Human soluble tumor necrosis factor receptor 2,sTNF-R2 ELISA Kit
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中文名稱：人可溶性腫瘤壞死因子受體2(sTNF-R2)酶聯(lián)免疫試劑盒
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貨號(hào)：CSB-E11266h
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規(guī)格：96T/48T
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價(jià)格：￥3600/￥2500
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其他：
產(chǎn)品詳情
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產(chǎn)品描述：人可溶性腫瘤壞死因子受體2(sTNF-R2)酶聯(lián)免疫試劑盒（CSB-E11266h）為雙抗夾心法ELISA試劑盒，定量檢測(cè)血清、血漿、組織勻漿樣本中的TNFRSF1B含量。TNFRSF1B，也稱為TNFR2或CD120b，是腫瘤壞死因子受體超家族的成員，主要表達(dá)在腫瘤微環(huán)境中的Treg細(xì)胞和多種腫瘤細(xì)胞中。研究顯示，TNFRSF1B在多種腫瘤類型的耗竭T細(xì)胞中高表達(dá)，其高表達(dá)會(huì)影響患者預(yù)后及生存周期。研究機(jī)制方面，TNFRSF1B基因高表達(dá)會(huì)抑制T細(xì)胞分泌IFN-γ，進(jìn)而影響T細(xì)胞活性和耗竭功能。試劑盒檢測(cè)范圍為31.25 pg/mL-2000 pg/mL，靈敏度為7.8 pg/mL。適用于基礎(chǔ)科研中炎癥性疾病模型評(píng)估、免疫調(diào)控通路研究以及藥物干預(yù)效果分析等場(chǎng)景。其兼容多種生物樣本的特性，為探索不同組織或體液中的sTNF - R2表達(dá)差異提供了靈活方案，可用于體外實(shí)驗(yàn)條件下的信號(hào)通路解析或生物標(biāo)志物篩選，助力科研人員深入探究TNF信號(hào)網(wǎng)絡(luò)在病理生理中的作用機(jī)制。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見(jiàn)產(chǎn)品說(shuō)明書(shū)。
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別名：CD120b ELISA Kit; p75 ELISA Kit; p75 TNF receptor ELISA Kit; p75TNFR ELISA Kit; p80 TNF alpha receptor ELISA Kit; p80 TNF-alpha receptor ELISA Kit; Soluble TNFR1B variant 1 ELISA Kit; TBP-2 ELISA Kit; TBPII ELISA Kit; TNF R II ELISA Kit; TNF R2 ELISA Kit; TNF R75 ELISA Kit; TNF-R2 ELISA Kit; TNF-RII ELISA Kit; TNFBR ELISA Kit; TNFR-II ELISA Kit; TNFR1B ELISA Kit; TNFR2 ELISA Kit; TNFR80 ELISA Kit; TNFRII ELISA Kit; Tnfrsf1b ELISA Kit; TNR1B_HUMAN ELISA Kit; Tumor necrosis factor beta receptor ELISA Kit; Tumor necrosis factor binding protein 2 ELISA Kit; Tumor necrosis factor receptor 2 ELISA Kit; Tumor necrosis factor receptor superfamily member 1B ELISA Kit; Tumor necrosis factor receptor type II ELISA Kit; Tumor necrosis factor-binding protein 2 ELISA Kit
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縮寫(xiě)：
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Uniprot No.：
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種屬：Homo sapiens (Human)
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樣本類型：serum, plasma, tissue homogenates
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檢測(cè)范圍：31.25 pg/mL-2000 pg/mL
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靈敏度：7.8 pg/mL
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反應(yīng)時(shí)間：1-5h
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樣本體積：50-100ul
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檢測(cè)波長(zhǎng)：450 nm
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研究領(lǐng)域：Signal Transduction
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測(cè)定原理：quantitative
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測(cè)定方法：Sandwich
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精密度：
Intra-assay Precision (Precision within an assay): CV%<8% Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV%<10% Three samples of known concentration were tested in twenty assays to assess. -
線性度：
To assess the linearity of the assay, samples were spiked with high concentrations of human sTNF-R2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. Sample Serum(n=4) 1:1 Average % 101 Range % 95-105 1:2 Average % 93 Range % 87-97 1:4 Average % 97 Range % 85-101 1:8 Average % 94 Range % 85-99 -
回收率：
The recovery of human sTNF-R2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. Sample Type Average % Recovery Range Serum (n=5) 100 94-104 EDTA plasma (n=4) 101 92-104 -
標(biāo)準(zhǔn)曲線：
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. 
