map Antibody

^{Rare Species}

說明書

產品詳情

產品名稱：

Rabbit anti-Escherichia coli (strain K12) map Polyclonal antibody
Uniprot No.：

P0AE18
基因名：

map
別名：

Methionine aminopeptidase (MAP) (MetAP) (EC 3.4.11.18) (Peptidase M) map b0168 JW0163
宿主：

Rabbit
反應種屬：

Escherichia coli (strain K12)
免疫原：

Recombinant Escherichia coli (strain K12) map protein (2-264aa)
免疫原種屬：

Escherichia coli (strain K12)
標記方式：

Non-conjugated
克隆類型：

Polyclonal
抗體亞型：

IgG
純化方式：

>95%, Protein G purified
濃度：

It differs from different batches. Please contact us to confirm it.
保存緩沖液：

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
產品提供形式：

Liquid
應用范圍：

ELISA, WB

推薦稀釋比：

Application	Recommended Dilution
WB	1:500-1:5000

Protocols：

ELISA Protocol
Western Blotting (WB) Protocol
儲存條件：

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
貨期：

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
用途：

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

功能：

Removes the N-terminal methionine from nascent proteins. The N-terminal methionine is often cleaved when the second residue in the primary sequence is small and uncharged (Met-Ala-, Cys, Gly, Pro, Ser, Thr, or Val). Requires deformylation of the N(alpha)-formylated initiator methionine before it can be hydrolyzed.
基因功能參考文獻：
1. These data suggest that map expression contributes to the maintenance of enteropathogenic Escherichia coli colonization. PMID: 25312947
2. Methionine aminopeptidase associates with ribosomes via a charged loop crucial for nascent-chain processing & cell viability. It competes with peptide deformylase for binding sites at the ribosomal tunnel exit PMID: 23770820
3. Data show that Europium ion (Eu(3+)) binds to methionine aminopeptidase and binding results in the activation of the enzyme. PMID: 22112844
4. obtained a crystal structure for a complex of Escherichia coli methionine aminopeptidase with norleucine phosphonate, a transition-state analog, and only a single Mn(II) ion bound at the active site PMID: 16769889
蛋白家族：

Peptidase M24A family, Methionine aminopeptidase type 1 subfamily
數據庫鏈接：

KEGG: ecj:JW0163

STRING: 316385.ECDH10B_0147

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