MDM2

MDM2，即鼠雙微體基因2蛋白，別名E3泛素蛋白連接酶MDM2、P53結(jié)合蛋白。它是一種由MDM2基因編碼的E3泛素連接酶，主要在細(xì)胞核質(zhì)中表達(dá)，并與多種蛋白質(zhì)的泛素化降解途徑相關(guān)。MDM2的作用機(jī)制之一是介導(dǎo)p53/TP53的泛素化，導(dǎo)致其被蛋白酶體降解，此外，MDM2還參與細(xì)胞凋亡過程，通過結(jié)合其轉(zhuǎn)錄激活域抑制p53/TP53和p73/TP73介導(dǎo)的細(xì)胞周期阻滯和凋亡。MDM2在生物學(xué)上的意義非常廣泛，它不僅參與調(diào)控p53的穩(wěn)定性和生物功能，還涉及細(xì)胞周期、細(xì)胞凋亡、DNA損傷響應(yīng)等多種細(xì)胞生理過程。MDM2與p53之間的相互作用形成了一個自動調(diào)節(jié)的負(fù)反饋循環(huán)，對維持細(xì)胞內(nèi)環(huán)境穩(wěn)定至關(guān)重要。此外，MDM2還通過調(diào)控STAT5穩(wěn)定性來控制T細(xì)胞介導(dǎo)的抗腫瘤免疫，這為癌癥免疫治療提供了新的視角和潛在靶點。

熱銷產(chǎn)品

Recombinant Mouse E3 ubiquitin-protein ligase Mdm2 (Mdm2) (CSB-EP013626MO)

驗證數(shù)據(jù)

(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.

Phospho-MDM2 (S166) Recombinant Monoclonal Antibody (CSB-RA980583A0HU)

驗證數(shù)據(jù)

IHC image of CSB-RA980583A0HU diluted at 1:50 and staining in paraffin-embedded human brain tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.57% DAB.

IHC image of CSB-RA980583A0HU diluted at 1:50 and staining in paraffin-embedded human lung cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.57% DAB.

Immunofluorescence staining of HepG2 with CSB-RA980583A0HU at 1:10, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 517-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Overlay Peak curve showing Hela cells stained with CSB-RA980583A0HU (red line) at 1:50. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1μg/1*10⁶cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated Goat Anti-rabbit IgG(H+L) at 1:200 dilution for 35min at 4℃.Control antibody (green line) was rabbit IgG (1μg/1*10⁶cells) used under the same conditions. Acquisition of >10,000 events was performed.

MDM2 Antibody

MDM2 Protein

MDM2 Antibodies

MDM2 for Homo sapiens (Human)

產(chǎn)品貨號	產(chǎn)品名稱	種屬反應(yīng)性	應(yīng)用類型
CSB-PA073718	Phospho-MDM2 (Ser166) Antibody	Human	ELISA,WB,IHC,IF
CSB-PA156585	MDM2 (Ab-166) Antibody	Human,Mouse,Rat	ELISA,WB,IHC,IF
CSB-PA729984	MDM2 Antibody	Human	ELISA,WB
CSB-PA570037	Phospho-MDM2 (Ser186/Ser188) Antibody	Human	ELISA,IF
CSB-PA891046	MDM2 Antibody	Human,Rat	ELISA,WB,IHC,IF
CSB-PA003212	MDM2 Antibody	Human,Mouse,Monkey	WB, IHC, IF, ELISA
CSB-PA005097	MDM2 Antibody	Human	WB, ELISA
CSB-PA010114	Phospho-MDM2 (S166) Antibody	Human,Mouse,Monkey	WB, IHC, IF, ELISA
CSB-PA010119	Phospho-MDM2 (S186/S188) Antibody	Human	IF, ELISA
CSB-PA010122	MDM2 Antibody	Human,Mouse	WB, IHC, IF, ELISA
CSB-PA010128	MDM2 Antibody	Human	IHC, IF, ELISA
CSB-PA973311	MDM2 Antibody	Human	ELISA,WB
CSB-PA455127	Phospho-MDM2 (Tyr394) Antibody	Human,Mouse	WB,ELISA
CSB-PA11628A0Rb	MDM2 Antibody	Human	ELISA, WB, IHC
CSB-PA11628B0Rb	MDM2 Antibody, HRP conjugated	Human	ELISA
CSB-PA11628C0Rb	MDM2 Antibody, FITC conjugated	Human
CSB-PA11628D0Rb	MDM2 Antibody, Biotin conjugated	Human	ELISA
CSB-RA980583A0HU	Phospho-MDM2 (S166) Recombinant Monoclonal Antibody	Human	ELISA, IHC, IF, FC

MDM2 Proteins

MDM2 Proteins for Mus musculus (Mouse)

產(chǎn)品貨號	產(chǎn)品名稱	來源
CSB-YP013626MO	Recombinant Mouse E3 ubiquitin-protein ligase Mdm2 (Mdm2)	Yeast
CSB-EP013626MO	Recombinant Mouse E3 ubiquitin-protein ligase Mdm2 (Mdm2)	E.coli
CSB-BP013626MO CSB-MP013626MO CSB-EP013626MO-B	Recombinant Mouse E3 ubiquitin-protein ligase Mdm2 (Mdm2)	Baculovirus Mammalian cell In Vivo Biotinylation in E.coli

MDM2 Proteins for Equus caballus (Horse)

產(chǎn)品貨號	產(chǎn)品名稱	來源
CSB-YP013626HO CSB-EP013626HO CSB-BP013626HO CSB-MP013626HO CSB-EP013626HO-B	Recombinant Horse E3 ubiquitin-protein ligase Mdm2 (MDM2)	Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli

MDM2 Proteins for Canis familiaris (Dog) (Canis lupus familiaris)

產(chǎn)品貨號	產(chǎn)品名稱	來源
CSB-YP013626DO CSB-EP013626DO CSB-BP013626DO CSB-MP013626DO CSB-EP013626DO-B	Recombinant Dog E3 ubiquitin-protein ligase Mdm2 (MDM2)	Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli

MDM2 Proteins for Xenopus laevis (African clawed frog)

產(chǎn)品貨號	產(chǎn)品名稱	來源
CSB-YP013626XBE CSB-EP013626XBE CSB-BP013626XBE CSB-MP013626XBE CSB-EP013626XBE-B	Recombinant Xenopus laevis E3 ubiquitin-protein ligase Mdm2 (mdm2)	Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli

MDM2 Proteins for Danio rerio (Zebrafish) (Brachydanio rerio)

產(chǎn)品貨號	產(chǎn)品名稱	來源
CSB-YP013626DIL CSB-EP013626DIL CSB-BP013626DIL CSB-MP013626DIL CSB-EP013626DIL-B	Recombinant Danio rerio E3 ubiquitin-protein ligase Mdm2 (mdm2)	Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli

MDM2 Proteins for Mesocricetus auratus (Golden hamster)

產(chǎn)品貨號	產(chǎn)品名稱	來源
CSB-YP720145MRG CSB-EP720145MRG CSB-BP720145MRG CSB-MP720145MRG CSB-EP720145MRG-B	Recombinant Mesocricetus auratus E3 ubiquitin-protein ligase Mdm2 (MDM2)	Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli

MDM2 Proteins for Felis catus (Cat) (Felis silvestris catus)

產(chǎn)品貨號	產(chǎn)品名稱	來源
CSB-YP770328CA CSB-EP770328CA CSB-BP770328CA CSB-MP770328CA CSB-EP770328CA-B	Recombinant Cat E3 ubiquitin-protein ligase Mdm2 (MDM2)	Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli