Phospho-MDM2 (S166) Recombinant Monoclonal Antibody

說明書

中文名稱：

磷酸化-MDM2 (S166)重組抗體
貨號：

CSB-RA980583A0HU
規格：

￥1320
圖片：
IHC image of CSB-RA980583A0HU diluted at 1:50 and staining in paraffin-embedded human brain tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.57% DAB.

IHC image of CSB-RA980583A0HU diluted at 1:50 and staining in paraffin-embedded human lung cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.57% DAB.

Immunofluorescence staining of HepG2 with CSB-RA980583A0HU at 1:10, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 517-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Overlay Peak curve showing Hela cells stained with CSB-RA980583A0HU (red line) at 1:50. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1μg/1*10⁶cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated Goat Anti-rabbit IgG(H+L) at 1:200 dilution for 35min at 4℃.Control antibody (green line) was rabbit IgG (1μg/1*10⁶cells) used under the same conditions. Acquisition of >10,000 events was performed.
其他：

產品詳情
產品評價
相關產品
靶點詳情

產品詳情

產品描述：

CSB-RA980583A0HU Phospho-MDM2 (S166) Recombinant Monoclonal Antibody是一款特異性識別MDM2蛋白第166位絲氨酸磷酸化修飾的高靈敏度檢測工具。MDM2作為p53信號通路的關鍵調控因子，其S166位點的磷酸化可促進核轉位并增強E3泛素連接酶活性，在腫瘤發生、DNA損傷應答及細胞周期調控中發揮重要作用。該抗體經ELISA、免疫組化（IHC）、免疫熒光（IF）和流式細胞術（FC）多平臺驗證，在推薦稀釋比例（IHC/IF/FC 1:50-1:200）下展現出優異的抗原結合特異性與低背景信號，能清晰顯示磷酸化MDM2在細胞核內的定位特征及動態修飾水平。適用于探索腫瘤微環境中MDM2-p53調控網絡的分子機制，評估DNA損傷藥物或激酶抑制劑對MDM2磷酸化的影響，以及通過多色流式技術分析細胞周期依賴性的修飾變化，為癌癥生物學研究和靶向治療開發提供可靠檢測方案。
Uniprot No.：

Q00987
基因名：

MDM2
別名：

E3 ubiquitin-protein ligase Mdm2 (EC 2.3.2.27) (Double minute 2 protein) (Hdm2) (Oncoprotein Mdm2) (RING-type E3 ubiquitin transferase Mdm2) (p53-binding protein Mdm2), MDM2
反應種屬：

Human
免疫原：

A synthesized peptide derived from Human MDM2
免疫原種屬：

Homo sapiens (Human)
標記方式：

Non-conjugated
克隆類型：

Monoclonal
抗體亞型：

Rabbit IgG
純化方式：

Affinity-chromatography
克隆號：

14F8
濃度：

It differs from different batches. Please contact us to confirm it.
保存緩沖液：

Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.
產品提供形式：

Liquid
應用范圍：

ELISA, IHC, IF, FC

推薦稀釋比：

Application	Recommended Dilution
IHC	1:50-1:200
IF	1:50-1:200
FC	1:50-1:200

Protocols：

ELISA Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol
Flow cytometry (FC) Protocol
儲存條件：

