Pig secretory immunoglobulin A,SIgA ELISA Kit
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中文名稱：豬分泌型免疫球蛋白A(sIgA)酶聯(lián)免疫試劑盒
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貨號：CSB-E12063p
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規(guī)格：96T/48T
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價(jià)格：￥4200/￥3000
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其他：
產(chǎn)品詳情
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產(chǎn)品描述：豬分泌型免疫球蛋白A(sIgA)酶聯(lián)免疫試劑盒（CSB-E12063p）為競爭法ELISA試劑盒，定量檢測血清、血漿、組織培養(yǎng)上清液、組織勻漿樣本中的sIgA含量。sIgA即分泌型免疫球蛋白A，是黏膜免疫的主要效應(yīng)分子。其能在黏膜表面阻止病原體黏附與入侵，中和毒素等。研究中聚焦于其產(chǎn)生機(jī)制、影響因素等，以探索增強(qiáng)sIgA水平或功能的方法，用于防治黏膜感染等疾病。試劑盒檢測范圍為0.15 μg/mL-37.5 μg/mL，適用于動物免疫機(jī)制研究，如評估腸道感染模型中的黏膜免疫應(yīng)答、疫苗效價(jià)驗(yàn)證或飼料添加劑對免疫調(diào)節(jié)的影響等領(lǐng)域。支持科研人員在體外實(shí)驗(yàn)中系統(tǒng)性分析sIgA的動態(tài)變化，為豬類免疫學(xué)研究提供可靠工具。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
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縮寫：sIgA
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種屬：Sus scrofa (Pig)
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樣本類型：serum, plasma, cell culture supernates, tissue homogenates
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檢測范圍：0.15 μg/mL-37.5 μg/mL
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靈敏度：0.15 μg/mL
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反應(yīng)時(shí)間：1-5h
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樣本體積：50-100ul
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檢測波長：450 nm
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研究領(lǐng)域：Others
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測定原理：quantitative
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測定方法：Competitive
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精密度：
Intra-assay Precision (Precision within an assay): CV%<8% Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV%<10% Three samples of known concentration were tested in twenty assays to assess. -
線性度：
To assess the linearity of the assay, samples were spiked with high concentrations of pig sIgA in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. Sample Serum(n=4) 1:200 Average % 92 Range % 88-97 1:400 Average % 94 Range % 90-98 1:800 Average % 87 Range % 85-94 1:1600 Average % 96 Range % 91-103 -
回收率：
The recovery of pig sIgA spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. Sample Type Average % Recovery Range Serum (n=5) 95 89-99 EDTA plasma (n=4) 103 98-108 -
標(biāo)準(zhǔn)曲線：
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. 
μg/ml OD1 OD2 Average 37.5 0.120 0.115 0.118 9.38 0.456 0.463 0.460 2.34 0.901 0.877 0.889 0.58 1.432 1.527 1.480 0.15 2.103 2.189 2.146 0 2.544 2.753 2.649 -
數(shù)據(jù)處理：
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貨期：3-5 working days
引用文獻(xiàn)
- Supplementation with Kluyveromyces marxianus probiotic yeasts in sows and their piglets in early post-weaning: effects in biological and productive parameters MD Pendón, MF Compagnucci, E Valette, JI Girardi,research square,2025
- Effects of Magnolia officinalis extract on the growth performance and immune function of weaned piglets C Zhang, B Liu, Z Cui, K Wu, H Huang, Y Wang,Porcine Health Management,2025
- Beneficial Role of Heat‐Treated Lactobacillus sakei HS‐1 on Growth Performance, Nutritional Status and Gut Microbiota in Weaned Piglets K Matsubara, J Li, Y Enomoto,Journal of Animal Physiology and Animal Nutrition,2024
- Maternal resveratrol improves the intestinal health and weight gain of suckling piglets during high summer temperatures: The involvement of exosome-derived microRNAs and immunoglobin in colostrum C Hong,Animal nutrition,2024
- Intrauterine Growth Retardation Affects Intestinal Health of Suckling Piglets via Altering Intestinal Antioxidant Capacity, Glucose Uptake, Tight Junction, and Immune Responses X Tang,Oxidative Medicine and Cellular Longevity,2022
- Probiotics and Achyranthes bidentata Polysaccharides Improve Growth Performance via Promoting Intestinal Nutrient Utilization and Enhancing Immune Function of Weaned Pigs Gaifeng Hou,Animals,2021
- Modulation of Pectin on Mucosal Innate Immune Function in Pigs Mediated by Gut Microbiota W Wu,Microorganisms,2020
- Effects of early protein restriction on the growth performance and gut development of pigs fed diets with or without antibiotic Zhao X,animal,2019
- Effects of dietary lysozyme levels on growth performance, intestinal morphology, immunity response and microbiota community of growing pigs Lijun Zou.et al,Journal of the Science of Food and Agriculture,2018
- Effect of low dosage of chito-oligosaccharide supplementation on intestinal morphology, immune response, antioxidant capacity, and barrier function in weaned piglets Xiong X.et al,J Anim Sci.,2015
- Dietary supplementation with yeast product improves intestinal function, and serum and ileal amino acid contents in weaned piglets Xia Xiong.et al,Livestock Science,2015
- Kadar imunoglobulin A sekretori pada penderita tonsilitis kronik sebelum dan setelah tonsilektomi Indo Sakka et al,Oto Rhino Laryngologica Indonesiana ,2011
- Effects of the antimicrobial peptide cecropin AD on performance and intestinal health in weaned piglets challenged with Escherichia coli. Wu S et al,Peptides,2012
產(chǎn)品評價(jià)
平均分:
樣品類型：血清
樣品信息：其他 豬
稀釋比：沒有稀釋
產(chǎn)品評價(jià)： 標(biāo)曲準(zhǔn)確，樣本數(shù)值在標(biāo)曲范圍之內(nèi)，符合預(yù)期結(jié)果。
By 謝老師
樣品類型：細(xì)胞培養(yǎng)上清液
樣品信息：其他 豬
稀釋比：沒有稀釋
產(chǎn)品評價(jià)： 我用Pig secretory immunoglobulin A (SIgA) ELISA Kit檢測細(xì)胞培養(yǎng)上清中的SIgA含量 操作步驟很簡單 耗時(shí)短 最終測得的含量也很合理
By 劉老師
相關(guān)產(chǎn)品
相關(guān)問答
■ 常見問題解答
Q:
Could you indicate me if your kit could be valid for feces samples? Any recommendations for this type of samples?
A:
Thanks for your inquiry.
Here advice the customer to arrange preliminary experiment if you want to detect feces sample.
Below is our sample method for your reference:
Feces Dilute samples with appropriate Sample Diluent.
This can be achieved by adding 0.1g sample to appropriate Sample Diluent.
Remove particulates by centrifugation for 10 minutes at 4000x g at 2-8°C and assay immediately.Pls let me know if you have any further questions. Thank you.
Here advice the customer to arrange preliminary experiment if you want to detect feces sample.
Below is our sample method for your reference:
Feces Dilute samples with appropriate Sample Diluent.
This can be achieved by adding 0.1g sample to appropriate Sample Diluent.
Remove particulates by centrifugation for 10 minutes at 4000x g at 2-8°C and assay immediately.Pls let me know if you have any further questions. Thank you.
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