Rabbit von Willebrand Factor,VWF ELISA Kit

產品詳情

說明書下載

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

產品描述：

兔血管性血友病因子/瑞斯托霉素輔因子(VWF)酶聯免疫試劑盒（CSB-E08525Rb）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿樣本中的VWF含量。VWF（血管性血友病因子）在止血和血栓形成中起關鍵作用。其背景上，VWF缺陷或異常會導致血管性血友病。研究機制方面，它能與血小板和內皮下膠原結合，介導血小板黏附、聚集，還參與凝血因子Ⅷ的運輸和保護，維持其活性。試劑盒檢測范圍為0.312 ng/mL-20 ng/mL，該產品廣泛應用于心血管疾病動物模型中的凝血機制研究、抗血栓藥物體外作用評估以及血小板功能相關的基礎科研實驗，可為血管內皮損傷、血栓形成等病理過程的分子機制探索提供數據支持，滿足實驗室對VWF蛋白定量分析的需求。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
縮寫：

VWF
種屬：

Oryctolagus cuniculus (Rabbit)
樣本類型：

serum, plasma
檢測范圍：

0.312 ng/mL-20 ng/mL
靈敏度：

0.078 ng/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Cardiovascular
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rabbit VWF in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	85
	Range %	80-92
1:2	Average %	95
	Range %	85-100
1:4	Average %	95
	Range %	87-106
1:8	Average %	93
	Range %	86-98

回收率：

The recovery of rabbit VWF spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	91-103
EDTA plasma (n=4)	94	86-100

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.