Recombinant Human DNA repair protein XRCC4 (XRCC4)

中文名稱：

人XRCC4重組蛋白
品名簡稱：

Recombinant Human XRCC4 protein
貨號：

CSB-EP614413HU
說明書：

MSDS Datasheet
規格：

￥1836
圖片：
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
其他：

產品詳情
產品評價
相關產品
靶點詳情

產品詳情

純度：

Greater than 90% as determined by SDS-PAGE.
基因名：

XRCC4
Uniprot No.：

Q13426
別名：

DNA double strand break repair and V(D)J recombination protein XRCC4; DNA repair protein XRCC4; SSMED; X ray repair complementing defective repair in Chinese hamster cells 4; X ray repair cross complementing 4; X ray repair cross complementing protein 4; X-ray repair cross-complementing protein 4; XRCC 4; XRCC4; XRCC4_HUMAN
種屬：

Homo sapiens (Human)
蛋白長度：

Full Length
來源：

E.coli
分子量：

65.3kDa
表達區域：

1-336aa
氨基酸序列

MERKISRIHLVSEPSITHFLQVSWEKTLESGFVITLTDGHSAWTGTVSESEISQEADDMAMEKGKYVGELRKALLSGAGPADVYTFNFSKESCYFFFEKNLKDVSFRLGSFNLEKVENPAEVIRELICYCLDTIAENQAKNEHLQKENERLLRDWNDVQGRFEKCVSAKEALETDLYKRFILVLNEKKTKIRSLHNKLLNAAQEREKDIKQEGETAICSEMTADRDPVYDESTDEESENQTDLSGLASAAVSKDDSIISSLDVTDIAPSRKRRQRMQRNLGTEPKMAPQENQLQEKENSRPDSSLPETSKKEHISAENMSLETLRNSSPEDLFDEI
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.
蛋白標簽：

N-terminal GST-tagged
產品提供形式：

Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
緩沖液：

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
儲存條件：

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
保質期：

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
貨期：

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
注意事項：

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA：

Please contact us to get it.

