Recombinant Escherichia coli Methionine aminopeptidase (map)

中文名稱：

大腸桿菌map重組蛋白
貨號(hào)：

CSB-YP360042ENV
說明書：

MSDS Datasheet
規(guī)格：
來源：

Yeast
其他：

中文名稱：

大腸桿菌map重組蛋白
貨號(hào)：

CSB-EP360042ENV-B
說明書：

MSDS Datasheet
規(guī)格：
來源：

E.coli
共軛：

Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
其他：

中文名稱：

大腸桿菌map重組蛋白
貨號(hào)：

CSB-BP360042ENV
說明書：

MSDS Datasheet
規(guī)格：
來源：

Baculovirus
其他：

中文名稱：

大腸桿菌map重組蛋白
貨號(hào)：

CSB-MP360042ENV
說明書：

MSDS Datasheet
規(guī)格：
來源：

Mammalian cell
其他：

產(chǎn)品詳情
產(chǎn)品評(píng)價(jià)
相關(guān)產(chǎn)品
靶點(diǎn)詳情

產(chǎn)品詳情

純度：

>85% (SDS-PAGE)
基因名：

map
Uniprot No.：

P0AE18
別名：

map; b0168; JW0163Methionine aminopeptidase; MAP; MetAP; EC 3.4.11.18; Peptidase M
種屬：

Escherichia coli (strain K12)
蛋白長度：

Full Length of Mature Protein
表達(dá)區(qū)域：

2-264
氨基酸序列

AISIKTPED IEKMRVAGRL AAEVLEMIEP YVKPGVSTGE LDRICNDYIV NEQHAVSACL GYHGYPKSVC ISINEVVCHG IPDDAKLLKD GDIVNIDVTV IKDGFHGDTS KMFIVGKPTI MGERLCRITQ ESLYLALRMV KPGINLREIG AAIQKFVEAE GFSVVREYCG HGIGRGFHEE PQVLHYDSRE TNVVLKPGMT FTIEPMVNAG KKEIRTMKDG WTVKTKDRSL SAQYEHTIVV TDNGCEILTL RKDDTIPAII SHDE
蛋白標(biāo)簽：

Tag?type?will?be?determined?during?the?manufacturing?process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
產(chǎn)品提供形式：

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
復(fù)溶：

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
儲(chǔ)存條件：

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
保質(zhì)期：

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
貨期：

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
注意事項(xiàng)：

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet ：

Please contact us to get it.

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

功能：

Removes the N-terminal methionine from nascent proteins. The N-terminal methionine is often cleaved when the second residue in the primary sequence is small and uncharged (Met-Ala-, Cys, Gly, Pro, Ser, Thr, or Val). Requires deformylation of the N(alpha)-formylated initiator methionine before it can be hydrolyzed.
基因功能參考文獻(xiàn)：
1. These data suggest that map expression contributes to the maintenance of enteropathogenic Escherichia coli colonization. PMID: 25312947
2. Methionine aminopeptidase associates with ribosomes via a charged loop crucial for nascent-chain processing & cell viability. It competes with peptide deformylase for binding sites at the ribosomal tunnel exit PMID: 23770820
3. Data show that Europium ion (Eu(3+)) binds to methionine aminopeptidase and binding results in the activation of the enzyme. PMID: 22112844
4. obtained a crystal structure for a complex of Escherichia coli methionine aminopeptidase with norleucine phosphonate, a transition-state analog, and only a single Mn(II) ion bound at the active site PMID: 16769889
蛋白家族：

Peptidase M24A family, Methionine aminopeptidase type 1 subfamily
數(shù)據(jù)庫鏈接：

KEGG: ecj:JW0163

STRING: 316385.ECDH10B_0147