pg/ml OD1 OD2 Average Corrected 2000 2.170 2.099 2.135 2.032 1000 1.721 1.788 1.755 1.652 500 1.272 1.243 1.258 1.155 250 0.776 0.807 0.792 0.689 125 0.487 0.501 0.494 0.391 62.5 0.306 0.322 0.314 0.211 31.25 0.209 0.213 0.211 0.108 0 0.102 0.104 0.103 -
數(shù)據(jù)處理：
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貨期：3-5 working days
引用文獻(xiàn)
- SIRT6 Ameliorates Cancer Cachexia–Associated Adipose Wasting by Suppressing TNFR2 Signalling in Mice K Xu, Y Wang, F Wang, Y Guo, Y Ren,Journal of Cachexia Sarcopenia and Muscle,2025
- Potential Role of Soluble TNF-α Receptors in Diagnosis of Patients with Chronic Kidney Disease Xun C.et al,Ann Clin Lab Sci.?,2017
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靶點(diǎn)詳情
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最新研究進(jìn)展：TNFRSF1B是一種細(xì)胞膜受體，屬于腫瘤壞死因子受體家族，它與腫瘤壞死因子（TNF）結(jié)合后，可以啟動(dòng)多種信號(hào)通路，參與炎癥反應(yīng)、免疫應(yīng)答、細(xì)胞增殖和細(xì)胞凋亡等生物學(xué)過(guò)程。近年來(lái)的研究表明，TNFRSF1B在許多疾病中都扮演著重要的角色，例如自身免疫疾病、腫瘤和炎癥性疾病等。針對(duì)TNFRSF1B的抗體和抑制劑已被廣泛研究作為治療上述疾病的候選藥物。此外，對(duì)TNFRSF1B基因的突變和多態(tài)性研究也有助于進(jìn)一步了解該受體在疾病發(fā)生和發(fā)展中的作用。
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功能：Receptor with high affinity for TNFSF2/TNF-alpha and approximately 5-fold lower affinity for homotrimeric TNFSF1/lymphotoxin-alpha. The TRAF1/TRAF2 complex recruits the apoptotic suppressors BIRC2 and BIRC3 to TNFRSF1B/TNFR2. This receptor mediates most of the metabolic effects of TNF-alpha. Isoform 2 blocks TNF-alpha-induced apoptosis, which suggests that it regulates TNF-alpha function by antagonizing its biological activity.