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
貨期：

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
用途：

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產品評價

靶點詳情

功能：

E3 ubiquitin-protein ligase that mediates ubiquitination of p53/TP53, leading to its degradation by the proteasome. Inhibits p53/TP53- and p73/TP73-mediated cell cycle arrest and apoptosis by binding its transcriptional activation domain. Also acts as a ubiquitin ligase E3 toward itself and ARRB1. Permits the nuclear export of p53/TP53. Promotes proteasome-dependent ubiquitin-independent degradation of retinoblastoma RB1 protein. Inhibits DAXX-mediated apoptosis by inducing its ubiquitination and degradation. Component of the TRIM28/KAP1-MDM2-p53/TP53 complex involved in stabilizing p53/TP53. Also component of the TRIM28/KAP1-ERBB4-MDM2 complex which links growth factor and DNA damage response pathways. Mediates ubiquitination and subsequent proteasome degradation of DYRK2 in nucleus. Ubiquitinates IGF1R and SNAI1 and promotes them to proteasomal degradation. Ubiquitinates DCX, leading to DCX degradation and reduction of the dendritic spine density of olfactory bulb granule cells. Ubiquitinates DLG4, leading to proteasomal degradation of DLG4 which is required for AMPA receptor endocytosis. Negatively regulates NDUFS1, leading to decreased mitochondrial respiration, marked oxidative stress, and commitment to the mitochondrial pathway of apoptosis. Binds NDUFS1 leading to its cytosolic retention rather than mitochondrial localization resulting in decreased supercomplex assembly (interactions between complex I and complex III), decreased complex I activity, ROS production, and apoptosis.
基因功能參考文獻：
1. Meta-analysis suggested that MDM2 SNP309 polymorphism increased the risk of endometrial cancer significantly, especially in endometrioid and Type I endometrial cancer, indicating MDM2 could serve as a potential diagnostic factor marker for endometrial cancer. PMID: 30544386
2. The Numb-Mdm2 interaction represents a fuzzy complex mediated by a short Numb sequence encompassing its alternatively spliced exon 3 (Ex3), which is necessary and sufficient to inhibit Mdm2 and prevent p53 degradation. PMID: 29269425
3. It has been shown that MDM2 and MDMX are targetable vulnerabilities within TP53-wild-type T-cell lymphomas. PMID: 29789628
4. MDM2 down-regulation attenuates the senescence-associated secretory phenotype. PMID: 29402901
5. First evidence that DNA induction of MDM2 promotes proliferation of human renal mesangial cells and alters peripheral B cells subsets in pediatric systemic lupus erythematosus. PMID: 29324237
6. The genotypes of MDM2 SNP309 may allow forr early detection of and predictor for colorectal cancer risk, especially among smokers and non-alcohol drinkers, but not for prognosis. PMID: 30194081
7. Our study showed that miR-145 suppressed MDM2 expression, which subsequently influenced the p53-related cell growth pattern in pterygial epithelium. The regulatory miR-145/MDM2-p53 loop can serve as a potential target for treatment of pterygium. PMID: 29360447
8. In contrast to other deubiquitinating enzymes (DUBs) that have been previously implicated in the regulation of Mdm2 protein stability, USP48 did not induce Mdm2 stabilization by significantly reducing Mdm2 ubiquitination levels. PMID: 28233861
9. MDM2 rs937283 A > G variant is associated with lung and gastric cancer. PMID: 29777315
10. No associations were found between MDM2 SNP309 and either of two FSH/LH groups. PMID: 29957069
11. MDM2 promoter variants play a role in determining the risk of recurrence of squamous cell carcinoma of oropharynx PMID: 28045062
12. In silico molecular docking and dynamics studies with MDM2-p53 protein revealed that HTMF was more potent compound that could inhibit the binding of MDM2 with p53 and, therefore, could trigger apoptosis in cancer cell. PMID: 29734849
13. Our findings suggested that RBM38 may be a core contributor in stabilizing the p53-mdm2 loop function to prevent hepatocellular carcinoma (HCC) and a potential novel target to provide a therapeutic strategy for HCC by inhibiting mdm2 and rescuing p53 from inactivation. PMID: 30176896
