HARS Antibody

說明書

中文名稱：

HARS兔多克隆抗體
貨號：

CSB-PA002910
規格：

￥1090
圖片：
Western Blot analysis of COLO205 cells using HisRS Polyclonal Antibody
其他：

產品詳情
產品評價
相關產品
靶點詳情

產品詳情

Uniprot No.：

P12081
基因名：

HARS
別名：

cytoplasmic antibody; EC 6.1.1.21 antibody; FLJ20491 antibody; HARS antibody; HisRS antibody; Histidine tRNA ligase, cytoplasmic antibody; histidine translase antibody; Histidine tRNA ligase antibody; Histidine--tRNA ligase antibody; Histidyl tRNA synthetase antibody; Histidyl-tRNA synthetase antibody; HRS antibody; Human histidyl tRNA synthetase homolog (HO3) mRNA complete cds antibody; SYHC_HUMAN antibody; USH3B antibody
宿主：

Rabbit
反應種屬：

Human,Mouse
免疫原：

Synthesized peptide derived from the C-terminal region of Human HisRS.
免疫原種屬：

Homo sapiens (Human)
標記方式：

Non-conjugated
抗體亞型：

IgG
純化方式：

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
濃度：

It differs from different batches. Please contact us to confirm it.
保存緩沖液：

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
產品提供形式：

Liquid
應用范圍：

WB, IHC, IF, ELISA

推薦稀釋比：

Application	Recommended Dilution
WB	1:500-1:2000
IHC	1:100-1:300
IF	1:200-1:1000
ELISA	1:20000

Protocols：

Western Blotting (WB) Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol
ELISA Protocol
儲存條件：

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
貨期：

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
用途：

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產品評價

靶點詳情

功能：

Catalyzes the ATP-dependent ligation of histidine to the 3'-end of its cognate tRNA, via the formation of an aminoacyl-adenylate intermediate (His-AMP). Plays a role in axon guidance.
基因功能參考文獻：
1. This study suggests that the human HisRS genes, while descending from a common ancestor with dual function for both types of tRNA(His), have acquired highly specialized tRNA recognition properties through evolution. PMID: 28321488
2. Despite the similar kinetics, differential scanning fluorimetry revealed that Y454S is less thermally stable than Wild Type HARS, and cells from Y454S patients grown at elevated temperatures demonstrate diminished levels of protein synthesis compared to those of Wild Type cells. The thermal sensitivity associated with the Y454S mutation represents a biochemical basis for understanding Usher Syndrome Type IIIB. PMID: 28632987
3. Loss of function mutations in histidyl-tRNA synthetase cause a spectrum of inherited peripheral neuropathies PMID: 26072516
4. Data suggest that by comparing human and trypanosomatid histidyl-tRNA synthetases (HisRS) may provide opportunities for developing specific inhibitors of Trypanosoma brucei HisRS. PMID: 25151410
5. Secreted histidyl-tRNA synthetase splice variants elaborate major epitopes for autoantibodies in inflammatory myositis. PMID: 24898250
6. Data indicate that higher anti-Jo1 levels were associated with disease severity in antisynthetase syndrome (ASS) patients. PMID: 24286268
7. Non-Jo-1 anti-synAb positive patients have decreased survival compared with Jo-1 patients. PMID: 23422076
8. Findings suggest that histidyl-tRNA synthetase (HARS) is associated with axonal peripheral neuropathy. PMID: 22930593
9. The crystal structure of a novel splice variant of a tRNA synthetase lacking the entire catalytic domain. PMID: 22958643
10. Although anti-Jo1 positive patients with antisynthetase syndrome share features of patients with anti-PL7/PL12 antibody, they exhibit many differences regarding clinical phenotype and long-term outcome. PMID: 22326685
11. Study identified sequence variants in the known disease-causing genes SLC6A3 and FLVCR1, and present evidence to strongly support the pathogenicity of variants identified in TUBGCP6, BRAT1, SNIP1, CRADD, and HARS. PMID: 22279524
12. genomic organization of the HARS locus and mapping of transcripts originating from a bi-directional promoter controlling the differential expression of these gene PMID: 12056811
13. Demonstrating histidyl-tRNA synthetase (Jo-1)-specific T cell responses represents a key step in establishing the hypothesis that Jo-1 drives T cell-mediated autoimmunity in Jo-1+ polymyositis. PMID: 12471150
14. the TSG101 interaction with HRS is a crucial step in endocytic down-regulation of mitogenic signaling and this interaction may have a role in linking the functions of early and late endosomes PMID: 12802020
15. A proteolytically sensitive conformation of HisRS exists in the lung, the target tissue associated with this autoantibody response. PMID: 17665459
16. clinical and prognostic profiles of 45 patients displaying anti-histidyl-tRNA synthetase autoantibodies were determined PMID: 17785330
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相關疾病：

Usher syndrome 3B (USH3B); Charcot-Marie-Tooth disease 2W (CMT2W)
亞細胞定位：

Cytoplasm.
蛋白家族：

Class-II aminoacyl-tRNA synthetase family
組織特異性：

Brain, heart, liver and kidney.
數據庫鏈接：

HGNC: 4816

OMIM: 142810

KEGG: hsa:3035

STRING: 9606.ENSP00000425634

UniGene: Hs.528050

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