Rabbit cross linked C-telopeptide of type Ⅱ collagen (CTX-Ⅱ) ELISA Kit

Rabbit cross linked C-telopeptide of type Ⅱ collagen (CTX-Ⅱ) ELISA Kit

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中文名稱：

兔子Ⅱ型膠原C端肽(CTX-Ⅱ)酶聯(lián)免疫試劑盒
貨號：

CSB-E16213Rb
規(guī)格：

96T/48T
價格：

￥3800/￥2500
其他：

CUSABIO Wins 2024 Bioz Rapid Star? Award in the ELISA category

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產(chǎn)品描述：

兔子Ⅱ型膠原C端肽(CTX-Ⅱ)酶聯(lián)免疫試劑盒（CSB-E16213Rb）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的CTX-Ⅱ含量。CTX-Ⅱ是一種由Ⅱ型膠原降解產(chǎn)生的肽段，是骨關節(jié)炎（OA）的潛在生物標志物。其水平與關節(jié)損傷密切相關，可用于OA的早期診斷和病情評估。研究顯示，CTX-Ⅱ在OA患者關節(jié)液中水平升高，可作為疾病進展及預后的指標。試劑盒檢測范圍為0.625 ng/mL-40 ng/mL，常用于骨關節(jié)炎、類風濕性關節(jié)炎等疾病模型中軟骨退行性病變的機制研究。適用于科研場景中評估實驗動物軟骨代謝狀態(tài)、篩選保護軟骨組織的候選化合物，或探究病理條件下膠原降解的動態(tài)變化，可為體外實驗提供穩(wěn)定的數(shù)據(jù)支持，助力關節(jié)疾病相關的基礎研究與藥物開發(fā)。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
別名：

cross linked C-telopeptide of type 2 collagen (CTX-2),_x000D_ cross linked C-telopeptide of type II collagen (CTX-II)
縮寫：

CTX-Ⅱ
種屬：

Oryctolagus cuniculus (Rabbit)
樣本類型：

serum, plasma, tissue homogenates
檢測范圍：

0.625 ng/mL-40 ng/mL
靈敏度：

0.156 ng/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Others
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rabbit CTX-Ⅱ in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	95
	Range %	89-103
1:2	Average %	90
	Range %	86-99
1:4	Average %	95
	Range %	90-102
1:8	Average %	92
	Range %	83-99

回收率：

The recovery of rabbit CTX-Ⅱ spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	97	92-103
EDTA plasma (n=4)	95	89-106

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
40	2.547	2.652	2.600	2.462
20	2.037	1.962	2.000	1.862
10	1.449	1.376	1.413	1.275
5	0.902	0.866	0.884	0.746
2.5	0.514	0.526	0.520	0.382
1.25	0.332	0.347	0.340	0.202
0.625	0.246	0.253	0.250	0.112
0	0.139	0.136	0.138

數(shù)據(jù)處理：

ELISA操作視頻及標曲繪制軟件下載
貨期：

3-5 working days

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.