BCHE Recombinant Monoclonal Antibody

說明書

中文名稱：

BCHE重組抗體
貨號：

CSB-RA252650A0HU
規格：

￥1320
圖片：
Western Blot
Positive WB detected in: 293 whole cell lysate
All lanes: BCHE antibody at 1:2000
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 69 kDa
Observed band size: 90 kDa
其他：

產品詳情
產品評價
相關產品
靶點詳情

產品詳情

產品描述：

CSB-RA252650A0HU 重組單克隆抗體靶向丁酰膽堿酯酶（BCHE），該蛋白是一種廣泛分布于血液、肝臟及神經組織的絲氨酸水解酶，主要負責水解膽堿酯類物質（如琥珀酰膽堿）及部分酯類麻醉劑，在脂質代謝和解毒過程中發揮重要作用。本抗體經重組表達技術制備，在ELISA與Western Blot實驗中展現出高靈敏度和特異性，Western Blot推薦使用1:500至1:5000稀釋度，可清晰識別天然及重組BCHE蛋白。經多批次驗證，該抗體在不同種屬來源的組織樣本（如人肝臟組織裂解液）中均能穩定檢測目標蛋白，且批間一致性優異。適用于探究BCHE在膽堿能信號傳導、脂質代謝調控及毒物解毒過程中的分子機制，可支持神經退行性疾病、代謝綜合征等科研模型的蛋白質表達分析，為相關疾病的分子病理研究及藥物靶點探索提供可靠工具。
Uniprot No.：

P06276
基因名：

BCHE
別名：

Cholinesterase (EC 3.1.1.8) (Acylcholine acylhydrolase) (Butyrylcholine esterase) (Choline esterase II) (Pseudocholinesterase), BCHE, CHE1
反應種屬：