14. As shown in human MDM2-ALT1-expressing p53 null transgenic mice, MDM2-ALT1 can direct rhabdomyosarcoma (RMS) tumor formation recapitulating many of the histological and immunohistochemical features of fusion-negative RMS. PMID: 28892044
15. We demonstrate that extraskeletal osteosarcoma (ESOS) may include at least two small subsets: an MDM2-amplified deep soft-tissue ESOS and an H3K27me3-deficient organ-based ESOS PMID: 29489027
16. miR-518 acted as a new tumor suppressor by targeting MDM2 gene and trigger apoptosis in vivo and in vitro. PMID: 29793321
17. Overexpression of miR-641 decreased the expression of MDM2 and increased the expression of p53 in lung cancer cells. PMID: 28800790
18. The MDM2 T309G polymorphism GG genotype and the TG+GG combination may be risk factors for breast cancer in our Turkish population. PMID: 29699057
19. Human blastocyst-secreted miR-661 reduces endometrial epithelial cell adhesion via downregulation of MDM2, regulating endometrial-blastocyst adhesion, and implantation. PMID: 28847363
20. MDM2 is associated with giant cell tumor of bone recurrence, which might serve as biomarker for giant cell tumor of bone recurrence PMID: 29651441
21. The ID genotype of the MDM2 I/D polymorphism was associated with a lower risk of SLE. There was no association between MDM2 T309G polymorphism and SLE. PMID: 28676527
22. The present study demonstrated that the oncostatic effects of melatonin on SGC-7901 GC cells are mediated via the blockade of the AKT/MDM2 intracellular pathway. PMID: 29484412
23. Nongenotoxic p53 activation suppresses mTOR activity. Moreover, p53 reactivation via RG7388, a second-generation MDM2 inhibitor, strongly enhances the in vivo antitumor activity of temsirolimus PMID: 28821555
24. Overview of the connections between p53-MDM2 axis and human aging disorders and aging-related pathways (review). PMID: 29192902
25. The Role of MDM2 in genome stability/instability and DNA repair is reviewed. PMID: 29065514
26. Notch1 signaling is an essential downstream pathway of MDM2 in mediating high glucose-induced mitotic catastrophe in podocytes. PMID: 28643424
27. our data confirmed the individual susceptibility to BC resulting from polymorphic markers of DNA repair genes (XRCC1), apoptosis genes (TP53), as well as of apoptosis inhibition genes (MDM2). PMID: 29132330
28. In multivariate analysis, MDM2/MDM4 and EGFR alterations correlated with time-to-treatment failure (TTF)..Some patients with MDM2 family amplification or EGFR aberrations had poor clinical outcome and significantly increased rate of tumor growth after single-agent checkpoint (PD-1/PD-L1) inhibitors PMID: 28351930
29. Results demonstrate that Mdm2 is extremely important in breast cancer metastases to the lung. Specifically, Mdm2 is important to promote cancer invasiveness via cell migration, angiogenesis, and intravasation. PMID: 28784612
30. GG genotype of MDM2 re2279744 showed a statistically significantly increased risk of developing endometrial cancer risk in a Chinese Han population. PMID: 29096752
31. GATA4 was a transcription factor that activated mouse double minute 2 homolog (MDM2) and B cell lymphoma 2 (BCL2) expression in ALL cells. PMID: 28849107
32. Findings of the study confirm that L-THP resulted in p53 independent apoptosis via down-regulating XIAP protein by inhibiting MDM2 associated with proteasome-dependent pathway and increased sensitivity of EU-4 cells against doxorubicin. PMID: 28721806
33. MDM2 promoter SNP55 (rs2870820) affects risk of colon cancer but not breast-, lung-, or prostate cancer. PMID: 27624283
34. Importantly, these results imply that the Zika virus capsid protein interacts with mouse double-minute-2 homolog (MDM2), which is involved in the P53-mediated apoptosis pathway, activating the death of infected neural cells. PMID: 28775961
35. The expression levels of Bcl11a and Mdm2, Pten in B-ALL patients with CR were decreased significantly when compared with the healthy control (P < 0.05). PMID: 28544358
36. A near-native models of the p53-MDM2 complex have been presented. PMID: 27905468
