LIG1 Recombinant Monoclonal Antibody

說明書

中文名稱：

LIG1重組抗體
貨號：

CSB-RA877322A0HU
規格：

￥1320
圖片：
Western Blot
Positive WB detected in: Hela whole cell lysate, HepG2 whole cell lysate
All lanes: DNA Ligase I antibody at 1:1000
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 102, 89, 99 kDa
Observed band size: 140 kDa

IHC image of CSB-RA877322A0HU diluted at 1:100 and staining in paraffin-embedded human lung cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4℃ overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.

IHC image of CSB-RA877322A0HU diluted at 1:100 and staining in paraffin-embedded human placenta tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4℃ overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
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產品詳情
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靶點詳情

產品詳情

產品描述：

CSB-RA877322A0HU LIG1 Recombinant Monoclonal Antibody 是針對DNA連接酶1（LIG1）開發的高特異性科研用抗體。LIG1是DNA復制與修復的關鍵酶，負責催化DNA鏈末端的連接反應，在維持基因組穩定性和細胞周期調控中發揮重要作用。該抗體通過重組表達技術制備，具有高批次一致性和低交叉反應性，經嚴格驗證適用于ELISA、Western Blot（WB）及免疫組化（IHC）等多種實驗場景。在WB實驗中，抗體可識別天然LIG1蛋白，推薦使用稀釋比例為1:500-1:5000，能清晰檢測目標蛋白表達；IHC實驗推薦稀釋比例為1:50-1:200，可在組織樣本中準確定位LIG1的亞細胞分布。其卓越的性能使其成為研究DNA損傷修復機制、腫瘤發生發展相關信號通路及細胞周期調控的理想工具，可廣泛應用于分子生物學、癌癥研究及遺傳穩定性等基礎科研領域，為解析LIG1在疾病模型中的功能提供可靠支持。
Uniprot No.：

P18858
基因名：

LIG1
別名：

DNA ligase 1 (EC 6.5.1.1) (DNA ligase I) (Polydeoxyribonucleotide synthase [ATP] 1), LIG1
反應種屬：

Human
免疫原：

A synthesized peptide derived from human DNA Ligase I
免疫原種屬：

Homo sapiens (Human)
標記方式：

Non-conjugated
克隆類型：

Monoclonal
抗體亞型：

Rabbit IgG
純化方式：

Affinity-chromatography
克隆號：

7B2
濃度：

It differs from different batches. Please contact us to confirm it.
保存緩沖液：

Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.
產品提供形式：

Liquid
應用范圍：

ELISA, WB, IHC

推薦稀釋比：

Application	Recommended Dilution
WB	1:500-1:5000
IHC	1:50-1:200

Protocols：

ELISA Protocol
Western Blotting (WB) Protocol
Immunohistochemistry (IHC) Protocol
儲存條件：

