Mouse Pentosidine ELISA Kit
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中文名稱：小鼠戊糖素(Pentosidine)酶聯(lián)免疫試劑盒
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貨號(hào)：CSB-E09417m
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規(guī)格：96T/48T
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價(jià)格：￥3200/￥2500
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其他：
產(chǎn)品詳情
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產(chǎn)品描述：小鼠戊糖素(Pentosidine)酶聯(lián)免疫試劑盒（CSB-E09417m）為雙抗夾心法ELISA試劑盒，定量檢測(cè)血清、血漿、組織培養(yǎng)上清液、組織勻漿樣本中的Pentosidine含量。Pentosidine，即戊糖素，是一種高級(jí)糖基化終產(chǎn)物（AGEs），其生成與人體衰老、糖尿病、尿毒癥等疾病密切相關(guān)。研究顯示，乳酸菌發(fā)酵物能有效抑制戊糖素生成，具有預(yù)防或治療相關(guān)疾病的作用。其機(jī)制在于乳酸菌發(fā)酵物中的有效成分能夠減少戊糖素的生成，從而降低與AGEs相關(guān)的疾病風(fēng)險(xiǎn)。試劑盒檢測(cè)范圍為31.25 pg/mL-2000 pg/mL，廣泛應(yīng)用于糖尿病模型研究、衰老相關(guān)氧化應(yīng)激機(jī)制探索、代謝性疾病中蛋白質(zhì)修飾分析等科研領(lǐng)域，為體外實(shí)驗(yàn)提供穩(wěn)定可靠的檢測(cè)工具。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見(jiàn)產(chǎn)品說(shuō)明書。
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縮寫：Pentosidine
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種屬：Mus musculus (Mouse)
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樣本類型：serum, plasma, cell culture supernates, tissue homogenates
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檢測(cè)范圍：31.25 pg/mL-2000 pg/mL
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靈敏度：7.8 pg/mL
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反應(yīng)時(shí)間：1-5h
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樣本體積：50-100ul
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檢測(cè)波長(zhǎng)：450 nm
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研究領(lǐng)域：Others
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測(cè)定原理：quantitative
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測(cè)定方法：Sandwich
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精密度：
Intra-assay Precision (Precision within an assay): CV%<8% Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV%<10% Three samples of known concentration were tested in twenty assays to assess. -
線性度：
To assess the linearity of the assay, samples were spiked with high concentrations of mouse pentosidine in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. Sample Serum(n=4) 1:100 Average % 96 Range % 91-103 1:200 Average % 85 Range % 80-90 1:400 Average % 93 Range % 89-98 1:800 Average % 99 Range % 94-105 -
回收率：
The recovery of mouse pentosidine spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. Sample Type Average % Recovery Range Serum (n=5) 92 87-96 EDTA plasma (n=4) 94 90-98 -
標(biāo)準(zhǔn)曲線：
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. 
pg/ml OD1 OD2 Average Corrected 2000 2.401 2.462 2.432 2.251 1000 1.850 2.004 1.927 1.746 500 1.352 1.432 1.392 1.211 250 0.849 0.899 0.874 0.693 125 0.601 0.650 0.626 0.445 62.5 0.447 0.417 0.432 0.251 31.25 0.285 0.274 0.280 0.099 0 0.183 0.178 0.181 -
數(shù)據(jù)處理：
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貨期：3-5 working days
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相關(guān)問(wèn)答
■ 常見(jiàn)問(wèn)題解答
Q:
Regarding your ELISA kits CSB-E09417m, could you please advise on the following:
1) Are the standards natural material or recombinant? And if recombinant, what kind of cell or living material is it used for expression?
2) Do the standards include additive agents such as antiseptic or preservative? If they include, What is composition of additive agent?
3) Material name and concentration of Stop Solution.
4) Do these kits include hazardous substance such as azide, thimerosal, 2-ME, etc?
A:
Thanks for your inquiry.
CSB-E09417m Standard is purified Pentosidine polymer.
Preservative is porclin 300(1:3000).
Stop solution is 4N sulfuric acid；
These kits do not include hazardous substance such as azide, thimerosal, 2-ME, etc.
Pls let me know if you have any further questions. Thank you.
CSB-E09417m Standard is purified Pentosidine polymer.
Preservative is porclin 300(1:3000).
Stop solution is 4N sulfuric acid；
These kits do not include hazardous substance such as azide, thimerosal, 2-ME, etc.
Pls let me know if you have any further questions. Thank you.
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