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基因功能參考文獻(xiàn)：
- TL1A modulated Rheumatoid arthritis-fibroblast-like synoviocytes migration and Indian hedgehog signaling pathway using TNFR2. PMID: 29748156
- Data suggest that maternal glycemic response during pregnancy is associated with lower DNA methylation of 4 CpG sites within PDE4B gene in placenta (collected after normal-weight term birth); 3 additional CpG sites are differentially methylated relative to maternal glucose response within TNFRSF1B, LDLR, and BLM genes. (PDE4B = phosphodiesterase-4B; LDLR = low density lipoprotein receptor; BLM = Bloom syndrome protein) PMID: 29752424
- serum level did not change after tonsillectomy alone but decreased significantly after steroid pulse therapy in patients with IgA nephropathy PMID: 28389814
- Elevated serum TNFR2 may be a possible marker of COPD in asymptomatic smokers and ex-smokers. PMID: 28744116
- TNFR2 promoted Adriamycin resistance in breast cancer cells by regulating the DNA damage repair. PMID: 28677724
- Serum TNFR2 is a biomarker for patients with chronic kidney disease. PMID: 28667032
- Data indicate activators of tumor necrosis factor receptor 2 (TNFR2) and a potential role for this target in immunotherapy. PMID: 27626702
- In this study, we identified a novel association between ANCA levels, TNFRSF1B genotype and decreased circulating TNFR2 levels, which may be reflective of the underlying biological mechanisms that determine clinical expression and/or response to certain therapies. PMID: 27104820
- In Han Chinese population of Hunan province, TNFRSF1B+676 gene polymorphisms are not associated with the genetic risk of rheumatoid arthritis PMID: 27640805
- Results showed that TNFR2 was upregulated in papillary thyroid carcinoma tissues, and its expression is regulated by H19. PMID: 29287713
- The TNFRSF1Brs3397 variant may play a role in modulating the risk of rheumatoid arthritis, but does not provide strong evidence of an impact of TNFRSF1B variants in determining response to anti-TNF drugs. PMID: 25850964
- atopic dermatits patients had increased TNFR2 expression on immune cells PMID: 29212072
- the blocking of tumor necrosis factor receptor 2 (TNFR2) decreased TL1A-stimulated IL-6 production by rheumatoid arthritis fibroblast-like synoviocytes. PMID: 27081759
- Data suggest that, by targeting tumor cells and immunosuppressive tumor-associated Tregs, antagonistic tumor necrosis factor receptor 2 (TNFR2) antibodies may be an effective treatment for cancers positive for TNFR2. PMID: 28096513
- p75 neurotrophin receptor (p75TNFR) was the first NGFR (nerve growth factor receptor) to be cloned and the founding member of the TNFR (tumor necrosis factor receptor) superfamily. However, data suggest that p75TNF is an atypical TNFR superfamily protein; p75TNFR forms dimers (activated by dimeric neurotrophins) that are structurally unrelated to TNFR superfamily proteins. [REVIEW] PMID: 28215307
- High plasma levels of TNFR2 and TNFR1 were associated with incident intracerebral hemorrhage. PMID: 28830973
- Serum TNFR2 levels were elevated in lupus nephritis patients versus controls. PMID: 27973968
- Voxel-based morphometry was used to analyse the associations between TNFRSF1B (rs1061624) genotypes and grey matter structure. Analysis of the TNFRSF1B SNP rs1061624 yielded a significant association with hippocampal but not with striatal volume, whereby G homozygotes were associated with increased volumes relative to A homozygotes and heterozygotes. PMID: 27528091
- Our results demonstrated that Treg frequencies and TNFR2 expression on Tregs are increased in sarcoidosis, followed by a decline during infliximab therapy, suggesting a pathophysiological role of this T cell subset. PMID: 27158798
- identified 6 known and 4 novel variations in 6 different exons of TNFR2 gene; out of these identified variations, 5 known variations were found to be significantly associated with the risk of cervical cancer; postmenopausal women having CAAGC + CTGCC haplotypes in TNFR2 gene along with HPV infection and tobacco consumption may lead to the development of cervical cancer PMID: 27145290
- LTbetaR is essential for efficient liver regeneration and cooperates with TNFRp55 in this process. Differences in survival kinetics strongly suggest distinct functions for these two cytokine receptors in liver regeneration. PMID: 26708145
- that U87-p75(NTR) cells express higher levels of Cdh-11 protein and that siRNA-mediated knockdown of Cdh-11 resulted in a significant decrease in p75(NTR)-mediated glioblastoma cell migration PMID: 26476273