Human
免疫原：

A synthesized peptide derived from human BCHE
免疫原種屬：

Homo sapiens (Human)
標記方式：

Non-conjugated
克隆類型：

Monoclonal
抗體亞型：

Rabbit IgG
純化方式：

Affinity-chromatography
克隆號：

1E9
濃度：

It differs from different batches. Please contact us to confirm it.
保存緩沖液：

Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.
產品提供形式：

Liquid
應用范圍：

ELISA, WB

推薦稀釋比：

Application	Recommended Dilution
WB	1:500-1:5000

Protocols：

ELISA Protocol
Western Blotting (WB) Protocol
儲存條件：

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
貨期：

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
用途：

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產品評價

靶點詳情

功能：

Esterase with broad substrate specificity. Contributes to the inactivation of the neurotransmitter acetylcholine. Can degrade neurotoxic organophosphate esters.
基因功能參考文獻：
1. An increased frequency of the BChE K-allele in multiple sclerosis patients as compared to controls was found. PMID: 29358722
2. This study validated preoperative serum BChE levels as an independent prognostic factor for urinary tract urothelial carcinoma after radical nephroureterectomy. PMID: 29177431
3. we present and examine means of manipulating brain BChE levels by viral gene transfer, either regionally or globally, to modulate ghrelin signaling for long-term therapeutic purposes and to set the stage for exploring the neurophysiological impact of such an intervention. PMID: 28698452
4. The BChE-K polymorphisms are associated with deleterious changes in cognitive decline in MCI patients treated with donepezil for 3 years. PMID: 27911294
5. BCHE-K* positive subjects (and APOE-epsilon4) display an earlier age of onset of Alzheimer's Disease and an accelerated cognitive decline. PMID: 27567841
6. Discovery of potent carbonic anhydrase, acetylcholinesterase, and butyrylcholinesterase enzymes inhibitors: The new amides and thiazolidine-4-ones synthesized on an acetophenone base.() PMID: 28544359
7. HuBChE sequesters OP nerve agent in the bloodstream preventing the nerve agent from reaching critical target organ systems. HuBChE was effective when used as both a pre-treatment and as a post-exposure therapy. PMID: 28225154
8. Significance of BChE Genetic Variants to Risk of Toxicity from Cholinesterase Inhibitors (review) PMID: 27551784
9. Age, sex or smoking status did not influence butyrylcholinesterase activity in a healthy population. PMID: 28465191
10. Mutations in the butyrylcholinesterase gene were associated with prolonged effect of succinylcholine or mivacurium. PMID: 27031121
11. prolonged apnea after suxamethonium came about as a result of butyrylcholinesterase deficiency or mutation or acquired conditions leading to a decrease in the plasma cholinesterase activity PMID: 26439437
12. the molecular mechanisms by which point mutations may lead to silent BChE variant or alter catalytic activity, is reported. PMID: 27062896
13. BChE expression might be regulated by alpha-linolenic acid in HepG2 cells. PMID: 27106529
14. Nine out of 18 (50%) individuals with butyrylcholinesterase activity below 2000 U/L had a mutation in 5'UTR (32G/A), intron 2 (c.1518-121T/C) or exon 4 (c.1699G/A; the K variant mutation). PMID: 27109752
15. No significant difference in BChE levels of healthy pregnant women and high-risk pregnant women who had undergone a cesarean section under general anesthesia. PMID: 26955768
16. Association between polymorphisms of BCHE and vitiligo in a two-step genetic association study. Upon confirmation of genetic association, the effect was investigated by a genotype-phenotype correlation experiment. PMID: 26189613
17. Low serum Butyrylcholinesterase levels are associated with recurrence in prostate cancer. PMID: 26223693
18. BCHE-K is associated with a reduced risk for Alzheimer disease and Lewy body dementia whereas APOEvarepsilon4 is associated with more rapid cognitive decline. PMID: 26757188
19. Low BChE activity as a predictor of mortality in acute myocardial infarction might be related to its association with poor cardiac function. PMID: 27468571
20. rs1803274 polymorphism of the BCHE gene represents a risk factor for in-stent restenosis after percutaneous coronary intervention. PMID: 26497592
21. Complement component C3 and butyrylcholinesterase activity are associated with neurodegeneration and clinical disability in multiple sclerosis. PMID: 25835709
22. BChE is a strong predictor for cardiac mortality specifically in younger patients with acute coronary syndrome aged between 45 and 64 years. PMID: 25933219
23. High resolution melting analysis for the butyrylcholinesterase atypical variant genotyping is a simple, rapid, sensitive and low cost method PMID: 25336127
24. There is a link between systemic inflammatory markers, oxidative stress, the PON192rs662-Q allele and BuChE-non-UU-phenotype and their corresponding enzymatic activity which may be considered as a risk factor for the severity of rheumatoid arthritis. PMID: 25179377
25. There is a significant decrease in serum ChE activity after severe trauma PMID: 25471831
26. This study proves that the p.Ala34Val BChE variant determines the "silent" phenotype (unable to hydrolyze succinylcholine and mivacurium). PMID: 25264279
27. Data indicate that butyrylcholinesterase (BChE) was significantly associated with overall survival (OS). PMID: 24951323
28. analysis of the T920C mutation allele frequency in butyrylcholinesterase gene in an Indian population PMID: 25447891
29. Plasma arylesterase activity is positively associated with plasma ChE specific activity in a nested case-control study. PMID: 24473115
30. BChE-K carriers are protected from the pathological detriments of Alzheimer's disease that affect cortical thickness. PMID: 24479631
31. results confirm the association of APOE with Abeta deposition and represent the largest known effect of BCHE on an AD-related phenotype. PMID: 23419831
32. Results indicate that BuChE non-UU phenotype and PON 55M allele are significant risk factors for systemic lupus erythematosus. PMID: 24399815
33. Our findings suggest that the AA genotype of rs1803274 is a risk factor for crack cocaine use, which is more addictive than powder cocaine use. PMID: 24312228
34. We report the case of a 65-year-old patient who was diagnosed with butyrylcholinesterase resulting in prolonged paralysis and extended mechanical ventilation PMID: 24446003
35. Two mutants of human BCHE can effectively eliminate (-)-cocaine, cocaethylene and norcocaine in simplified kinetic models of cocaine abuse and overdose associated with the concurrent use of cocaine and alcohol. PMID: 24870023
36. BChE activity might be involved in the pathogenesis of preeclampsia through influence on lipid and lipoprotein metabolism and oxidative stress. PMID: 23650977
37. The present data are still inconclusive with respect to a possible contribution of common genetic variants in BCHE to the pathogenesis of attention-deficit/hyperactivity syndrome. PMID: 24041656
38. CocH3 (BCHE mutant) with an identical concentration with that of the endogenous BChE in human plasma can effectively eliminate both cocaine and norcocaine in a simplified kinetic model of cocaine abuse. PMID: 24125115
39. BCHE 1914G allele showed higher frequency in the obese group. Carriers of 1914G BCHE allele showed lower mean BChE activity.BCHE 1914G allele is influencing physiological mechanisms related to obesity. PMID: 24001779
40. Data indicate that high AChE affinity of the compounds was achieved by optimizing different substituents on the pyridazinone ring, without sacrificing the AChE/BuChE selectivity profile. PMID: 23466605
41. Water in the active site gorge of the D70G mutant is more easily depleted than that in wild-type BChE. PMID: 23782236
42. These findings show that BCHE can hydrolyze 2-Arachidonoylglycerol which may be evidence of a more specific role for BCHE in endocannabinoid regulation. PMID: 23689009
43. This review examines the roles of gender and BuChE genotype in the phenotypic expression of Alzheimer disease[review] PMID: 22402324
44. Elevated levels of BuChE observed in active multiple sclerosis (MS) lesions could be related to the decompaction of myelin characteristic of the disorder. PMID: 22778864
45. [review] Butyrylcholinesterase has diverged both structurally and in terms of tissue and cellular expression patterns from acetylcholinesterase. PMID: 22750491
46. aspirin is hydrolyzed in plasma by two enzymes, BChE and a new extracellular form of platelet-activating factor acetylhydrolase, PAFAH1b2. PMID: 23508960
47. characterized the catalytic activities of wild-type BChE and the A199S/F227A/S287G/A328 W/Y332G mutant against both (+)- and (-)-cocaine at the same time under the same experimental conditions PMID: 22917637
48. verify amplification and/or deletion in the ACHE, BCHE, EPHB4 and MME genes in 32 samples of sporadic breast cancer PMID: 23063927
49. performed molecular dynamics simulation to probe the structural stability of Indian variant (L307P) in comparison with wild and other BChE variants (D70G, E497V, V142M) having differential esterase activity PMID: 23123771
50. Alkylating molecules were synthesized and the crystal structures they form with soman-aged butyrylcholinesterase were solved. PMID: 22922115
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相關疾病：

Butyrylcholinesterase deficiency (BChE deficiency)
亞細胞定位：

Secreted.
蛋白家族：

Type-B carboxylesterase/lipase family
組織特異性：

Detected in blood plasma (at protein level). Present in most cells except erythrocytes.
數據庫鏈接：

HGNC: 983

OMIM: 177400

KEGG: hsa:590

STRING: 9606.ENSP00000264381

UniGene: Hs.420483

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