Pig Epithelial growth factor,EGF ELISA Kit

Pig Epithelial growth factor,EGF ELISA Kit

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中文名稱：

豬表皮生長(zhǎng)因子(EGF)酶聯(lián)免疫試劑盒
貨號(hào)：

CSB-E06788p
規(guī)格：

96T/48T
價(jià)格：

￥3200/￥2500
其他：

CUSABIO Wins 2024 Bioz Rapid Star? Award in the ELISA category

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產(chǎn)品描述：

豬表皮生長(zhǎng)因子(EGF)酶聯(lián)免疫試劑盒（CSB-E06788p）為雙抗夾心法ELISA試劑盒，定量檢測(cè)血清、血漿、組織勻漿樣本中的EGF含量。EGF（表皮生長(zhǎng)因子）是EGF家族的創(chuàng)始成員，具有與家族成員高度相似的結(jié)構(gòu)和功能。EGF是一種低分子量多肽，最初從小鼠頜下腺中純化，后在多種人體組織中發(fā)現(xiàn)。EGF通過(guò)與細(xì)胞表面的EGFR受體結(jié)合，刺激細(xì)胞增殖、分化和存活，在傷口愈合、組織修復(fù)等方面發(fā)揮重要作用。試劑盒檢測(cè)范圍為250 pg/mL-10000 pg/mL，可廣泛應(yīng)用于動(dòng)物營(yíng)養(yǎng)學(xué)、生殖生理學(xué)、免疫學(xué)等基礎(chǔ)研究領(lǐng)域，例如探究飼料添加劑對(duì)腸道黏膜修復(fù)的影響、評(píng)估應(yīng)激狀態(tài)下EGF的動(dòng)態(tài)變化，或解析特定疾病模型中生長(zhǎng)因子調(diào)控機(jī)制本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見(jiàn)產(chǎn)品說(shuō)明書(shū)。
縮寫(xiě)：

EGF
Uniprot No.：

F1S137
種屬：

Sus scrofa (Pig)
樣本類型：

serum, plasma, tissue homogenates
檢測(cè)范圍：

250 pg/mL-10000 pg/mL
靈敏度：

125 pg/mL
反應(yīng)時(shí)間：

1-5h
樣本體積：

50-100ul
檢測(cè)波長(zhǎng)：

450 nm
研究領(lǐng)域：

Others
測(cè)定原理：

quantitative
測(cè)定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of pig EGF in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	100
	Range %	97-105
1:2	Average %	93
	Range %	90-96
1:4	Average %	102
	Range %	98-106
1:8	Average %	87
	Range %	83-90

回收率：

The recovery of pig EGF spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	86	83-89
EDTA plasma (n=4)	101	99-104

標(biāo)準(zhǔn)曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average
10000	2.647	2.687	2.667
5000	1.839	1.899	1.869
2500	1.057	1.067	1.062
1250	0.599	0.584	0.592
500	0.241	0.231	0.236
250	0.112	0.114	0.113

數(shù)據(jù)處理：

ELISA操作視頻及標(biāo)曲繪制軟件下載
貨期：

3-5 working days

Development of mADM-collagen wound dressings for mimicking native skin architecture to enhance skin wound healing X Wang,International journal of biological macromolecules,2024
Arginine and its metabolites stimulate proliferation, differentiation, and physiological function of porcine trophoblast cells through β-catenin and mTOR pathways S Li,BMC veterinary research,2024
Early biomarkers predicting outcome in a porcine model of acetaminophen intoxication: A pilot study C Thiel,Advances in clinical and experimental medicine,2022
Nano chitosan-zinc complex improves the growth performance and antioxidant capacity of the small intestine in weaned piglets M Zhang,The British journal of nutrition,2020
The Role of Autologous PRP on Deep Partial-Thickness Burn Wound Healing in Bama Pigs Sun Y,J Burn Care Res,2020

Intra-assay Precision (Precision within an assay): CV%<15%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<15%

Three samples of known concentration were tested in twenty assays to assess.