025040
- Flavonoid glycosides of Alchornea floribunda did not result in detectable Il2 secretion by treated splenic T-lymphocytes. PMID: 26974045
- We also discuss the role of interleukin 2 (IL-2), which is decisive for the function of Treg and has been used therapeutically in preliminary trials in human SLE. The identification of novel Treg markers and the development of novel therapeutic approaches, which restore the balance between Treg and autoreactive Tcells are future goals for research in SLE. PMID: 26975190
- Data show that after tumor necrosis factor alpha-induced protein 8 like-2 (TIPE2) gene was down-regulated, the expression of the CD69 antigen was increased, and the proliferation of T lymphocytes and the secretion of cytokines IL-2 and IFN-gamma were enhanced. PMID: 27363266
- This study evaluated a chemical genetic toolkit that evaluated a biphasic requirement for JAK3 kinase activity in IL-2-driven T cell proliferation. PMID: 27018889
- Dominance of regulatory T cells in carcinogen-induced fibrosarcomas is not T-bet or Il-2 dependent. PMID: 26433463
- TCF1 is required for the T follicular helper cell response to viral infection functioning through negative feedback loops with IL-2 and Blimp1. PMID: 26365183
- These results may provide an additional understanding of the characteristics of the various fractions of isolated Tregs based on phenotype and function and the role of varying levels of exogenous IL-2 on the suppressive activity of these cells. PMID: 26529512
- findings demonstrate that distinct niches within the lymphoid organ T zone support distinct cell fate decisions, and they establish a function for dendritic-cell-derived CD25 in controlling IL-2 availability and T-cell differentiation PMID: 27147029
- IL-2 signaling modulates TH1 cell, follicular helper T cell and central memory T cell gene expression. PMID: 26743592
- Ndrg1 is a T-cell clonal anergy factor negatively regulated by IL-2. PMID: 26507712
- Innate cell-derived IL-2 is a critical cofactor in regulating innate lymphoid cell function in pulmonary type 2 pathology PMID: 26025126
- glucosamine interferes with N-glycosylation of CD25, and thereby attenuates IL-2 downstream signaling PMID: 26468284
- Interleukin-2 critically regulates bone marrow erythropoiesis and prevents anemia development. PMID: 26404745
- findings identified that autocrine IL-2 production operates in a dose-dependent fashion to facilitate the expansion potential of Ag-specific CD8(+) T cell populations, which may instigate ways to augment therapies depending on fit CD8(+) T cells. PMID: 26453748
- IL-2-mediated activation of the Akt kinase and mTORc1 signaling was both necessary and sufficient to shift differentiation away from Tfh cells, instead promoting that of Th1 cells. PMID: 26410627
- This study identified a novel long-range enhancer of the Il2 gene located 83 kb upstream of the transcription start site. PMID: 26351138
- The amelioration by Gl-PS against the suppression of the production of IL-2, IFN-gamma and TNF-alpha in mononuclear lymphocytes by B16F10 cell culture supernatant might contribute to cancer control. PMID: 25585987
- Late IL-2 promotes survival through acute downregulation of apoptotic pathways in effector T cells and by permanently upregulating their IL-7 receptor expression, enabling IL-7 to sustain them as memory T cells. PMID: 25369785
- enhances anti-CD45RBmAb-induced immune tolerance to skin allograft via up-regulated T regulatory cells PMID: 25550088
- Chronodependent effect of interleukin-2 on mouse spleen cells in the model of cyclophosphamide immunosuppression. PMID: 25708328
- Peripherally Induced Tolerance Depends on Peripheral Regulatory T Cells That Require Hopx To Inhibit Intrinsic IL-2 Expression. PMID: 26170384
- The morphologic changes and rapid cell death induced by dimeric IL-2 imply that cell death is mediated by disruption of membrane permeability and subsequent necrosis. PMID: 25019288
- autocrine IL-2 signaling is functional in GM-CSF myeloid dendritic cells in an early time window after PAMPs stimulation PMID: 25652593
- IL-2 produced by antigen-bearing dendritic cells (DCs) had a key role in Treg cell development and that existing Treg cells limited new development of Treg cells by competing for IL-2. PMID: 25939026
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亞細胞定位：
Secreted.
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蛋白家族：
IL-2 family
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數(shù)據(jù)庫鏈接：
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