Rat Apolipoprotein M(APOM) ELISA kit

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產品詳情

產品描述：

大鼠載脂蛋白M(APOM)酶聯免疫試劑盒（CSB-EL001947RA）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的APOM含量。APOM為一種載脂蛋白，在脂代謝、炎癥反應等生理過程中發揮重要作用。研究機制主要聚焦于其與高密度脂蛋白（HDL）結合，參與膽固醇逆向轉運等過程，還可能和動脈粥樣硬化、神經退行性疾病等發病機制存在關聯，是潛在治療靶點。試劑盒檢測范圍為1.56 ng/mL-100 ng/mL，該產品適用于探索APOM在動脈粥樣硬化、糖尿病并發癥等病理過程中的生物學功能，也可用于評估藥物干預或基因調控對APOM表達水平的影響。為研究脂質代謝機制、建立心血管疾病動物模型提供可靠工具。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
別名：

ApomApolipoprotein M ELISA kit; Apo-M ELISA kit; ApoM ELISA kit; Protein Px ELISA kit
縮寫：

APOM
Uniprot No.：

P14630
種屬：

Rattus norvegicus (Rat)
樣本類型：

serum, plasma, tissue homogenates
檢測范圍：

1.56 ng/mL-100 ng/mL
靈敏度：

0.39 ng/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Signal Transduction
測定原理：

quantitative
測定方法：

Sandwich

精密度：

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of rat APOM in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:200	Average %	96
	Range %	92-100
1:400	Average %	90
	Range %	85-96
1:800	Average %	90
	Range %	88-94
1:1600	Average %	100
	Range %	96-105

回收率：

The recovery of rat APOM spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	91	87-94
EDTA plasma (n=4)	95	90-99

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

功能：

Probably involved in lipid transport. Can bind sphingosine-1-phosphate, myristic acid, palmitic acid and stearic acid, retinol, all-trans-retinoic acid and 9-cis-retinoic acid.
基因功能參考文獻：
1. Hyperglycemia-induced downregulation of apolipoprotein M expression is not via the hexosamine pathway. PMID: 26377577
2. ApoM occurrence in urine may be considered to be a biomarker of acute renal injury. PMID: 23953565
3. ApoM levels and expression in rats rendered hyperglycemic by short-term glucose infusion, was investigated. PMID: 17556016
亞細胞定位：

Secreted. Note=Associated with HDL.
蛋白家族：

Calycin superfamily, Lipocalin family
組織特異性：

Expressed by the liver; secreted in plasma.
數據庫鏈接：

KEGG: rno:55939

STRING: 10116.ENSRNOP00000001126

UniGene: Rn.262