Rat P-Selectin ELISA kit
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中文名稱：大鼠P選擇素(P-Selectin/CD62P)酶聯(lián)免疫試劑盒
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貨號：CSB-E07399r
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規(guī)格：96T/48T
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價(jià)格：￥3900/￥2500
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其他：
產(chǎn)品詳情
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產(chǎn)品描述：大鼠P選擇素(P-Selectin/CD62P)酶聯(lián)免疫試劑盒（CSB-E07399r）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的SELP含量。SELP即選擇素P，主要表達(dá)于活化的血小板和內(nèi)皮細(xì)胞。它參與炎癥反應(yīng)、血栓形成等病理生理過程。研究機(jī)制方面，SELP通過與配體結(jié)合，介導(dǎo)白細(xì)胞、血小板與內(nèi)皮細(xì)胞的黏附，在腫瘤轉(zhuǎn)移、動(dòng)脈粥樣硬化等疾病機(jī)制中扮演重要角色。試劑盒檢測范圍為9.38 ng/mL-600 ng/mL，靈敏度為2.34 ng/mL。在科研領(lǐng)域，廣泛用于心血管疾病模型、炎癥反應(yīng)機(jī)制、血栓性疾病研究及藥物干預(yù)效果評估等方向，例如通過檢測動(dòng)物模型血液或組織中的P選擇素水平，揭示其在血管內(nèi)皮損傷、血小板活化等生理病理過程中的動(dòng)態(tài)變化，為相關(guān)基礎(chǔ)研究提供可靠數(shù)據(jù)支持。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
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別名：Selp; P-selectin; CD62 antigen-like family member P; Granule membrane protein 140; GMP-140; Leukocyte-endothelial cell adhesion molecule 3; LECAM3; Platelet activation dependent granule-external membrane protein; PADGEM; CD antigen CD62P
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縮寫：
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Uniprot No.：
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種屬：Rattus norvegicus (Rat)
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樣本類型：serum, plasma, tissue homogenates
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檢測范圍：9.38 ng/mL-600 ng/mL
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靈敏度：2.34 ng/mL
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反應(yīng)時(shí)間：1-5h
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樣本體積：50-100ul
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檢測波長：450 nm
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研究領(lǐng)域：Cardiovascular
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測定原理：quantitative
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測定方法：Sandwich
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精密度：
Intra-assay Precision (Precision within an assay): CV%<8% Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV%<10% Three samples of known concentration were tested in twenty assays to assess. -
線性度：
To assess the linearity of the assay, samples were spiked with high concentrations of rat P-selectin in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. Sample Serum(n=4) 1:1 Average % 93 Range % 85-99 1:2 Average % 92 Range % 84-105 1:4 Average % 97 Range % 92-103 1:8 Average % 94 Range % 82-98 -
回收率：
The recovery of rat P-selectin spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. Sample Type Average % Recovery Range Serum (n=5) 97 95-100 EDTA plasma (n=4) 95 92-100 -
標(biāo)準(zhǔn)曲線：
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. 
ng/ml OD1 OD2 Average Corrected 600 2.755 2.812 2.784 2.616 300 2.217 2.236 2.227 2.059 150 1.545 1.524 1.535 1.367 75 1.051 0.979 1.015 0.847 37.5 0.587 0.622 0.605 0.437 18.75 0.456 0.438 0.447 0.279 9.38 0.288 0.279 0.284 0.116 0 0.173 0.162 0.168 -
數(shù)據(jù)處理：
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貨期：3-5 working days
引用文獻(xiàn)
- Metformin regulates the effects of IR and IGF-1R methylation on mast cell activation and airway reactivity in diabetic rats with asthma through miR-152-3p/DNMT1 axis D Fu,Cell Biology and Toxicology,2023
- Docking of THPDTPI: to explore P-selectin as a common target of anti-tumor, anti-thrombotic and anti-inflammatory agent Zhu H,Oncotarget,2023
- Shape memory and hemostatic silk-laponite scaffold for alveolar bone regeneration after tooth extraction trauma H Zhou,International journal of biological macromolecules,2024
- DM-induced Hypermethylation of IR and IGF1R attenuates mast cell activation and airway responsiveness in rats D Fu,Journal of Cellular and Molecular Medicine,2020
- Heptapeptide-based modification leading to enhancing the action of MTCA on activated platelets, P-selectin, GPIIb/IIIa Ze Li.et al,FUTURE MED CHEM,2018
- Assessment of Thrombotic Risk in Atrial Fibrillation with Ultrasound Molecular Imaging of P-Selectin Jing Y.et al,Thromb Haemost,2018
