Recombinant Escherichia coli tRNA threonylcarbamoyladenosine biosynthesis protein TsaE (tsaE)

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中文名稱(chēng)：

大腸桿菌tsaE重組蛋白
品名簡(jiǎn)稱(chēng)：

Recombinant E.coli tsaE protein
貨號(hào)：

CSB-EP360227ENVe1
說(shuō)明書(shū)：

MSDS Datasheet
規(guī)格：

￥3324
圖片：
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
其他：

產(chǎn)品詳情
產(chǎn)品評(píng)價(jià)
相關(guān)產(chǎn)品
靶點(diǎn)詳情

產(chǎn)品詳情

純度：

Greater than 90% as determined by SDS-PAGE.
基因名：

tsaE
Uniprot No.：

P0AF67
別名：

tsaE; yjeE; b4168; JW4126tRNA threonylcarbamoyladenosine biosynthesis protein TsaE; t(6)A37 threonylcarbamoyladenosine biosynthesis protein TsaE
種屬：

Escherichia coli (strain K12)
蛋白長(zhǎng)度：

Full Length
來(lái)源：

E.coli
分子量：

16.9kDa
表達(dá)區(qū)域：

1-153aa
氨基酸序列

MMNRVIPLPDEQATLDLGERVAKACDGATVIYLYGDLGAGKTTFSRGFLQALGHQGNVKSPTYTLVEPYTLDNLMVYHFDLYRLADPEELEFMGIRDYFANDAICLVEWPQQGTGVLPDPDVEIHIDYQAQGREARVSAVSSAGELLLARLAG
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.
蛋白標(biāo)簽：

Tag-Free
產(chǎn)品提供形式：

Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
緩沖液：

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
儲(chǔ)存條件：

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
保質(zhì)期：

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
貨期：

3-7 business days
注意事項(xiàng)：

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA：

Please contact us to get it.

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

功能：

Required for the formation of a threonylcarbamoyl group on adenosine at position 37 (t(6)A37) in tRNAs that read codons beginning with adenine. Is probably involved in the transfer of the threonylcarbamoyl moiety of threonylcarbamoyl-AMP (TC-AMP) to the N6 group of A37, together with TsaD and TsaB. TsaE seems to play an indirect role in the t(6)A biosynthesis pathway, possibly in regulating the core enzymatic function of TsaD. Displays ATPase activity in vitro.
基因功能參考文獻(xiàn)：
1. high-affinity binding of YjeE to ADP using equilibrium dialysis; YjeE hydrolyses ATP PMID: 15324301
2. Repression of yjeE expression results in at least a proportion of very enlarged cells with an unusual peripheral distribution of DNA, showing that this gene is essential for the viability of E. coli. PMID: 19376873
亞細(xì)胞定位：

Cytoplasm.
蛋白家族：

TsaE family
數(shù)據(jù)庫(kù)鏈接：

KEGG: ecj:JW4126

STRING: 316385.ECDH10B_4363