CDC25B Recombinant Monoclonal Antibody

說明書

中文名稱：

CDC25B重組抗體
貨號：

CSB-RA004995A0HU
規格：

￥1320
其他：

產品詳情
產品評價
相關產品
靶點詳情

產品詳情

產品描述：

本品為針對CDC25B靶點開發的重組單克隆抗體，適用于ELISA檢測。CDC25B作為細胞周期調控的核心因子，通過去磷酸化作用激活CDK1/cyclin B復合體，驅動細胞有絲分裂進程，其異常表達與腫瘤細胞增殖及基因組不穩定性密切相關。該抗體經ELISA平臺嚴格驗證，具有高親和力與靶標特異性，能夠精準識別天然構象下的CDC25B蛋白，為細胞周期動力學研究提供可靠工具。適用于探索細胞周期調控機制、腫瘤發生發展相關通路研究以及抗癌藥物靶點驗證等科研領域。本產品采用重組表達技術生產，具有高批次穩定性與低交叉反應性，支持體外實驗中對CDC25B蛋白的定量分析與功能研究，滿足基礎科研中對靶蛋白檢測的標準化需求。
Uniprot No.：

P30305
基因名：

CDC25B
別名：

AI604853 antibody; Cdc 25B antibody; Cdc25b antibody; CDC25HU2 antibody; Cdc25m2 antibody; Cell division cycle 25 homolog B antibody; Cell division cycle 25B antibody; Cell division cycle 25B isoform 1 antibody; Cell division cycle 25B isoform 2 antibody; Cell division cycle 25B isoform 3 antibody; Cell division cycle 25B isoform 4 antibody; Cell division cycle 25B isoform 5 antibody; Dual specificity phosphatase Cdc25B antibody; EC 3.1.3.48 antibody; M phase inducer phosphatase 2 antibody; M-phase inducer phosphatase 2 antibody; MPIP2_HUMAN antibody
反應種屬：