- Protective role of 尾-patchoulene from Pogostemon cablin against indomethacin-induced gastric ulcer in rats: Involvement of anti-inflammation Jia-Zhen Wu.et al,Phytomedicine,2017
- IQCA-TAVV: To explore the effect of P-selectin, GPIIb/IIIa, IL-2, IL-6 and IL-8 on deep venous thrombosis Wu J.et al,Oncotarget,2017
- ATIQCTPC: a nanomedicine capable of targeting tumor and blocking thrombosis in vivo Xu X.et al,Int J Nanomedicine.,2017
- IQCA-TASS: a nano-scaled P-selectin inhibitor capable of targeting thrombus and releasing IQCA/TARGD (S) S in vivo Jianhui Wu.et al,Journal of Materials Chemistry B,2016
- Impaired NO-mediated vasodilatation in rat coronary arteries after asphyxial cardiac arrest. Troelsen TT.et al,Acta Anaesthesiol Scand.,2015
- Aqueous extract of Rabdosia rubescens leaves: forming nanoparticles, targeting P-selectin, and inhibiting thrombosis Wang Y. et al,Int J Nanomedicine,2015
- The application of tetrahydroisoquinoline-3-carbonyl-TARGD (F) F as an anti-thrombotic agent having dual mechanisms of action Yang G et al,Mol Biosyst,2012
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靶點(diǎn)詳情
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功能：Ca(2+)-dependent receptor for myeloid cells that binds to carbohydrates on neutrophils and monocytes. Mediates the interaction of activated endothelial cells or platelets with leukocytes. The ligand recognized is sialyl-Lewis X. Mediates rapid rolling of leukocyte rolling over vascular surfaces during the initial steps in inflammation through interaction with SELPLG.
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基因功能參考文獻(xiàn)：
- Aortic cyclooxygenase-2 and P-selectin were significantly down-regulated in plant sterols and plant sterols + tripeptides groups. PMID: 22575043
- Fibrinogen can interact with P-selectin in order to contribute to the development of atherosclerosis, high levels of fibrinogen and P-selectin can be regarded as risk factors for markers of atherosclerosis. PMID: 22340239
- Renal injury after ischemia/reperfusion may relate to the strong expression of P-selectin. NO may have a protective affect by decreasing the expression of P-selectin and alleviating the adhesion, aggregation and infiltration of neutrophils. PMID: 21180132
- Both baicalin and octreotide can exert some protective effects on multiple organs of rats with severe acute pancreatitis via decreased P-selectin expression. PMID: 19780886
- Volatile oil of Magnolia biondii can protect the kidney in rats with diabetic nephropathy by inhibiting the expressions of P-selectin protein in serum and in renal tissue. PMID: 18471420
- P-selectin, contribute to massive infiltration of Mo into the interstitium by functioning as specialized post-capillary venules. PMID: 12081568
- P-selectin was highly expressed on glomerular endothelial cells after injection of nephrotoxic serum.P-selectin partially mediates glomerular infiltration of macrophage in experimental crescentic glomerulonephritis. PMID: 12100021
- Blockade of this molecule protects rat steatotic liver grafts from ischemia/reperfusion injury PMID: 12201360
- P-selectin increases CD45-positive leukocyte activation, inflammation and neointimal formation in a Zucker diabetic rat carotid artery balloon injury model. PMID: 12377736
- Neointimal rat SMCs supported a 2.5-fold higher arrest of monocytes and "memory" T lymphocytes than medial SMCs, which was dependent on both P-selectin and VLA-4. PMID: 14963004
- P-selectin increased significantly in hepatic sinusoidal endothelial cells and renal tubular epithelial cells 1 h after ischemia-reperfusion PMID: 15742404
- expression of the P-selectin gene is induced through the activation of NF-kappaB and that P-selectin may be involved in the pathogenesis of diabetic nephropathy PMID: 15888973
- P-selectin plays a role in ischemia-reperfusion injury in experimental myocardial infarct. PMID: 17488661
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亞細(xì)胞定位：Cell membrane; Single-pass type I membrane protein.
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蛋白家族：Selectin/LECAM family
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組織特異性：Not detected in the absence of exposure to lipopolysaccharide (LPS). Detected only after exposure to lipopolysaccharide (LPS) in the tissues examined: spleen, lung, brain, liver, heart, kidney, thymus and small intestine.
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