Mouse adrenocorticotropic hormone (ACTH) ELISA kit

產品詳情

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Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

產品描述：

小鼠促腎上腺皮質激素(ACTH)酶聯免疫試劑盒（CSB-E06874m）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿樣本中的ACTH含量。ACTH即促腎上腺皮質激素，由腺垂體分泌。其背景在于它能促進腎上腺皮質的組織增生以及皮質激素的生成和分泌。研究機制方面，它作用于腎上腺皮質細胞上的受體，激活相關信號通路，調控皮質激素合成關鍵酶的表達，進而調節機體應激等生理過程。試劑盒檢測范圍為18.75 pg/mL-1200 pg/mL，該產品適用于實驗動物模型中ACTH動態變化的分析，例如應激反應評估、腎上腺功能研究、內分泌調控機制探索以及藥物或環境因素對激素分泌影響的科研實驗，為神經內分泌領域的定量研究提供可靠工具。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
縮寫：

ACTH
種屬：

Mus musculus (Mouse)
樣本類型：

serum, plasma
檢測范圍：

18.75 pg/mL-1200 pg/mL
靈敏度：

4.68 pg/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Metabolism
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of mouse ACTH in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	92
	Range %	80-105
1:2	Average %	96
	Range %	85-104
1:4	Average %	98
	Range %	92-104
1:8	Average %	96
	Range %	82-110

回收率：

The recovery of mouse ACTH spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	84-102
EDTA plasma (n=4)	98	90-105

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.