Phospho-STAT5A (Y694) Recombinant Monoclonal Antibody

說明書

中文名稱：

磷酸化-STAT5A (Y694)重組抗體
貨號：

CSB-RA022814A694phHU
規(guī)格：

￥1320
圖片：
Western Blot
Positive WB detected in HepG2 whole cell lysate(treated with EGF or not)
All lanes Phospho-STAT5A antibody at 1.025μg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 90 KDa
Observed band size: 90 KDa

IHC image of CSB-RA022814A694phHU diluted at 1:100 and staining in paraffin-embedded human breast cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4℃ overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
其他：

產(chǎn)品詳情
產(chǎn)品評價
相關(guān)產(chǎn)品
靶點詳情

產(chǎn)品詳情

產(chǎn)品描述：

CSB-RA022814A694phHU Phospho-STAT5A (Y694) Recombinant Monoclonal Antibody是一款針對STAT5A蛋白Y694位點磷酸化修飾的高特異性抗體。STAT5A作為JAK-STAT信號通路的核心轉(zhuǎn)錄因子，其酪氨酸磷酸化是激活下游基因調(diào)控功能的關(guān)鍵步驟，在細胞增殖、分化及免疫應(yīng)答中發(fā)揮重要作用。本抗體通過重組單克隆技術(shù)制備，經(jīng)ELISA、Western Blot（WB）和免疫組化（IHC）多平臺驗證，可精準識別磷酸化STAT5A（Y694），且不與其他STAT家族成員或非磷酸化形式發(fā)生交叉反應(yīng)。在WB實驗中推薦使用1:500-1:5000稀釋度，IHC建議1:50-1:200稀釋范圍，適用于檢測多種哺乳動物樣本中STAT5A的激活狀態(tài)。該產(chǎn)品可廣泛應(yīng)用于腫瘤生物學(xué)研究（如白血病、乳腺癌等STAT5異常激活疾病的機制探索）、免疫細胞信號轉(zhuǎn)導(dǎo)分析以及生長發(fā)育相關(guān)的基礎(chǔ)研究領(lǐng)域，為解析磷酸化依賴的細胞功能調(diào)控提供可靠工具，嚴格限定于科學(xué)研究用途。
Uniprot No.：

P42229
基因名：

STAT5A
別名：

MGF antibody; Signal transducer and activator of transcription 5A antibody; Signal Transducer and Activator of Transcription 5B antibody; STA5A_HUMAN antibody; STAT 5A antibody; STAT 5B antibody; STAT5 antibody; STAT5A antibody; STAT5B antibody; Transcription factor STAT5A antibody; Transcription factor STAT5B antibody
反應(yīng)種屬：

Human
免疫原：

A synthesized peptide derived from Human Phospho-STAT5A (Y694)
免疫原種屬：

Homo sapiens (Human)
標記方式：

Non-conjugated
克隆類型：

Monoclonal
抗體亞型：

Rabbit IgG
純化方式：

Affinity-chromatography
克隆號：

3F1
濃度：

It differs from different batches. Please contact us to confirm it.
保存緩沖液：

Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.
產(chǎn)品提供形式：

Liquid
應(yīng)用范圍：

ELISA, WB, IHC

推薦稀釋比：

Application	Recommended Dilution
WB	1:500-1:5000
IHC	1:50-1:200

Protocols：

ELISA Protocol
Western Blotting (WB) Protocol
Immunohistochemistry (IHC) Protocol
儲存條件：

