E. coli biotin ligase
(BirA) is highly specific in covalently attaching biotin to the 15
amino
acid AviTag peptide. This recombinant protein was biotinylated in
vivo
by AviTag-BirA technology, which method is BriA catalyzes amide
linkage
between the biotin and the specific lysine of the AviTag.
The tag type will
be
determined during production process. If you have specified tag
type, please tell us and we will develop the specified tag
preferentially.
產(chǎn)品提供形式:
Lyophilized powder
Note: We will
preferentially ship the format that we have in stock, however,
if you have any special requirement for the format, please
remark your requirement when placing the order, we will prepare
according to your demand.
復溶:
We recommend that this vial be briefly centrifuged
prior
to opening to bring the contents to the bottom. Please reconstitute
protein in deionized sterile water to a concentration of 0.1-1.0
mg/mL.We recommend to add 5-50% of glycerol (final concentration)
and
aliquot for long-term storage at -20℃/-80℃. Our default final
concentration of glycerol is 50%. Customers could use it as
reference.
儲存條件:
Store at -20°C/-80°C upon receipt, aliquoting is
necessary for
mutiple use. Avoid repeated freeze-thaw cycles.
保質(zhì)期:
The shelf life is related to many factors, storage
state,
buffer ingredients, storage temperature and the stability of the
protein
itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C.
The
shelf life of lyophilized form is 12 months at -20°C/-80°C.
貨期:
Delivery time may
differ from different purchasing way or location, please kindly
consult your local distributors for specific delivery time.
Note: All of our
proteins are default shipped with normal blue ice packs, if you
request to ship with dry ice, please communicate with us in
advance
and extra fees will be charged.
注意事項:
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Although its physiological function is unclear, it can inhibit neutrophil cathepsin G and mast cell chymase, both of which can convert angiotensin-1 to the active angiotensin-2.
基因功能參考文獻:
SERPINA3 silencing inhibits the migration, invasion, and liver metastasis of colon cancer cells. PMID: 29855767
evaluation of serum proteins reveals that SERPINA1, SERPINA3 and SERPINC1 could be useful to discriminate healthy from colorectal carcinoma patients with a high sensitivity and specificity PMID: 27492143
This study confirms the prognostic potential of SERPINA3 expression in human cutaneous melanoma PMID: 27213583
AACT is downregulated in NSCLC. The GlcNAcylated AACT can effectively differentiate Stage I non-small cell lung cancer from healthy or benign lung disease samples. PMID: 26908325
The ACT gene (also named SERPINA3) was mapped on chromosome 14q32.1 We studied the association between Alpha-1 antichymotrypsin (ACT) signal peptide A/T polymorphism polymorphism and risk of primary intracerebral hemorrhage PMID: 26210716
Proteome demonstration of alpha-1-acid glycoprotein and alpha-1-antichymotrypsin candidate biomarkers for diagnosis of enterovirus 71 infection PMID: 25170552
SERPINA3 promotes endometrial cancer cells growth by regulating G2/M cell cycle checkpoint and apoptosis. PMID: 24817931
ACT-17 variation presents a risk factor for Alzheimer's disease in the worldwide population, especially in the Caucasian population. PMID: 22272609
This study demonistrated that SERPINA3 is changed in the dorsolateral prefrontal cortex of individuals with schizophrenia PMID: 22869038
Prostate specific antigen (PSA) forms complexes with alpha1-antichymotrypsin (ACT) within the prostate, which may explain, at least partially, why the ratio of serum PSA-ACT to total PSA is increased in prostate cancer. PMID: 22806587
the promoter of SERPINA3 is transcriptionally activated by three transcription factors (SP1, MZF1 and ZBTB7B) PMID: 22246292
The common alpha1-antichymotrypsin signal peptide -15A/T polymorphism may not be a major risk factor for Alzheimer disease (AD) but the polymorphism is capable of increasing late onset AD risk. [Meta-analysis] PMID: 22294107
Pivotal role for alpha1-antichymotrypsin in skin repair PMID: 21693707
alpha-1 antichymotrypsin C-terminal fragment and Apo A1 provide a plasma molecular profile, and this is associated with acute cellular renal allograft rejection. PMID: 21730889
human plasma serine protease inhibitor alpha(1)-antichymotrypsin inhibits staphopain C PMID: 21296644
The Alpha 1 antichymotrypsin is an independent histological prognostic marker for patients with Melanoma, especially those with Stage III melanoma. PMID: 20444200
Found a marked and persistent coronary artery bypass grafting (CABG) postoperative increase in the levels of the serpin-protease inhibitor alpha(1)-antichymotrypsin that fully maintains the inhibitory activity blunting its protease substrate cathepsin G. PMID: 20302328
The study concluded that the effect of the variations in the alpha-1-antichymotrypsin regulatory region must be very limited. PMID: 20378355
The ANTICHYMOTRYPSIN genotype and allele frequency are not different in Indian cerebral hemorrhage and cerebral ischemia compared to controls. PMID: 19959196
