Rat von Willebrand Factor,vWF ELISA Kit

Rat von Willebrand Factor,vWF ELISA Kit

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中文名稱：

大鼠血管性血友病因子/瑞斯托霉素輔因子(VWF)酶聯(lián)免疫試劑盒
貨號：

CSB-E08438r
規(guī)格：

96T/48T
價格：

￥3600/￥2500
其他：

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產(chǎn)品描述：

大鼠血管性血友病因子/瑞斯托霉素輔因子(VWF)酶聯(lián)免疫試劑盒（CSB-E08438r）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的VWF含量。VWF即血管性血友病因子，在止血過程中發(fā)揮重要作用。其背景上與血管性血友病等疾病密切相關(guān)。研究機(jī)制方面，它能介導(dǎo)血小板黏附于受損血管壁，還可與凝血因子Ⅷ結(jié)合，保護(hù)其不被快速降解，目前圍繞它的研究有助于疾病診斷與治療。試劑盒檢測范圍為0.312 ng/mL-20 ng/mL，適用于心血管疾病模型構(gòu)建、血栓機(jī)制研究、藥物療效評估等科研場景。為探索VWF在生理病理過程中的作用提供高效工具。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
別名：

Vwf ELISA Kit; von Willebrand factor ELISA Kit; vWF ELISA Kit; Fragment ELISA Kit
縮寫：

VWF
Uniprot No.：

Q62935
種屬：

Rattus norvegicus (Rat)
樣本類型：

serum, plasma, tissue homogenates
檢測范圍：

0.312 ng/mL-20 ng/mL
靈敏度：

0.078 ng/mL
反應(yīng)時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領(lǐng)域：

Cardiovascular
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rat vWF in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:100	Average %	91
	Range %	86-99
1:200	Average %	97
	Range %	92-105
1:400	Average %	85
	Range %	81-87
1:800	Average %	88
	Range %	83-95

回收率：

The recovery of rat vWF spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	90	87-93
EDTA plasma (n=4)	95	90-100

標(biāo)準(zhǔn)曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
20	2.300	2.488	2.394	2.257
10	2.011	2.102	2.057	1.920
5	1.446	1.560	1.503	1.366
2.5	0.903	0.900	0.902	0.765
1.25	0.628	0.586	0.607	0.470
0.625	0.350	0.364	0.357	0.220
0.312	0.242	0.258	0.250	0.113
0	0.134	0.140	0.137

數(shù)據(jù)處理：

ELISA操作視頻及標(biāo)曲繪制軟件下載
貨期：

3-5 working days

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功能：

Important in the maintenance of hemostasis, it promotes adhesion of platelets to the sites of vascular injury by forming a molecular bridge between sub-endothelial collagen matrix and platelet-surface receptor complex GPIb-IX-V. Also acts as a chaperone for coagulation factor VIII, delivering it to the site of injury, stabilizing its heterodimeric structure and protecting it from premature clearance from plasma.
亞細(xì)胞定位：

Secreted. Secreted, extracellular space, extracellular matrix.
組織特異性：

Plasma.
數(shù)據(jù)庫鏈接：

STRING: 10116.ENSRNOP00000026643

UniGene: Rn.35561

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.