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
貨期：

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
用途：

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產(chǎn)品評價

靶點詳情

功能：

Carries out a dual signal transduction and activation of transcription. Mediates cellular responses to the cytokine KITLG/SCF and other growth factors. Mediates cellular responses to ERBB4. May mediate cellular responses to activated FGFR1, FGFR2, FGFR3 and FGFR4. Binds to the GAS element and activates PRL-induced transcription. Regulates the expression of milk proteins during lactation.
基因功能參考文獻：
1. HSP27 is a partner of JAK2-STAT5 and a potential therapeutic target in myelofibrosis. PMID: 29650953
2. the two cell lines exhibited relatively low protein expression levels of p53, lower levels of p53 and TPp53BP1 transcripts were detected in the K562/G cells. Taken together, these findings suggest that the resistance of CML to the tyrosine kinase inhibitor, imatinib, may be associated with persistent STAT5-mediated ROS production, and the abnormality of the p53 pathway PMID: 29115375
3. Peripheral blood Tregs failed to effectively utilize IL-2 and had relatively little STAT5 phosphorylation in active ankylosing spondylitis. PMID: 27901054
4. These results indicate that IL-3 regulates endothelial cells-extracellular vesicles release, cargo and IL-3 angiogenic paracrine action via STAT5. PMID: 27157262
5. Similar to normal developmental programs, oncogenic functions of STAT5 rely on molecular crosstalk with PI3K/AKT signaling for the initiation, and in some instances the progression, of breast cancer. (Review) PMID: 28495456
6. we demonstrated that Imatinib mesylate (IM)impaired T cell survival through the inhibition of IL-7 and STAT5-p but not TCR signaling which remained unaffected during IM therapy. Thus, off-target inhibitory effects of IM on IL-7 and STAT5-p explain how T cell lymphopenia occurs in patients treated with IM. PMID: 28387753
7. Our results suggest the regulation of STAT5A via epigenetic mechanisms during normal pregnancy and the association of STAT5A epigenetic dysregulation in pregnancy related complications PMID: 27452437
8. Two p53 binding sites were mapped in the STAT5A gene and named PBS1 and PBS2; these sites were sufficient to confer p53 responsiveness in a luciferase reporter gene. PMID: 26876578
9. STAT3/miR-211/STAT5A signaling plays a key role in mesenchymal stem cell migration. PMID: 27145179
10. Stat5 activation increased the DNA binding activity of NF-kappaB though binding of p-Stat5 and p-RelA in nucleus. PMID: 27027438
11. This is the first report of a survival disadvantage of EBV+ patients with CLL, and the first time that STAT5b expression is correlated with survival. The correlation of STAT5 expression with the presence of the virus, along with our survival correlations defines a subgroup of patients with CLL that may benefit from anti-STAT agents. PMID: 27367207
12. STAT5 signaling axis drives abnormal cell proliferation in autosomal dominant polycystic kidney disease. PMID: 28104302
13. O-GlcNAcylation and tyrosine phosphorylation act together to trigger pYSTAT5 levels and oncogenic transcription in neoplastic cells. A mutated hyperactive gain-of-function (GOF) STAT5 without O-GlcNAcylation resulted in decreased tyrosine phosphorylation, oligomerization and transactivation potential and complete loss of oncogenic transformation capacity. PMID: 28074064
14. STAT5 interacted with minichromosome maintenance (MCM) complex, suggesting that STAT5 directly facilitates viral DNA replication by recruiting the helicase complex of the cellular DNA replication machinery to viral DNA replication centers. PMID: 28459842
15. High STAT5 phosphorylation is associated with systemic lupus erythematosus. PMID: 28254841
16. NPM1 downregulation by P-STAT5 is mediated by impairing the BRCA1-BARD1 ubiquitin ligase, which controls the stability of NPM1. In turn, decreased NPM1 levels led to suppression of p53 expression, resulting in enhanced cell survival. PMID: 28005077
17. High STAT5A expression is associated with B-lymphoblastic lymphoma with inflammation. PMID: 27018255
18. Results provide evidence that HDAC6 could regulate HMGN2 acetylation levels and binding to Stat5a-responsive promoters, and therefore, Stat5a transcriptional activity in breast cancer cells. PMID: 27358110
19. Sustained STAT5 transcription factor (STAT5) phosphorylation is necessary to induce long-term interleukin 2 receptor subunit alpha (CD25) expression in T lymphocytes. PMID: 27936140
20. BCR/ABL positively regulates the expression of EZH2 via STAT5 signaling. PMID: 27070757
21. these results demonstrate that the oncogenic signal transducer and activator of transcription 5/Akt pathway is a cellular target for indole-3-carbinol in chronic myeloid leukemia cells. Thus, this clinically tested natural compound can be a potential candidate in the treatment of chronic myeloid leukemia following confirmation with clinical studies PMID: 28631564
22. elucidate a novel mechanism whereby the linker histone H1 prevents STAT5 binding at promoter DNA, and the PRL-induced dissociation of H1 mediated by HMGN2 is necessary to allow full STAT5 recruitment and promote the biological effects of PRL signaling PMID: 28035005
23. These results reveal a novel role of IL-7 and IL-15 in maintaining human T cell function, provide an explanation for T cell dysfunction in humanized mice, and have significant implications for in vitro studies with human T cells. PMID: 27855183
24. STAT5 activation in the dermal papilla is important for hair follicle growth inductaion. PMID: 27131881
25. Data show that signal transducer and activator of transcription 5 STAT5A and STAT5B have distinctive and potentially important roles in regulation of hepatic drug response genes as well as in contributing to gender-related differences in expression of hepatic cytochrome P450s (CYPs) and transcription factors (TFs). PMID: 27264955
26. findings establish a central role for STAT5 activation in the pathogenesis of juvenile myelomonocytic leukemia PMID: 27418650
27. Although inappropriate promoter methylation was not invariantly associated with reduced transcript expression, a significant association was apparent for the ARHGEF4, PON3, STAT5a, and VAX2 gene transcripts (P<0.05). Herein, we present the first genome-wide DNA methylation analysis in a unique HG-NMIBC cohort, showing extensive and discrete methylation changes relative to normal bladder and low-intermediate-grade tumor PMID: 26929985
28. pSTAT5 signaling is implicated in the disease activity of adult and juvenile onset systemic lupus erythematosus PMID: 27041383
29. Data show that fyn proto-oncogene protein (FYN) expression is deregulated in acute myeloid leukemia and that higher expression of FYN, in combination with FLT3 protein-ITD mutation, resulted in enrichment of the STAT5 transcription factor signaling. PMID: 26848862
30. review describes the role of role of STAT5 in immunity and cancer PMID: 26716518
31. We concluded that rs2293157 is an important marker for the therapeutic efficiency of Ara-C-based chemotherapy in patients with AML, especially in the Chinese population. PMID: 26384082
32. Decreased expression of STAT5 was associated with metastases in Colon Carcinoma. PMID: 25773877
33. PTP1B directly regulates STAT5 phosphorylation and its activation via the cAMP/PKA pathway downstream of the 5-HT7 receptor is involved in the suppression of beta-casein expression in MCF-12A cells PMID: 27016479
34. Foxp3 has a rapid turn over in Treg partly controlled at the transcriptional level by the JAK/STAT pathway PMID: 27077371
35. prolactin activation of Stat5 results in the formation of a complex between phospho-Stat5 and BRCA1. Formation of this complex does not interfere with nuclear translocation or binding of BRCA1 to the p21 promoter PMID: 26970274
36. The innate immune regulator STAT-5 is shown to regulate transcription of the ATR binding factor TopBP1, and this is critical for the induction of the ATR pathway in human papillomavirus-infected keratinocytes. PMID: 26695634
37. The two STAT5 isoforms, STAT5a and STAT5b. PMID: 26717567
38. that STAT-5, RUNX-2, and FGFR-2 may have a role in the progression of the mucinous phenotype, in which nuclear STAT-5 may inhibit RUNX-2 prometastatic effect PMID: 26551078
39. Our study suggests that pyrvinium is a useful addition to T-cell lymphoma treatment, and emphasizes the potential therapeutic value of the differences in the mitochondrial characteristics between malignant and normal T-cells in blood cancer. PMID: 26707639
40. support the concept that Jak2-Stat5a/b signaling promotes metastatic progression of prostate cancer by inducing epithelial-to-mesenchymal transition and stem cell properties in prostate cancer cells PMID: 26362718
41. D5 Stat5a plays pathogenic role in breast cancer through, at least partly, increasing trimethylation of the IGFBP-7 promoter region, thereby inhibiting IGFBP-7 expression. PMID: 26387651
42. CD82 regulated BCL2L12 expression via STAT5A and AKT signaling and stimulated proliferation and engrafting of leukemia cells. PMID: 26260387
43. the ETV6/ARG oncoprotein contributes to autonomous cell growth by compensating for the requirement of growth factor through activating STAT5 signaling, which leads to the up-regulation of c-Myc. PMID: 25373509
44. Both Stat5a/b genetic knockdown and antiandrogen treatment induced proteasomal degradation of AR in prostate cancer cells. PMID: 25552366
45. Upregulation of STAT5A is associated with chronic myeloid leukemia. PMID: 25953263
46. High phosphorylated signal transducer and activator of transcription 5 is associated with mammary analogue secretory carcinoma of the salivary gland. PMID: 26252941
47. These data provide the first definitive evidence for a contribution of STAT5a/b to the sex bias in pulmonary hypertension in the hypoxic mouse and implicate reduced STAT5 in the pathogenesis of the human disease. PMID: 25470773
48. STAT5A positively regulates levels of DNMT3A, resulting in inactivation of tumor suppressor genes by epigenetic mechanisms in acute myeloid leukemia cells. PMID: 26059451
49. constitutively active STAT5A(S710F) escapes from SFK-mediated cytoplasmic retention by enhancing STAT5A dimer stability PMID: 25885255
50. The role of STAT proteins, including STAT5, and NF-kappa B in the death of Caco 2 cells incubated with Entamoeba histolytica is reported. PMID: 25352693
顯示更多

