Mouse C-telopeptide of type Ⅱ collagen(CTX-Ⅱ) ELISA Kit

產(chǎn)品詳情

文獻(xiàn)引用 (4) 說(shuō)明書下載

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

產(chǎn)品描述：

小鼠Ⅱ型膠原C端肽(CTX-Ⅱ)酶聯(lián)免疫試劑盒（CSB-E16211m）為雙抗夾心法ELISA試劑盒，定量檢測(cè)血清、血漿、組織勻漿樣本中的CTX-Ⅱ含量。試劑盒檢測(cè)范圍為3.12 pg/mL-200 pg/mL，在骨關(guān)節(jié)炎模型研究、軟骨退行性病變機(jī)制探索及藥物干預(yù)效果評(píng)估中具有重要應(yīng)用價(jià)值；支持科研人員高效完成軟骨代謝相關(guān)疾病的動(dòng)物模型構(gòu)建、病理機(jī)制分析以及潛在治療靶點(diǎn)的驗(yàn)證工作；為關(guān)節(jié)炎、骨質(zhì)疏松等骨骼系統(tǒng)疾病的分子機(jī)制研究提供可靠工具。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說(shuō)明書。
別名：

C-telopeptide of type 2 collagen(CTX-2),_x000D_ C-telopeptide of type II collagen(CTX-II)
縮寫：

CTX-Ⅱ
種屬：

Mus musculus (Mouse)
樣本類型：

serum, plasma, tissue homogenates
檢測(cè)范圍：

3.12 pg/mL-200 pg/mL
靈敏度：

0.78 pg/mL
反應(yīng)時(shí)間：

1-5h
樣本體積：

50-100ul
檢測(cè)波長(zhǎng)：

450 nm
研究領(lǐng)域：

Others
測(cè)定原理：

quantitative
測(cè)定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of mouse CTX-Ⅱ in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:20	Average %	93
	Range %	86-95
1:40	Average %	99
	Range %	96-102
1:80	Average %	92
	Range %	87-95
1:160	Average %	96
	Range %	93-99

回收率：

The recovery of mouse CTX-Ⅱ spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	93	89-96
EDTA plasma (n=4)	94	91-96

標(biāo)準(zhǔn)曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

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