產品評價

靶點詳情

功能：

DNA non-homologous end joining (NHEJ) core factor, required for double-strand break repair and V(D)J recombination. Acts as a scaffold protein that regulates recruitment of other proteins to DNA double-strand breaks (DSBs). Associates with NHEJ1/XLF to form alternating helical filaments that bridge DNA and act like a bandage, holding together the broken DNA until it is repaired. The XRCC4-NHEJ1/XLF subcomplex binds to the DNA fragments of a DSB in a highly diffusive manner and robustly bridges two independent DNA molecules, holding the broken DNA fragments in close proximity to one other. The mobility of the bridges ensures that the ends remain accessible for further processing by other repair factors. Plays a key role in the NHEJ ligation step of the broken DNA during DSB repair via direct interaction with DNA ligase IV (LIG4): the LIG4-XRCC4 subcomplex reseals the DNA breaks after the gap filling is completed. XRCC4 stabilizes LIG4, regulates its subcellular localization and enhances LIG4's joining activity. Binding of the LIG4-XRCC4 subcomplex to DNA ends is dependent on the assembly of the DNA-dependent protein kinase complex DNA-PK to these DNA ends. Promotes displacement of PNKP from processed strand break termini.; Acts as an activator of the phospholipid scramblase activity of XKR4. This form, which is generated upon caspase-3 (CASP3) cleavage, translocates into the cytoplasm and interacts with XKR4, thereby promoting phosphatidylserine scramblase activity of XKR4 and leading to phosphatidylserine exposure on apoptotic cell surface.
基因功能參考文獻：
1. These data imply that the level of XRCC4 expression could be used to predict the effects of apoptosis-inducing drugs in cancer treatment. PMID: 29233683
2. Three-locus model of gene-gene interactions OGG1 (rs1052133) * ADPRT (rs1136410) * XRCC4 (rs6869366) was associated with high genotoxic risk in coal miners. PMID: 28992182
3. Variant rs3734091 was found to be significantly associated with breast cancer while rs6869366 variant did not show any association. These SNPs may influence the susceptibility of individuals to breast cancer in this Indian population. PMID: 29452234
4. Results showed that eNOS and XRCC4 VNTR variants might play a potential role in schizophrenia + nicotine dependence and/or nicotine dependence pathophysiology. PMID: 29050484
5. Phospho-blocking and -mimicking mutations impact both the stability and DNA bridging capacity of XRCC4/XLF complexes, but without affecting their ability to stimulate LIG4 activity. Implicit in this finding is that phosphorylation may regulate DNA bridging by XRCC4/XLF filaments. PMID: 28500754
6. This study demonstrated both ligase IV and XRCC4 may act in concert to modulate the development of glioma. PMID: 27508978
7. Data suggest that genetic variants of XRCC4 and ERCC1 may independently or jointly affect survival in chemotherapy-treated gastric cancer (GCa) patients by modulating the gene expression in the tumors. PMID: 28796378
8. In a recombinant PNKP-XRCC4-LigIV complex, stable binding of PNKP requires XRCC4 phosphorylation. Only one PNKP protomer binds per XRCC4 dimer. Both the PNKP FHA and catalytic domains contact the XRCC4 coiled-coil and LigIV BRCT repeats. Multipoint contacts between PNKP and XRCC4-LigIV regulate PNKP recruitment and activity within NHEJ. PMID: 28453785
9. Data suggest that stimulation of Artemis nuclease/DCLRE1C activity by XRCC4-DNA ligase IV hetero-complex and efficiency of blunt-end ligation are determined by structural configurations at the DNA ends. (XRCC4 = X-ray repair cross complementing 4) PMID: 28696258
10. the various XRCC4 mutations that lead to primordial dwarfism and their impact on non-homologous end joining and V(D)J recombination are discussed (Review) PMID: 27169690
11. XRCC4 expression might have an influence on results of radiotherapy for patients with esophageal squamous cell carcinoma. PMID: 27338590
12. uterine cervical cancer patients with high Ku86 and XRCC4 expression had a significantly lower 5-year metastasis-free rate than others. PMID: 26867665
13. Frequencies of XRCC4-1394 T/G+G/G genotypes were higher in patients (%34) than the controls (%18.7). The statistical analysis revealed that the individuals who had XRCC4-1394 T/G+G/G genotype had an increased risk for autism spectrum disorder (OR = 2.23, 95% CI = 1.10-4.55) PMID: 27064873
14. Genetic Variations in XRCC4 (rs1805377) is not Associated with Hepatocellular Carcinoma in Thai Patients with Hepatitis B Virus Infection. PMID: 26925648
15. These results suggested potential usefulness of the phosphorylation status of XRCC4 Ser320 as an indicator of DNA-PK functionality in living cells. PMID: 26666690
16. Association between insertion/deletion polymorphism in intron 3 of XRCC4 and susceptibility to type I bipolar disorder PMID: 26484731
17. For XRCC4 (rs1805377) polymorphism, no difference was found in distribution between the ESCC and control groups. PMID: 26166223
18. using dual- and quadruple-trap optical tweezers combined with fluorescence microscopy, we show how human XRCC4, XLF and XRCC4-XLF complexes interact with DNA in real time PMID: 27437582
