Recombinant Human DNA mismatch repair protein Mlh3 (MLH3), partial

中文名稱：

人MLH3重組蛋白
貨號：

CSB-YP883389HU
說明書：

MSDS Datasheet
規格：
來源：

Yeast
其他：

中文名稱：

人MLH3重組蛋白
貨號：

CSB-EP883389HU
說明書：

MSDS Datasheet
規格：
來源：

E.coli
其他：

中文名稱：

人MLH3重組蛋白
貨號：

CSB-EP883389HU-B
說明書：

MSDS Datasheet
規格：
來源：

E.coli
共軛：

Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
其他：

中文名稱：

人MLH3重組蛋白
貨號：

CSB-BP883389HU
說明書：

MSDS Datasheet
規格：
來源：

Baculovirus
其他：

中文名稱：

人MLH3重組蛋白
貨號：

CSB-MP883389HU
說明書：

MSDS Datasheet
規格：
來源：

Mammalian cell
其他：

產品詳情
產品評價
相關產品
靶點詳情

產品詳情

純度：

>85% (SDS-PAGE)
基因名：

MLH3
Uniprot No.：

Q9UHC1
別名：

DNA mismatch repair protein Mlh3; HNPCC 7; HNPCC; HNPCC7; MGC138372; Mismatch repair gene MLH 3; Mismatch repair gene MLH3; MLH 3; MLH3; MLH3_HUMAN; MutL homolog 3 (E. coli); MutL homolog 3; MutL protein homolog 3; S240II117
種屬：

Homo sapiens (Human)
蛋白長度：

Partial
蛋白標簽：

Tag?type?will?be?determined?during?the?manufacturing?process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
產品提供形式：

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
復溶：

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
儲存條件：

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
保質期：

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
貨期：

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
注意事項：

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet ：

Please contact us to get it.

產品評價

靶點詳情

功能：

Probably involved in the repair of mismatches in DNA.
基因功能參考文獻：
1. The effects of chronic smoking on oral mucosa led to the methylation of genes MRE11A PMS2, XRCC1 and MLH3, but resulted in a reduction of gene expression of MRE11A and PMS2, which showed >/=50% methylation. These results provide evidence that smoking cause methylation and reduced expression of repair genes. PMID: 29775861
2. MLH3 germline variants are associated with colon cancer patients belonging to families with Lynch syndrome-associated brain tumors. PMID: 27401157
3. A polymorphism within the MLH3 gene is associated with oligozoospermia in Caucasian men of a certain area. PMID: 26520453
4. MutL homolog 3 (MLH3) promoter methylation was observed in 61% of oligoastrocytoma and 27% of astrocytoma. PMID: 26303387
5. Results indicate that CT(844)-CC(942) was associated with a high risk of cervical carcinoma and cervical intraepithelial neoplasia, and the CC(844)-CT(942) decreased the risk. PMID: 24759751
6. The experiments show recruitment and persistence of MutLgamma-heterodimers at UVA-induced DNA lesions. PMID: 23696135
7. the MutSbeta-MutLalpha interaction is mediated in part by residues ((L/I)SRFF) embedded within the MSH3 PCNA-binding motif PMID: 20154325
8. hMLH3 mRNA is present at low levels in numerous tissues but high levels in testis. hMLH3 functions in meiosis as well as hMSH2-hMSH3 repair processes & has little if any role in Hereditary Non-Polyposis Colorectal Cancer (HNPCC). PMID: 19483466
9. There is an association of polymorphism C85T in MSH5 or C2531T in MLH3 with male infertility, specifically azoospermia or severe oligozoospermia, and interaction between these MSH5 and MLH3 polymorphisms increased the risk of developing male infertility PMID: 19808033
10. Little evidence for involvement of MLH3 in colorectal cancer predisposition. PMID: 12800209
11. at pachynema, when chromosomes are fully paired, we find significant heterogeneity in the localization of the MutL homologs, MLH1 and MLH3, among human oocyte populations PMID: 15558497
12. The identification of inherited missense variants, somatic missense mutations (present in 3 of 57 tumors), and LOH in the tumor from a patient with a germ line missense change suggest a role for MLH3 in endometrial tumorigenesis. PMID: 16885347
13. Mutations of Mlh3 may work together with other genes in an accumulated manner and result in an increased risk of esophageal tumor PMID: 16981255
14. in absence of hPMS2, hMLH3 (hMutLgamma) is located in the nucleus, suggesting a conditional activity in MMR and supporting its role as a low-risk gene in hereditary non-polyposis colorectal cancer PMID: 17203173
15. Two simultaneous hMLH3 variants might predispose to spermatogenic arrest. PMID: 17482610
16. results suggest that the endonuclease activity of MutLalpha is important not only in MMR-dependent mutation avoidance but also for recombination and damage response functions PMID: 17567544
17. MLH3 and EXO1 alterations in familial colorectal cancer patients not fulfilling Amsterdam criteria. PMID: 17656264
18. To assess the significance of the inherited sequence variations in MLH3, we functionally characterized seven missense mutations PMID: 18521850
19. Mlh3 nullizygosity significantly increased Apc frameshift mutations and tumor multiplicity. PMID: 18551179
20. the different biochemical assays yielded no evidence that the eight MLH3 unclassified variants (missense mutations) tested are the cause of hereditary colorectal cancer, including Lynch syndrome PMID: 19156873
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相關疾病：

Hereditary non-polyposis colorectal cancer 7 (HNPCC7); Colorectal cancer (CRC)
亞細胞定位：

Nucleus.
蛋白家族：

DNA mismatch repair MutL/HexB family
組織特異性：

Ubiquitous.
數據庫鏈接：

HGNC: 7128

OMIM: 114500

KEGG: hsa:27030

STRING: 9606.ENSP00000348020

UniGene: Hs.436650