Chicken secretory immunoglobulin A,SIgA ELISA Kit

產品詳情

文獻引用 (4) 說明書下載

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

產品描述：

雞分泌型免疫球蛋白A(SIgA)酶聯免疫試劑盒（CSB-E10097Ch）為競爭法ELISA試劑盒，定量檢測血清、血漿樣本中的SIgA含量。SIgA即分泌型免疫球蛋白 A，是黏膜免疫的關鍵效應分子。它可阻止病原體附著、中和毒素，在呼吸道、胃腸道等黏膜表面發揮重要保護作用。研究聚焦其產生機制、與疾病關聯等，以探尋基于 SIgA 的疾病防治手段。試劑盒檢測范圍為0.234 μg/mL-60 μg/mL，可滿足實驗動物模型、疫苗開發等研究中黏膜免疫應答的動態監測需求，例如評估腸道病原感染后的免疫狀態、飼料添加劑對黏膜免疫的影響或疫苗免疫效果分析；為禽類免疫學、獸醫學及比較醫學領域的科研工作者提供可靠的檢測工具。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
縮寫：

SIgA
種屬：

Gallus gallus (Chicken)
樣本類型：

serum, plasma
檢測范圍：

0.234 μg/mL-60 μg/mL
靈敏度：

0.058 μg/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Others
測定原理：

quantitative
測定方法：

Competitive

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of chicken SIgA in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:200	Average %	86
	Range %	82-89
1:400	Average %	98
	Range %	92-104
1:800	Average %	97
	Range %	93-101
1:1600	Average %	88
	Range %	84-92

回收率：

The recovery of chicken SIgA spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	91	87-95
EDTA plasma (n=4)	104	98-108

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.