收起更多
亞細胞定位：

Cytoplasm. Nucleus. Note=Translocated into the nucleus in response to phosphorylation.
蛋白家族：

Transcription factor STAT family
數(shù)據(jù)庫鏈接：

HGNC: 11366

OMIM: 601511

KEGG: hsa:6776

STRING: 9606.ENSP00000341208

UniGene: Hs.437058

Most popular with customers

YWHAG Recombinant Monoclonal Antibody

Applications: ELISA, WB, IHC

Species Reactivity: Human
YWHAB Recombinant Monoclonal Antibody

Applications: ELISA, WB, IHC, IF, FC

Species Reactivity: Human, Mouse, Rat
Phospho-YAP1 (S127) Recombinant Monoclonal Antibody

Applications: ELISA, WB, IHC

Species Reactivity: Human
XPNPEP1 Recombinant Monoclonal Antibody

Applications: ELISA, IHC, FC

Species Reactivity: Human
XDH Recombinant Monoclonal Antibody

Applications: ELISA, WB, IHC

Species Reactivity: Human
WT1 Recombinant Monoclonal Antibody

Applications: ELISA, WB, IHC

Species Reactivity: Human
WFDC2 Recombinant Monoclonal Antibody

Applications: ELISA, WB, FC

Species Reactivity: Human
WDR5 Recombinant Monoclonal Antibody

Applications: ELISA, IHC, FC

Species Reactivity: Human