Human adrenocorticotropic hormone (ACTH) ELISA kit

產品詳情

文獻引用 (3) 說明書下載

Intra-assay Precision (Precision within an assay): CV%<15%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<15%

Three samples of known concentration were tested in twenty assays to assess.

產品描述：

人促腎上腺皮質激素(ACTH)酶聯免疫試劑盒（CSB-E06873h）為競爭法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的ACTH含量。ACTH即促腎上腺皮質激素，由腺垂體分泌。其背景在于它可促進腎上腺皮質增生、合成與分泌腎上腺皮質激素。研究機制方面，它與腎上腺皮質細胞膜上受體結合，激活腺苷酸環化酶-cAMP-蛋白激酶系統，從而調節腎上腺皮質激素合成與分泌。試劑盒檢測范圍為12.5 pg/mL-200 pg/mL，適用于科研領域中對下丘腦 - 垂體 - 腎上腺軸功能的機制研究、疾病模型構建以及藥物干預效果的評估；為研究ACTH在生理病理過程中的動態變化提供可靠工具，尤其適用于神經內分泌學、代謝性疾病及應激反應相關的基礎研究。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
縮寫：

ACTH
種屬：

Homo sapiens (Human)
樣本類型：

serum, plasma, tissue homogenates
檢測范圍：

12.5 pg/mL-200 pg/mL
靈敏度：

12.5 pg/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Metabolism
測定原理：

quantitative
測定方法：

Competitive

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human ACTH in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	87
	Range %	82-93
1:2	Average %	94
	Range %	89-99
1:4	Average %	95
	Range %	89-99
1:8	Average %	92
	Range %	87-96

回收率：

The recovery of human ACTH spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	92	88-96
EDTA plasma (n=4)	97	95-100

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.