Bovine Epithelial growth factor(EGF)ELISA Kit

Bovine Epithelial growth factor(EGF)ELISA Kit

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中文名稱：

牛表皮生長因子(EGF)酶聯(lián)免疫試劑盒
貨號：

CSB-E17171B
規(guī)格：

96T/48T
價格：

￥3600/￥2500
其他：

CUSABIO Wins 2024 Bioz Rapid Star? Award in the ELISA category

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牛表皮生長因子(EGF)酶聯(lián)免疫試劑盒（CSB-E17171B）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿樣本中的EGF含量。EGF（表皮生長因子）是EGF家族的創(chuàng)始成員，具有53個氨基酸殘基和三個分子內(nèi)二硫鍵。通過與細(xì)胞表面的EGFR受體結(jié)合，EGF刺激細(xì)胞生長、分化和存活，參與多種生理和病理過程，如組織再生、傷口愈合和腫瘤發(fā)生。EGF通過激活EGFR的酪氨酸激酶活性，啟動信號轉(zhuǎn)導(dǎo)級聯(lián)反應(yīng)，導(dǎo)致細(xì)胞內(nèi)鈣水平升高、糖酵解和蛋白質(zhì)合成增加，最終促進DNA合成和細(xì)胞增殖。試劑盒檢測范圍為0.156 ng/mL-10 ng/mL，該產(chǎn)品可廣泛應(yīng)用于動物生理學(xué)研究、營養(yǎng)代謝評估、疾病模型構(gòu)建等科研領(lǐng)域，為探究牛EGF在生長發(fā)育調(diào)控、免疫應(yīng)答及病理過程中的分子機制提供可靠工具。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
別名：

HOMG4, URG, beta-urogastrone|epidermal growth factor
縮寫：

EGF
種屬：

Bos taurus (Bovine)
樣本類型：

serum, plasma
檢測范圍：

0.156 ng/mL-10 ng/mL
靈敏度：

0.039 ng/mL
反應(yīng)時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領(lǐng)域：

Others
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of bovine EGF in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	94
	Range %	88-97
1:2	Average %	106
	Range %	100-109
1:4	Average %	90
	Range %	85-94
1:8	Average %	94
	Range %	90-97

回收率：

The recovery of bovine EGF spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	93	87-97
EDTA plasma (n=4)	94	89-97

標(biāo)準(zhǔn)曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
10	2.109	2.042	2.076	1.952
5	1.710	1.723	1.717	1.593
2.5	1.432	1.421	1.427	1.303
1.25	1.018	0.999	1.009	0.885
0.625	0.678	0.668	0.673	0.549
0.312	0.466	0.451	0.459	0.335
0.156	0.299	0.292	0.296	0.172
0	0.125	0.123	0.124

數(shù)據(jù)處理：

ELISA操作視頻及標(biāo)曲繪制軟件下載
貨期：

3-5 working days

Methods to improve efficacy of orally administered bioactive peptides using bovine colostrum as an exemplar RJ Playford,Plos one,2021
Bioactive immune components of anti-diarrheagenic Enterotoxigenic E. coli (ETEC) hyperimmune bovine colostrum products Sears KT.et al,Clin Vaccine Immunol.,2017

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.