No cirrhosis secondary to ACT deficiency has been reported in children. PMID: 20158662
AAT efficiently inhibits both heat- and chemical-induced aggregation of various test proteins. PMID: 20117085
The rs4934 polymorphism of SERPINA3 is not associated with sporadic intracranial aneurysms among individuals of Chinese Han ethnicity. PMID: 19907165
alpha(1)-antichymotrypsin polymorphism: a risk factor for hemorrhagic stroke in normotensive subjects. PMID: 11692021
A25G and G39A substitutions in the AACT gene are probably one of the risk factors to Alzheimer's disease (AD) in Han Chinese. PMID: 11798857
no association seen between alpha 1-antichymotrypsin and time to psychosis in Alzheimer's disease PMID: 11936240
significantly higher in plasma of Alzheimer patients compared to controls PMID: 11959399
data suggested that the ApoE epsilon2/epsilon4 genotype might be a susceptibility variant of moderate effect for sporadic idiopathic Parkinson disease in our samples, whereas the ACT gene signal peptide polymorphism might not PMID: 11992569
Gene regulation of the serine proteinase inhibitors alpha1-antitrypsin and alpha1-antichymotrypsin. PMID: 12023832
alpha(1)-antitrypsin and antichymotrypsin are produced by the mammary gland and are present in milk in relatively high amounts in early lactation PMID: 12324297
Polymeric AACT specifically seeds the polymerization of native protein by a serpin-dependent process that occurs under physiological conditions and may be important in the deposition of AACT in plaques in patients with Alzheimer's disease. PMID: 12600202
overexpression of ACT by astrocytes could thus contribute to increased beta-amyloid fibril formation in Alzheimer's disease PMID: 15530656
Alpha1-antichymotrypsin-AA may confer a modest protection against developing early-onset Parkinson disease in women PMID: 15542006
Multivariate analysis showed that ACT mRNA level, but not STC2 mRNA level, in HR-positive patients, was a significant prognostic factor (P = 0.042), which was independent of tumor size and lymph node metastases. PMID: 15546506
The ACT TT promoter genotype was associated with an increased risk of EOAD independently from the presence of the apolipoprotein E (APOE) epsilon 4 allele. PMID: 15653173
serpin/furin complex stability depends on pH and regulation at the deacylation step PMID: 15659365
the A/T polymorphism of SERPINA3 gene is associated with the risk factor for aneurysmal subarachnoid hemorrhage PMID: 15718509
The onset of MSA was significantly earlier and the disease progressed significantly faster in patients with ACT-AA genotype than in those with non-ACT-AA genotypes. The ACT concentration in cerebrospinal fluid was increased in patients with ACT-AA. PMID: 15907346
This study findings suggest that ACT polymorphism (A/T) is a risk factor for post-stroke dementia. PMID: 15935385
AP-1 is a key transcription factor that, in part, controls astrocyte-specific expression of genes including the ACT and GFAP genes PMID: 16303762
SERPINA3 polymorphism does not have a role in primary intracerebral hemorrhage in a Polish population PMID: 16424370
NFI-X cooperates with (activator protein 1)AP-1 by an unknown mechanism in astrocytes, which results in the expression of a subset of astrocyte-specific genes. PMID: 16565071
acute myocardial infarction from information on functional gene variants that favor inflammation or modulate cholesterol metabolism: IL6 -174 G/C, TNF -308 G/A, IL10 -1082 G/A, SERPINA3 -51 G/T, IFNG +874 T/A, HMGCR -911 C/A, and APOE epsilon2/3/4 PMID: 18056971
We conclude that APOE is a risk gene for amnestic mild cognitive impairment and that ACT and CHRNA7 may act in these patients as modifier genes for the time of progression to Alzheimer's disease. PMID: 18078695
Nur77 overexpression and RNA interference-mediated Nur77 gene knockdown analysis confirmed that SerpinA3 is indeed a novel Nur77-targeted gene. PMID: 18248459
A correlation of PSA levels and the alpha 1 antichymotrypsin genotype in young prostate carcinoma patients was found. PMID: 18383875
Alpha-1-antichymotrypsin is a pathophysiologic inhibitor that controls MMP-9 activation in skin tissue. PMID: 18401426
Our study did not show an association between alpha-1 antichymotrypsin signal peptide A/T polymorphism and primary intracerebral hemorrhage. PMID: 18408372
increased peripheral ACT levels in APOE 4 positive patients appear to predict an accelerated clinical progression PMID: 18991685
Ox-ACT promotes release of human monocyte chemoattractant protein-1 (MCP-1) and IL-8 from human lung type epithelial cells (A549) and normal human bronchial epithelial (NHBE) cells PMID: 19525388
Using shotgun mass spectrometry, we found this protein differentially expressed in the dorsolateral prefrontal cortex from patients with schizophrenia. PMID: 19165527
顯示更多
收起更多
亞細胞定位:
Secreted.
蛋白家族:
Serpin family
組織特異性:
Plasma. Synthesized in the liver. Like the related alpha-1-antitrypsin, its concentration increases in the acute phase of inflammation or infection. Found in the amyloid plaques from the hippocampus of Alzheimer disease brains.