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Human Vitamin D Receptor,VD R ELISA Kit

A micro ELISA plate --The 96-well plate has been pre-coated with an anti-human VDR antibody. This dismountable microplate can be divided into 12 x 8 strip plates.
Two vials lyophilized standard --Dilute a bottle of the standard at dilution series, read the OD values, and then draw a standard curve.
One vial Biotin-labled VDR antibody (100 x concentrate) (120 μl/bottle) --Act as the detection antibody.
One vial HRP-avidin (100 x concentrate) (120 μl/bottle) --Bind to the detection antibody and react with the TMB substrate to make the solution chromogenic.
One vial Biotin-antibody Diluent (15 ml/bottle) --Dilute the high concentration Biotin-antibody to an appropriate working solution.
One vial HRP-avidin Diluent (15 ml/bottle) --Dilute the high concentration HRP-avidin solution to an appropriate solution.
One vial Sample Diluent (50 ml/bottle)--Dilute the sample to an appropriate concentration.
One vial Wash Buffer (25 x concentrate) (20 ml/bottle) --- Wash away unbound or free substances.
One vial TMB Substrate (10 ml/bottle) --Act as the chromogenic agent. TMB interacts with HRP, eliciting the solution turns blue.
One vial Stop Solution (10 ml/bottle) --Stop the color reaction. The solution color immediately turns from blue to yellow.
Four Adhesive Strips (For 96 wells) --Cover the microplate when incubation.
An instruction manual

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To assess the linearity of the assay, samples were spiked with high concentrations of human VD R in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	90
	Range %	87-96
1:2	Average %	103
	Range %	97-105
1:4	Average %	95
	Range %	90-100
1:8	Average %	94
	Range %	88-98

The recovery of human VD R spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	89	82-94
EDTA plasma (n=4)	99	90-105

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.