Recombinant Human Histidine--tRNA ligase, cytoplasmic (HARS1)

中文名稱：

人HARS1重組蛋白
貨號：

CSB-YP010137HU
說明書：

MSDS Datasheet
規格：
來源：

Yeast
其他：

中文名稱：

人HARS1重組蛋白
貨號：

CSB-EP010137HU
說明書：

MSDS Datasheet
規格：
來源：

E.coli
其他：

中文名稱：

人HARS1重組蛋白
貨號：

CSB-EP010137HU-B
說明書：

MSDS Datasheet
規格：
來源：

E.coli
共軛：

Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
其他：

中文名稱：

人HARS1重組蛋白
貨號：

CSB-MP010137HU
說明書：

MSDS Datasheet
規格：
來源：

Mammalian cell
其他：

產品詳情
產品評價
相關產品
靶點詳情

產品詳情

純度：

>85% (SDS-PAGE)
基因名：

HARS1
Uniprot No.：

P12081
別名：

cytoplasmic; EC 6.1.1.21 ; FLJ20491 ; HARS; HisRS; Histidine tRNA ligase, cytoplasmic; histidine translase; Histidine tRNA ligase; Histidine--tRNA ligase; Histidyl tRNA synthetase ; Histidyl-tRNA synthetase; HRS ; Human histidyl tRNA synthetase homolog (HO3) mRNA complete cds; SYHC_HUMAN; USH3B
種屬：

Homo sapiens (Human)
蛋白長度：

Full Length of Mature Protein
表達區域：

2-509
氨基酸序列

AERAALEEL VKLQGERVRG LKQQKASAEL IEEEVAKLLK LKAQLGPDES KQKFVLKTPK GTRDYSPRQM AVREKVFDVI IRCFKRHGAE VIDTPVFELK ETLMGKYGED SKLIYDLKDQ GGELLSLRYD LTVPFARYLA MNKLTNIKRY HIAKVYRRDN PAMTRGRYRE FYQCDFDIAG NFDPMIPDAE CLKIMCEILS SLQIGDFLVK VNDRRILDGM FAICGVSDSK FRTICSSVDK LDKVSWEEVK NEMVGEKGLA PEVADRIGDY VQQHGGVSLV EQLLQDPKLS QNKQALEGLG DLKLLFEYLT LFGIDDKISF DLSLARGLDY YTGVIYEAVL LQTPAQAGEE PLGVGSVAAG GRYDGLVGMF DPKGRKVPCV GLSIGVERIF SIVEQRLEAL EEKIRTTETQ VLVASAQKKL LEERLKLVSE LWDAGIKAEL LYKKNPKLLN QLQYCEEAGI PLVAIIGEQE LKDGVIKLRS VTSREEVDVR REDLVEEIKR RTGQPLCIC
蛋白標簽：

Tag?type?will?be?determined?during?the?manufacturing?process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
產品提供形式：

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
復溶：

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
儲存條件：

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
保質期：

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
貨期：

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
注意事項：

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet ：

Please contact us to get it.

產品評價

靶點詳情

功能：

Catalyzes the ATP-dependent ligation of histidine to the 3'-end of its cognate tRNA, via the formation of an aminoacyl-adenylate intermediate (His-AMP). Plays a role in axon guidance.
基因功能參考文獻：
1. This study suggests that the human HisRS genes, while descending from a common ancestor with dual function for both types of tRNA(His), have acquired highly specialized tRNA recognition properties through evolution. PMID: 28321488
2. Despite the similar kinetics, differential scanning fluorimetry revealed that Y454S is less thermally stable than Wild Type HARS, and cells from Y454S patients grown at elevated temperatures demonstrate diminished levels of protein synthesis compared to those of Wild Type cells. The thermal sensitivity associated with the Y454S mutation represents a biochemical basis for understanding Usher Syndrome Type IIIB. PMID: 28632987
3. Loss of function mutations in histidyl-tRNA synthetase cause a spectrum of inherited peripheral neuropathies PMID: 26072516
4. Data suggest that by comparing human and trypanosomatid histidyl-tRNA synthetases (HisRS) may provide opportunities for developing specific inhibitors of Trypanosoma brucei HisRS. PMID: 25151410
5. Secreted histidyl-tRNA synthetase splice variants elaborate major epitopes for autoantibodies in inflammatory myositis. PMID: 24898250
6. Data indicate that higher anti-Jo1 levels were associated with disease severity in antisynthetase syndrome (ASS) patients. PMID: 24286268
7. Non-Jo-1 anti-synAb positive patients have decreased survival compared with Jo-1 patients. PMID: 23422076
8. Findings suggest that histidyl-tRNA synthetase (HARS) is associated with axonal peripheral neuropathy. PMID: 22930593
9. The crystal structure of a novel splice variant of a tRNA synthetase lacking the entire catalytic domain. PMID: 22958643
10. Although anti-Jo1 positive patients with antisynthetase syndrome share features of patients with anti-PL7/PL12 antibody, they exhibit many differences regarding clinical phenotype and long-term outcome. PMID: 22326685
11. Study identified sequence variants in the known disease-causing genes SLC6A3 and FLVCR1, and present evidence to strongly support the pathogenicity of variants identified in TUBGCP6, BRAT1, SNIP1, CRADD, and HARS. PMID: 22279524
12. genomic organization of the HARS locus and mapping of transcripts originating from a bi-directional promoter controlling the differential expression of these gene PMID: 12056811
13. Demonstrating histidyl-tRNA synthetase (Jo-1)-specific T cell responses represents a key step in establishing the hypothesis that Jo-1 drives T cell-mediated autoimmunity in Jo-1+ polymyositis. PMID: 12471150
14. the TSG101 interaction with HRS is a crucial step in endocytic down-regulation of mitogenic signaling and this interaction may have a role in linking the functions of early and late endosomes PMID: 12802020
15. A proteolytically sensitive conformation of HisRS exists in the lung, the target tissue associated with this autoantibody response. PMID: 17665459
16. clinical and prognostic profiles of 45 patients displaying anti-histidyl-tRNA synthetase autoantibodies were determined PMID: 17785330
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相關疾病：

Usher syndrome 3B (USH3B); Charcot-Marie-Tooth disease 2W (CMT2W)
亞細胞定位：

Cytoplasm.
蛋白家族：

Class-II aminoacyl-tRNA synthetase family
組織特異性：

Brain, heart, liver and kidney.
數據庫鏈接：

HGNC: 4816

OMIM: 142810

KEGG: hsa:3035

STRING: 9606.ENSP00000425634

UniGene: Hs.528050