Recombinant Escherichia coli tRNA threonylcarbamoyladenosine biosynthesis protein TsaE (tsaE)

中文名稱：

大腸桿菌tsaE重組蛋白
貨號：

CSB-YP360227ENV
說明書：

MSDS Datasheet
規(guī)格：
來源：

Yeast
其他：

中文名稱：

大腸桿菌tsaE重組蛋白
貨號：

CSB-EP360227ENV-B
說明書：

MSDS Datasheet
規(guī)格：
來源：

E.coli
共軛：

Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
其他：

中文名稱：

大腸桿菌tsaE重組蛋白
貨號：

CSB-BP360227ENV
說明書：

MSDS Datasheet
規(guī)格：
來源：

Baculovirus
其他：

產(chǎn)品詳情
產(chǎn)品評價
相關(guān)產(chǎn)品
靶點詳情

產(chǎn)品詳情

純度：

>85% (SDS-PAGE)
基因名：

tsaE
Uniprot No.：

P0AF67
別名：

tsaE; yjeE; b4168; JW4126tRNA threonylcarbamoyladenosine biosynthesis protein TsaE; t(6)A37 threonylcarbamoyladenosine biosynthesis protein TsaE
種屬：

Escherichia coli (strain K12)
蛋白長度：

full length protein
表達區(qū)域：

1-153
氨基酸序列

MMNRVIPLPD EQATLDLGER VAKACDGATV IYLYGDLGAG KTTFSRGFLQ ALGHQGNVKS PTYTLVEPYT LDNLMVYHFD LYRLADPEEL EFMGIRDYFA NDAICLVEWP QQGTGVLPDP DVEIHIDYQA QGREARVSAV SSAGELLLAR LAG
蛋白標簽：

Tag?type?will?be?determined?during?the?manufacturing?process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
產(chǎn)品提供形式：

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
復(fù)溶：

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
儲存條件：

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
保質(zhì)期：

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
貨期：

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
注意事項：

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet ：

Please contact us to get it.

產(chǎn)品評價

靶點詳情

功能：

Required for the formation of a threonylcarbamoyl group on adenosine at position 37 (t(6)A37) in tRNAs that read codons beginning with adenine. Is probably involved in the transfer of the threonylcarbamoyl moiety of threonylcarbamoyl-AMP (TC-AMP) to the N6 group of A37, together with TsaD and TsaB. TsaE seems to play an indirect role in the t(6)A biosynthesis pathway, possibly in regulating the core enzymatic function of TsaD. Displays ATPase activity in vitro.
基因功能參考文獻：
1. high-affinity binding of YjeE to ADP using equilibrium dialysis; YjeE hydrolyses ATP PMID: 15324301
2. Repression of yjeE expression results in at least a proportion of very enlarged cells with an unusual peripheral distribution of DNA, showing that this gene is essential for the viability of E. coli. PMID: 19376873
亞細胞定位：

Cytoplasm.
蛋白家族：

TsaE family
數(shù)據(jù)庫鏈接：

KEGG: ecj:JW4126

STRING: 316385.ECDH10B_4363