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
貨期：

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
用途：

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產品評價

靶點詳情

功能：

DNA ligase that seals nicks in double-stranded DNA during DNA replication, DNA recombination and DNA repair.
基因功能參考文獻：
1. single-stranded break repair by human DNA ligase III isoforms reveal biochemical differences from DNA ligase PMID: 28751376
2. A histone H3K9-like mimic within LIG1 is methylated by G9a and GLP and avidly binds UHRF1. Interaction with methylated LIG1 promotes the recruitment of UHRF1 to DNA replication sites and is required for DNA methylation maintenance. PMID: 28803780
3. The rs156641 polymorphism of DNA ligase 1 (LIG1) was significantly associated with lung cancer risk, whereas no association was found between rs3730931/rs439132/rs20579 polymorphisms and lung cancer. PMID: 27352326
4. Data suggest that DNA ligase I (LigI)-deficient 46BR.1G1 cells represent a model to investigate the biological effects of sub-lethal levels of DNA insults. PMID: 26151554
5. The LIG1 CC genotype was associated with susceptibility to non-small cell lung cancer, and the AA genotype demonstrated increased radiosensitivity compared to the AC and CC genotypes. PMID: 26125914
6. A novel method for monitoring functional lesion-specific recruitment of repair proteins in live cells. PMID: 25879709
7. Ppolymorphisms in LIG1 affect its expression and may therefore change its function. PMID: 25189241
8. there is no association between LIGI polymorphisms and cervical cancer risk. However, they may be playing an important role in modulating the risk of cervical adenocarcinoma in North Indian women PMID: 24084463
9. DNA ligase I also interacts with replication factor C, the factor that loads the PCNA trimeric ring onto DNA. PMID: 22918593
10. Data indicate that Ku70/Ku80 facilitates the cooperative binding of multiple XRCC4/Ligase IV (XL) and XLF molecules to DNA. PMID: 23620595
11. Single nucleotide polymorphisms in LIG1 are associated with myelodysplastic syndromes. PMID: 23339595
12. phosphorylation of serine 51 on hLigI plays a critical role in regulating the interaction between hLigI and RFC, which is required for efficient DNA replication and repair. PMID: 22952233
13. Data show that association of study-wide significance (P < 8.2 x 10(-5)) was identified for single-nucleotide polymorphisms (SNP) in TP53, LIG1, and BIK. PMID: 22139380
14. Kinetic mechanism of human DNA ligase I reveals magnesium-dependent changes in the rate-limiting step that compromise ligation efficiency. PMID: 21561855
15. RNA silencing of human DNA ligase I expression severely reduced replication of viral DNA in cells infected with vaccinia virus ligase-deficient mutants. PMID: 20006844
16. DNA ligase I competes with FEN1 to expand repetitive DNA sequences in vitro. PMID: 11948189
17. stimulated by APE1 for progression through the base excision repair pathway PMID: 12200445
18. phosphorylation of DNA ligase I and possibly other replicative enzymes is part of the mechanism that directs the disassembly of the replication machinery at the completion of S-phase. PMID: 12851383
19. majority of single-strand DNA interruptions produced during the repair of alkylated DNA bases are repaired by the pathway mediated by Pol beta and either Lig I or Lig III PMID: 14627836
20. crystal structure of human DNA ligase I (residues 233 to 919) in complex with a nicked, 5' adenylated DNA intermediate PMID: 15565146
21. The human checkpoint sensor and alternative clamp Rad9-Rad1-Hus1 can interact with and specifically stimulate DNA ligase I PMID: 15871698
22. LIG1-deficiency reduces recombinational repair of DNA double-strand beaks. PMID: 15907772
23. human DNA ligase I is stimulated by the Rad9-rad1-Hus1 checkpoint complex PMID: 16731526
24. siRNA mediated down-regulation of DNA ligase I in human HTD114 cells led to impaired end joining that was mediated by 2-, 3- or 10-bp microhomology. PMID: 18440984
25. The LIG1 SNP 5'UTR showed a significant association with glioma risk. PMID: 19124499
26. drastically reduced replicative LigI activity in 46BR.1G1 cells results in the accumulation of both single-stranded and double-stranded DNA breaks. PMID: 19223467
27. a mutant version of hLigI, which mimics the hyperphosphorylated M-phase form of hLigI, does not interact with and is not inhibited by RFC, demonstrating that inhibition of ligation is dependent upon the interaction between hLigI and RFC PMID: 19223468
28. The DNA binding domain (DBD) within the hLigI catalytic fragment interacts with both PCNA and the heterotrimeric cell-cycle checkpoint clamp, hRad9-hRad1-hHus1 (9-1-1). PMID: 19523882
29. Data support the notion that DNA ligase I participates in homology dependent pathways that deal with replication-associated lesions generated when replication fork encounters DNA damage. PMID: 19597347
30. Data show that disruption of LigI and PCNA interactions influences trinucleotide repeat instability. PMID: 19628465
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亞細胞定位：

Nucleus.
蛋白家族：

ATP-dependent DNA ligase family
數據庫鏈接：

HGNC: 6598

OMIM: 126391

KEGG: hsa:3978

STRING: 9606.ENSP00000263274

UniGene: Hs.1770

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