37. the MDM2 rs937283 polymorphism is a novel functional SNP both in vitro and in vivo as well as a biomarker for poor prognosis in retinoblastoma PMID: 27506496
38. Markov models of the apo-MDM2 lid region reveal diffuse yet two-state binding dynamics and receptor poses for computational docking. PMID: 27538695
39. The nucleolar protein CSIG is a novel and crucial regulator of the MDM2-p53 pathway. We demonstrate that CSIG translocates from the nucleolus to the nucleoplasm in response to nucleolar stress. Moreover, knockdown of CSIG attenuates the induction of p53 and abrogates G1 phase arrest in response to nucleolar stress. PMID: 27811966
40. Data indicate that murine double minute 2 protein (MDMX) expression may serve as an independent unfavorable prognostic factor for non-small cell lung cancer (NSCLC). patient outcome, which in turn may at least partly be due to the ability of the MDMX protein to regulate the proliferative capacity and chemosensitivity of NSCLC cells. PMID: 28567715
41. our data suggest that estrogen provokes signals to increase MDM2 expression and this estrogen stimulated MDM2 promotes signal transduction for increasing phosphorylation of Rb. PMID: 28615518
42. The MDM2 Del1518 polymorphism (rs3730485) was associated with breast cancer susceptibility, particularly in menopausal patients with breast cancer who reported tobacco consumption, pregnancy loss, obesity and high glucose levels in Mexican population. PMID: 28667029
43. study showed that UVB induces alternative splicing of hdm2 by increasing the expression and the binding of hnRNP A1 to hdm2 full-length mRNA PMID: 26757361
44. in colon cancer cell migration, activin utilizes NFkB to induce MDM2 activity leading to the degradation of p21 in a PI3K dependent mechanism PMID: 28418896
45. Author demonstrated that LRRK2 increases the expression of p53 and p21 by increasing the Mdm2 phosphorylation in response to DNA damage. Loss-of-function in LRRK2 has the opposite effect to that of LRRK2. PMID: 28973420
46. Relevant SNPs in DNA repair (ERCC1 and ERCC5) and apoptosis (MDM2 and TP53) genes might influence the severity of radiation-related side-effects in HNSCC patients. Prospective clinical SNP-based validation studies are needed on these bases PMID: 28351583
47. this is the first documentation of MDM2 amplification in laryngeal/hypopharyngeal well-differentiated liposarcomas PMID: 27492446
48. The MDM2 309GG genotype was associated with higher risk of preeclampsia. PMID: 28508227
49. A meta-analysis of case-control studies found that MDM2 rs2279744 (SNP309) and rs117039649 (SNP285) were both associated with the risk of gynecological cancers. Subgroup analysis showed that rs2279744 (SNP309) was associated with the risk of gynecological cancers in Caucasian and Asian according to the ethnicity and cancer type, especially for endometrial cancer. PMID: 29480845
50. we here show that subgroups of SDCs display genomic amplifications of MDM2 and/or CDK4, partly in association with TP53 mutations and rearrangement/amplification of HMGA2. PMID: 27662657
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相關疾病：

Seems to be amplified in certain tumors (including soft tissue sarcomas, osteosarcomas and gliomas). A higher frequency of splice variants lacking p53 binding domain sequences was found in late-stage and high-grade ovarian and bladder carcinomas. Four of the splice variants show loss of p53 binding.
亞細胞定位：

Nucleus, nucleoplasm. Cytoplasm. Nucleus, nucleolus. Nucleus. Note=Expressed predominantly in the nucleoplasm. Interaction with ARF(P14) results in the localization of both proteins to the nucleolus. The nucleolar localization signals in both ARF(P14) and MDM2 may be necessary to allow efficient nucleolar localization of both proteins. Colocalizes with RASSF1 isoform A in the nucleus.
蛋白家族：

MDM2/MDM4 family
組織特異性：

Ubiquitous. Isoform Mdm2-A, isoform Mdm2-B, isoform Mdm2-C, isoform Mdm2-D, isoform Mdm2-E, isoform Mdm2-F and isoform Mdm2-G are observed in a range of cancers but absent in normal tissues.
數據庫鏈接：

HGNC: 6973

OMIM: 164785

KEGG: hsa:4193

STRING: 9606.ENSP00000417281

UniGene: Hs.484551

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