- TNF-alpha/TNFR2 regulatory axis stimulates EphB2-mediated neuroregeneration via activation of NF-kappaB. PMID: 26492598
- This meta-analysis demonstrates that TNFRSF1B T allele carriers show a better response to anti-TNF therapy--{REVIEW} PMID: 26071216
- Pretransplant recipient circulating CD4+CD127lo/-TNFR2+ Treg cell is potentially a simpler alternative to Treg cell function as a pretransplant recipient immune marker for acute kidney injury. PMID: 26425877
- NGF has a role in modulating trkANGFR/p75NTR in alphaSMA-expressing conjunctival fibroblasts from human ocular cicatricial pemphigoid PMID: 26569118
- our findings implicate TNFR2 in supporting myeloid-derived suppressor cells -mediated immune suppression and metastasis in the liver PMID: 26483205
- In conclusion, we report a significant association between the TNFRSF1B p.M196R variant and the risk for psoriasis and the response to treatment with anti-TNF or anti-Il-12/Il-23. PMID: 25537528
- Plasma sTNFR2 was higher during pregnancy compared with controls. Urinary levels of sTNFR2 were higher in preeclampsia and pregnant women compared with controls. PMID: 25034210
- study suggests that TNFR2(+) Tregs play a role in promoting tumor progressive metastasis PMID: 26280204
- Recurrent point mutations and genomic gains of TNFRSF1B, encoding the tumor necrosis factor receptor TNFR2, are present in a subset of patients with mycosis fungoides and Sezary syndrome. PMID: 26258847
- Increased plasma level of sTNFRII is found to be associated with exudative age-related macular degeneration. PMID: 25363549
- ADAM17 was identified as the protease responsible for TNFR2 shedding by CD8(+) T cells, with ADAM17 and TNFR2 required in "cis" for shedding to occur. PMID: 26019295
- hTNFR2 blocks the biological activity of lymphotoxin beta. PMID: 25940088
- NRH2 enhanced the ratio of Bax/Bcl-2 by promoting the expressions of proNGF, sortilin and p75NTR, thereby inducing brain cell apoptosis. PMID: 25854576
- Only TNFR2 can induce TRAF2 degradation. PMID: 25152365
- Data show that TNF-alpha receptor TNFR2 mRNA expression was significantly increased after 6, 9 and 12 hours of poly (I:C) stimulation. PMID: 25419735
- Functional TNFR2 196 M/R polymorphism is associated with susceptibility to rheumatoid arthritis in the European population. PMID: 24777778
- Serum TNFR2 is associated with renal decline and ESRD risk in type 1 diabetes and proteinuria. PMID: 24898299
- The TNFRII nt587 G/G genotype may increase the risk of developing AS in the Chinese population. PMID: 25061744
- High TNF receptor 2 is closely associated with the loss of kidney function. PMID: 24717758
- Inflammation mediated through TNFalpha and its receptors, TNFR1 and TNFR2, may represent an important component of a comorbidity-induced inflammatory response that partially drives the pathophysiology of heart failure (HF) with preserved ejection fraction. PMID: 24923671
- TNF-TNFR2 signaling may induce RBR in naive BM-EPCs and that blocking TNF-TNFR2 signaling may prevent delayed RBR in BM-EPCs, conceivably, in bone marrow milieu in general PMID: 24711449
- Blood levels of CRP, IL-6 and TNFalpha-R2 are not associated with incident depression. PMID: 24836084
- Addition of leukemia inhibitory factor (LIF) neutralizing antibodies inhibited oligodendrocyte differentiation, indicating a crucial role of TNFR2-induced astrocyte derived LIF for oligodendrocyte maturation PMID: 24310780
- Higher plasma TNFR75 levels were associated with decreased time to first COPD exacerbatino in a prospective study. PMID: 24136332
- Our results support a role of TNFRSF1B gene variants in the response to IFX in CD patients. PMID: 24121042
- Serum TNFR2 expression levels might be a powerful prognostic factor for patients treated with the R-CHOP regimen. PMID: 23672298
- Release of nonmuscle myosin II from the cytosolic domain of tumor necrosis factor receptor 2 is required for target gene expression. PMID: 23861542
- genetic association studies in a population of women in Tunisia: Data suggest that an SNP in exon 6 of TNFR2/TNFRSF1B (rs1061622) is associated with pre-eclampsia. PMID: 23799986
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亞細(xì)胞定位：[Isoform 1]: Cell membrane; Single-pass type I membrane protein.; [Isoform 2]: Secreted.; [Tumor necrosis factor-binding protein 2]: Secreted.
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