19. The ends are then closely aligned, which requires XLF, a non-catalytic function of XRCC4-LIG4, and DNA-PK activity PMID: 26990988
20. Polymorphisms of XRCC4 are associated with susceptibility to Colorectal Cancer. PMID: 25662981
21. FBXW7 facilitates nonhomologous end-joining via K63-linked polyubiquitylation of XRCC4 in tumor cells. PMID: 26774286
22. The XRCC4 -1394T/G polymorphism is associated with susceptibility to endometriosis in an Iranian population. PMID: 26088159
23. Our results suggest that the XRCC4 rs2075685 polymorphism could influence the susceptibility to pancreatic cancer in a Chinese population. PMID: 26345895
24. The TMPRSS2-ERG Gene Fusion Blocks XRCC4-Mediated Nonhomologous End-Joining Repair and Radiosensitizes Prostate Cancer Cells to PARP Inhibition PMID: 26026052
25. Point mutations in XRCC4 is associated with microcephaly, short stature and genomic instability. PMID: 25839420
26. Genetic susceptibility to schizophrenia was found in patients with genetic polymorphisms in intron 3 of the XRCC4 gene. PMID: 26112447
27. our results suggest that XRCC4 rs2075685 polymorphism plays an important role in the risk of pancreatic cancer in a Chinese population PMID: 26045837
28. Nonsense mutations in human XRCC4 have recently been associated with primordial dwarfism and, in our cases, with adult-onset neurological impairment, suggesting an important role for DNA repair in the brain. PMID: 25872942
29. Polymorphisms of insertion/deletion at the intron 3 of the XRCC4 is associated with gastric cancer. PMID: 25527410
30. Variants of the DNA damage repair gene XRCC4 increase the risk of esophageal cancer PMID: 25612937
31. no association was found between variants of XRCC4 rs2075685 and XRCC4 rs1805377 and development of glioma PMID: 25973104
32. study identified a novel pathogenic variant in XRCC4; finding expands the spectrum of DNA damage repair syndromes to include XRCC4 deficiency causing severe postnatal growth failure, microcephaly, gonadal failure, metabolic syndrome, and possibly tumor predisposition PMID: 25742519
33. Our data showed that Cd altered the phosphorylation of DNA-PKcs, and reduced the expression of both XRCC4 and Ligase IV in irradiated cells. PMID: 26201248
34. our findings document the role of XRCC4 in non-BRCA1/2 breast cancer PMID: 25360583
35. the intron 3 VNTR polymorphism in the XRCC4 gene may be associated with the etiopathogenesis of RA as a marker of immune aging. PMID: 25494482
36. The present results collectively indicated that Lys271, but not Lys210, of XRCC4 is required for the nuclear localization of XRCC4 and LIG4 and that the nuclear localizing ability is essential for DSB repair function of XRCC4. PMID: 25934149
37. The XRCC4 promoter -652G>T polymorphism is functional and may influence genetic susceptibility to prostate cancer. PMID: 25096509
38. We present comprehensive genetic and cellular evidence that mutations in XRCC4 cause microcephalic primordial dwarfism. PMID: 25728776
39. XRCC4 C-terminal point mutants, R325F and N326L, are functionally deficient in terms of cell survival after irradiation. PMID: 25597996
40. There was longer survival among patients with non-invasive tumours associated with DNA repair X-ray repair cross-complementing protein 4 (XRCC4) heterozygous genotype compared with wild-type. PMID: 24666523
41. Phosphorylation of XRCC4 in mitosis was not responsible for its failure to localize to mitotic chromosomes and phosphorylation did not affect the interaction of XRCC4 with DNA ligase IV PMID: 25165869
42. Taken together, our results indicate that XRCC4 is required not only for the promotion of NHEJ during interphase but also for its M-phase-specific suppression of DSB repair. PMID: 25166505
43. XRCC4 codon 247 polymorphism may be associated with DIA risk and prognosis among the Guangxi population. PMID: 24378850
44. Results show that in cells deficient for Lig4, chromosomal translocations junctions had significantly longer deletions and more microhomologies. PMID: 25201414
45. Characterization of the protein interaction domains that modulate the XRCC4/Ligase IV interaction. PMID: 23794378
46. Single nucleotide polymorphisms in XRCC4 were associated with breast cancer risk. PMID: 24062231
47. These results indicate for the first time that DNA ligase IV (LIG4) rs1805388 and X-ray Repair Cross Complementing-4 (XRCC4) rs1805377, alone or in combination, are associated with a risk of gliomas. PMID: 23663450
48. The induced DNA damage by Methyl Methanesulfonate increases in Chronic Obstructive Pulmonary Disease patients with variant genotypes in OGG1 (Ser326Cys) showing impairment of DNA repair. PMID: 24053728
49. XRCC4P may be a genetic modifier for the risk and outcome of hepatocellular carcinoma induced by aflatoxin B1 exposure. PMID: 23788213
50. The chromatin binding of XRCC4 was dependent on the presence of LIG4. PMID: 23994631
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相關疾病：

Short stature, microcephaly, and endocrine dysfunction (SSMED)
亞細胞定位：

Nucleus. Chromosome.; [Protein XRCC4, C-terminus]: Cytoplasm.
蛋白家族：

XRCC4 family
組織特異性：

Widely expressed.
數據庫鏈接：

HGNC: 12831

OMIM: 194363

KEGG: hsa:7518

STRING: 9606.ENSP00000342011

UniGene: Hs.567359