Human
免疫原：

A synthesized peptide derived from human CDC25B
免疫原種屬：

Homo sapiens (Human)
標記方式：

Non-conjugated
克隆類型：

Monoclonal
抗體亞型：

Rabbit IgG
純化方式：

Affinity-chromatography
克隆號：

3D6
濃度：

It differs from different batches. Please contact us to confirm it.
保存緩沖液：

Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.
產品提供形式：

Liquid
應用范圍：

ELISA
Protocols：

ELISA Protocol
儲存條件：

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
貨期：

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
用途：

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產品評價

靶點詳情

功能：

Tyrosine protein phosphatase which functions as a dosage-dependent inducer of mitotic progression. Required for G2/M phases of the cell cycle progression and abscission during cytokinesis in a ECT2-dependent manner. Directly dephosphorylates CDK1 and stimulates its kinase activity. The three isoforms seem to have a different level of activity.
基因功能參考文獻：
1. miR-152 was a tumor suppressor in EC that inhibited proliferation of human endometrial cancer cells via inducing G2/M phase arrest by suppressing CDC25B expression. PMID: 29353204
2. High CDC25B expression is associated with non-small cell lung cancer metastasis. PMID: 28656293
3. YWHAE silencing induces cell proliferation, invasion and migration through the up-regulation of CDC25B and MYC in gastric cancer cells. PMID: 27863420
4. While the low expression level of DUSP7 was restricted to patients with positive rheumatoid factor and anti-citrullinated protein antibodies, the altered expression of CDC25B correlated with the activity of early arthritis. PMID: 28253537
5. Conformational flexibility of the complete catalytic domain of Cdc25B phosphatase has been demonstrated. PMID: 27410025
6. High CDC25B expression is associated with esophageal carcinoma. PMID: 25775393
7. Solution NMR studies reveal no global flexibility in the structure of CDC25b catalytic domain PMID: 24740794
8. For the first time, we demonstrate that miRNA-211 is a direct negative regulator of CDC25B expression in TNBC cells, alters other related target proteins CCNB1 and FOXM1, and then inhibits breast cancer cells growth, migration, and invasion PMID: 25680404
9. FK-3000 exerts its antiproliferative effect through G2/M cell cycle arrest via downregulation of cyclin B and phospho-CDC2 by p38 MAPK phosphorylation and CDC25B dephosphorylation . PMID: 25384584
10. RSK promotes G2/M transition in mammalian cells through activating phosphorylation of Cdc25A and Cdc25B. PMID: 23708659
11. Cdc25B upregulation and 14-3-3sigma downregulation might promote development of bladder cancer and suggested a poor prognosis. PMID: 24234332
12. Positive expression of CDC25B in astrocytoma affects the prognosis in an adverse manner. PMID: 24344030
13. CDC25B is a pro-influenza A virus host factor. PMID: 24109234
14. CDC25B, through activation of a centrosomal pool of CDK2, stabilises the local pool of Mps1 which in turn regulates the level of centrin 2 at the centrosome. PMID: 23840880
15. Phosphorylation and inhibition of cdc25B causes delayed progression from G2 into mitosis in melanoma cells. PMID: 23908401
16. Indirubin, an acting component of indigo naturalis, inhibits EGFR activation and EGF-induced CDC25B gene expression in epidermal ke PMID: 22721997
17. downregulation of Cdc25B resulted in slower growth, more G2/M cells, weaker capacity for migration and invasion and induction of apoptosis in renal carcinoma cell line 769-P transfectants; findings suggest an important role of Cdc25B in renal cell carcinoma development PMID: 22631674
18. Transcriptional repression mediated by IER5 regulates Cdc25B expression levels via the release of NF-YB and p300 in acute myeloid leukemia. PMID: 22132193
19. We identified CDC25B as novel miR-148a target which may confer a proliferative advantage in pancreatic ductal adenocarcinomas. PMID: 21709669
20. Ser169 phosphorylation can disrupt 14-3-3 binding to Ser151 activating Cdc25B3, providing a mechanism for regulating Cdc25B3 activation without dephosphorylation of the 14-3-3 binding sites. PMID: 21558810
21. there are 13 sites phosphorylated by PLK1 in CDC25B.This study illustrates the complexity of the phosphorylation pattern and of the subsequent regulation of CDC25B activity. PMID: 21640712
22. Deletion and mutation analyses of the Cdc25B promoter revealed that downregulation by p53 is dependent on the presence of functional Sp1/Sp3 and NF-Y binding sites PMID: 21242964
23. CDC25B forms a close association with Ctn-2 proteins at the centrosome. PMID: 21091437
24. Our findings reveal the existence of a previously unrecognized CDC25B isoform that operates specifically in the nucleus to reinitiate G2/M transition after DNA damage PMID: 21363925
25. The mitotic phosphorylation of Ser(321) acts to maintain full activation of Cdc25B by disrupting 14-3-3 binding to Ser(323) and enhancing the dephosphorylation of Ser(323) to block 14-3-3 binding to this site. PMID: 20801879
26. CDC25B a minor role in the pathogenesis and/or progression of vulvar squamous cell carcinomas. PMID: 20500813
27. A moderate and unscheduled increase in CDC25B level, as observed in a number of human tumours, is sufficient to overcome the S-phase checkpoint efficiency thus leading to replicative stress and genomic instability. PMID: 20128929
28. A new mechanism of CDC25B regulation in response to stress. PMID: 20176018
29. Adventitious arsenate reductase activity of the catalytic domain of the human Cdc25B phosphatases. PMID: 20025242
30. nuclear localization and serine 146 integrity required for induction of mitosis PMID: 12107172
31. pEg3 kinase is able to specifically phosphorylate CDC25B in vitro. One phosphorylation site was identified and corresponded to serine 323 PMID: 12400006
32. we demonstrate that phosphorylation of CDC25B by protein kinase CK2 increases the catalytic activity of the phosphatase in vitro as well as in vivo.CDC25B interacts with CK2, and this interaction, mediated by the CK2beta regulatory subunit PMID: 12527891
33. upregulation of Cdc25B in human prostate cancer and its interplay with androgen receptor may contribute to prostate cancer development PMID: 12569365
34. Data show that FHL3 (human four-and-a-half LIM-only protein 3) interacts with human phosphatase CDC25B in the cell nucleus. PMID: 12681290
35. Cdc25B may play important roles in the development and progression of endometrioid endometrial carcinoma and clear cell carcinoma and uterine pappillary serous carcinoma by different mechanism. PMID: 14559803
36. cdc25B plays a role in the early phase of thyroid lymphoma possibly including the malignant transformation from chronic thyroiditis PMID: 14767575
37. Identification of a nuclear export signal in CDC25B. PMID: 15003534
38. Phosphorylation of CDC25B by AURA at the centrosome contributes to the G2-M transition. PMID: 15128871
39. 14-3-3 subtypes can control the subcellular localization of CDC25B by binding to a specific site. PMID: 15173315
40. Cdc25B localisation and nuclear export changes during the cell cycle and in response to stress PMID: 15456846
41. Results suggest that cdc25B is important especially in the early phase of breast carcinoma progression. PMID: 15550849
42. These results support a model in which pEg3 contributes to the control of progression through mitosis by phosphorylation of the CDC25 phosphatases. PMID: 15908796
43. Aurora-A-mediated phosphorylation of CDC25B at the centrosome is an important step contributing to the earliest events inducing mitosis, upstream of CDK1-cyclin B1 activation PMID: 16082213
44. MAPKAP kinase-2 phosphorylates CDC25B on multiple sites including S169, S323, S353 and S375, while p38SAPK phosphorylates CDC25B on S249. PMID: 16861915
45. Results show under normal cell cycle conditions and in the absence of DNA damage, CDC25B is constitutively phosphorylated by CHK1 during interphase and thus prevents the premature initiation of mitosis. PMID: 17003105
46. Chk1 functions to coordinate mitotic events through regulation of Cdc25B PMID: 17106257
47. The expression of CDC25B in different cellular compartments of human spermatozoa suggests that there are diverse noncell-cycle-related functions of CDC25B in terminally differentiated human germ cells. PMID: 17336969
48. p38 regulates the timing of mitotic entry via modulation of Cdc25B activity under normal nonstress conditions PMID: 17548358
49. Cdc25B, but not Cdc25C, is capable of inhibiting cellular proliferation in a manner dependent upon its catalytic activity PMID: 17591782
50. CDC25B splice variants have differential mitotic stabilities, a feature that is likely to have major consequences on the local control of cyclin-dependent kinase-cyclin activities during mitotic progression. PMID: 17599046
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亞細胞定位：

Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Cytoplasm, cytoskeleton, spindle pole.
蛋白家族：

MPI phosphatase family
數據庫鏈接：

HGNC: 1726

OMIM: 116949

KEGG: hsa:994

STRING: 9606.ENSP00000245960

UniGene: Hs.153752

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