Recombinant Rat Eukaryotic translation initiation factor 2-alpha kinase 3 (Eif2ak3), partial

中文名稱：

大鼠Eif2ak3重組蛋白
貨號：

CSB-YP007512RA
說明書：

MSDS Datasheet
規格：
來源：

Yeast
其他：

中文名稱：

大鼠Eif2ak3重組蛋白
貨號：

CSB-EP007512RA
說明書：

MSDS Datasheet
規格：
來源：

E.coli
其他：

中文名稱：

大鼠Eif2ak3重組蛋白
貨號：

CSB-EP007512RA-B
說明書：

MSDS Datasheet
規格：
來源：

E.coli
共軛：

Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
其他：

中文名稱：

大鼠Eif2ak3重組蛋白
貨號：

CSB-BP007512RA
說明書：

MSDS Datasheet
規格：
來源：

Baculovirus
其他：

中文名稱：

大鼠Eif2ak3重組蛋白
貨號：

CSB-MP007512RA
說明書：

MSDS Datasheet
規格：
來源：

Mammalian cell
其他：

產品詳情
產品評價
靶點詳情

產品詳情

純度：

>85% (SDS-PAGE)
基因名：

Eif2ak3
Uniprot No.：

Q9Z1Z1
別名：

Eif2ak3; Pek; PerkEukaryotic translation initiation factor 2-alpha kinase 3; EC 2.7.11.1; PRKR-like endoplasmic reticulum kinase; Pancreatic eIF2-alpha kinase
種屬：

Rattus norvegicus (Rat)
蛋白長度：

Partial
蛋白標簽：

Tag?type?will?be?determined?during?the?manufacturing?process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
產品提供形式：

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
復溶：

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
儲存條件：

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
保質期：

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
貨期：

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
注意事項：

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet ：

Please contact us to get it.

產品評價

靶點詳情

功能：

Metabolic-stress sensing protein kinase that phosphorylates the alpha subunit of eukaryotic translation initiation factor 2 (EIF2S1/eIF-2-alpha) in response to various stress conditions. Key activator of the integrated stress response (ISR) required for adaptation to various stress, such as unfolded protein response (UPR) and low amino acid availability. EIF2S1/eIF-2-alpha phosphorylation in response to stress converts EIF2S1/eIF-2-alpha in a global protein synthesis inhibitor, leading to a global attenuation of cap-dependent translation, while concomitantly initiating the preferential translation of ISR-specific mRNAs, such as the transcriptional activators ATF4 and QRICH1, and hence allowing ATF4- and QRICH1-mediated reprogramming. Serves as a critical effector of unfolded protein response (UPR)-induced G1 growth arrest due to the loss of cyclin-D1 (CCND1). Involved in control of mitochondrial morphology and function.
基因功能參考文獻：
1. These results suggest that the administration of Pien Tze Huang ameliorated the severity of alcoholic and high-fat diet rats possibly by inhibiting the Hcy-induced PERK/eIF2alpha pathway. PMID: 30005895
2. AS-IV may attenuate inflammatory responses by disrupting the crosstalk between autophagy and the PERK-eIF2alpha pathway and may be an ideal agent for treating inflammatory pulmonary diseases. PMID: 28641278
3. Pre-ischemia melatonin treatment alleviated acute neuronal injury after ischemic stroke by inhibiting endoplasmic reticulum stress-dependent autophagy via PERK and IRE1 signalings PMID: 28178380
4. these results confirmed that ER stress-mediated apoptosis contributes to the protection effects of naringenin against H/R injury, which is potentially involved in ATF6, IRE1alpha and PERK signaling activation. PMID: 27785700
5. Cirrhotic rats with diabetes showed higher intrahepatic ET-1 vasoresponsiveness than normoglycemic cirrhotic rats. This effect may be related, at least in part, to intrahepatic ETA R receptor and pERK over-expression. PMID: 24636620
6. Suggest role for PERK in modulating fluoride induced bone formation and bone resorption. PMID: 25132241
7. PERK was activated in reactive oxygen species-mediated endoplasmic reticulum stress-induced apoptosis in a rat model of diabetic cardiomyopathy. PERK deletion in myocytes showed a protective effect against apoptosis induced by high-glucose incubation. PMID: 24180212
8. Autophagy induced by (125)I radiation was involved in endoplasmic reticulum stress and mainly dependent on PERK-eIF2alpha pathway. PMID: 24223705
9. interplay between calcineurin and PERK regulates beta-cell Ca(2+) signaling and insulin secretion, and that loss of this interaction may have profound implications in insulin secretion defects associated with diabetes. PMID: 24114838
10. The number of PERK- and CHOP-positive cells is significantly higher in diabetic and high glucose-exposed retina cultures than in normal retinas. PMID: 21767604
11. Activation of the PERK/eIF2a signaling pathway contributes to the occurrence and development of hepatocyte apoptosis in alcoholic liver injury. PMID: 21059295
12. eukaryotic translation initiation factor 2 alpha kinase 3 (PERK) is activated following oxygen-glucose deprivation of rat cortical cells PMID: 21525936
13. A decrease in the ATP/energy status in response to a decrease in glucose results in SERCA pump inhibition, the efflux of Ca2+ from the ER, and the activation of PERK, which plays an important role in both pancreatic beta-cell function and survival. PMID: 21193559
14. Endoplasmic reticulum stress blunts cytokine-triggered activation of NF-kappaB, in part through PERK- and IRE1-mediated preferential induction of C/EBPbeta. PMID: 19875812
15. Translation mediated by the internal ribosome entry site of the cat-1 mRNA is regulated by glucose availability in a PERK kinase-dependent manner PMID: 11781318
16. Renal ischemia and reperfusion activates the eIF 2 alpha kinase PERK. PMID: 15936177
17. force-induced apoptosis depends on a novel function of PERK that occurs in addition to its canonical role in the unfolded protein response PMID: 18550523
18. Dysregulation of endoplasmic reticulum chaperone/folding enzymes ERp72 and GRP78/BiP occurred early after ablation of PERK function PMID: 19732428
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亞細胞定位：

Endoplasmic reticulum membrane; Single-pass type I membrane protein.
蛋白家族：

Protein kinase superfamily, Ser/Thr protein kinase family, GCN2 subfamily
組織特異性：

Ubiquitous.
數據庫鏈接：

KEGG: rno:29702

STRING: 10116.ENSRNOP00000008451

UniGene: Rn.24897