ROBO1 Recombinant Monoclonal Antibody

說明書

中文名稱：

ROBO1重組抗體
貨號：

CSB-RA896760MA1HU
規格：

￥1320
圖片：
IHC image of CSB-RA896760MA1HU diluted at 1:30 and staining in paraffin-embedded human brain tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-Mouse IgG labeled by HRP and visualized using 0.05% DAB.

IHC image of CSB-RA896760MA1HU diluted at 1:30 and staining in paraffin-embedded human gastric cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-Mouse IgG labeled by HRP and visualized using 0.05% DAB.

Immunofluorescence staining of A549 cell with CSB-RA896760MA1HU at 1:20, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

Immunofluorescence staining of Hela cell with CSB-RA896760MA1HU at 1:20, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

Overlay Peak curve showing Hela cells surface stained with CSB-RA896760MA1HU (red line) at 1:50. Then 10% normal goat serum was Incubated to block non-specific protein-protein interactions followed by the antibody (1μg/1*10⁶cells) for 45 min at 4°C. The secondary antibody used was FITC-conjugated Goat Anti-Mouse IgG(H+L) at 1/200 dilution for 35 min at 4°C. Isotype control antibody (green line) was mouse IgG1 (1μg/1*10⁶cells) used under the same conditions. Acquisition of >10,025 events was performed.
其他：

產品詳情
產品評價
相關產品
靶點詳情

產品詳情

產品描述：

ROBO1重組單克隆抗體（產品編號：CSB-RA896760MA1HU）靶向Slit-Robo信號通路關鍵受體ROBO1，該蛋白在軸突導向、細胞遷移及血管生成等生物學過程中發揮重要作用，其異常表達與腫瘤微環境調控及神經系統發育密切相關。本抗體經ELISA、免疫組化（IHC）、免疫熒光（IF）和流式細胞術（FC）嚴格驗證，推薦使用稀釋比例為1:20-1:200，展現出優異的批次穩定性和靶標結合特異性。在IHC實驗中可清晰檢測石蠟包埋組織中的抗原定位，IF實驗適用于細胞固定樣本的亞細胞結構觀察，FC則能有效分析細胞表面ROBO1表達水平，同時支持ELISA平臺的高靈敏度定量檢測。該產品適用于神經發育機制、腫瘤侵襲遷移研究及血管生成調控等領域的科研探索，為體外實驗提供可靠工具，例如通過組織切片染色解析蛋白分布特征，或結合流式技術定量評估細胞群體中ROBO1的動態表達變化。
Uniprot No.：

Q9Y6N7
基因名：

ROBO1
別名：

Deleted in U twenty twenty antibody; DUTT 1 antibody; DUTT1 antibody; FLJ21882 antibody; H Robo 1 antibody; H-Robo-1 antibody; hRobo 1 antibody; Robo 1 antibody; Robo1 antibody; ROBO1_HUMAN antibody; Roundabout 1 antibody; Roundabout axon guidance receptor homolog 1 antibody; Roundabout homolog 1 antibody; Roundabout homolog1 precurser antibody; Roundabout1 antibody; SAX 3 antibody; SAX3 antibody
反應種屬：

Human
免疫原：

Recombinant Human ROBO1 protein
免疫原種屬：

Homo sapiens (Human)
標記方式：

Non-conjugated
克隆類型：

Monoclonal
抗體亞型：

Mouse IgG
純化方式：

Affinity-chromatography
克隆號：

22D5
濃度：

It differs from different batches. Please contact us to confirm it.
保存緩沖液：

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
產品提供形式：

Liquid
應用范圍：

ELISA, IHC, IF, FC

推薦稀釋比：

Application	Recommended Dilution
IHC	1:20-1:200
IF	1:20-1:200
FC	1:20-1:200

Protocols：

ELISA Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol
Flow cytometry (FC) Protocol
儲存條件：

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
貨期：

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
用途：

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產品評價

靶點詳情

功能：

Receptor for SLIT1 and SLIT2 that mediates cellular responses to molecular guidance cues in cellular migration, including axonal navigation at the ventral midline of the neural tube and projection of axons to different regions during neuronal development. Interaction with the intracellular domain of FLRT3 mediates axon attraction towards cells expressing NTN1. In axon growth cones, the silencing of the attractive effect of NTN1 by SLIT2 may require the formation of a ROBO1-DCC complex. Plays a role in the regulation of cell migration via its interaction with MYO9B; inhibits MYO9B-mediated stimulation of RHOA GTPase activity, and thereby leads to increased levels of active, GTP-bound RHOA. May be required for lung development.
基因功能參考文獻：
1. Robo1 expression might counteract migration and also radiation-induced migration of glioblastoma cells, a process that might be connected to mesenchymal-epithelial transition. PMID: 29864155
2. the expression of hsa_circRNA0054633 has a protective effect against high glucoseinduced endothelial cell dysfunction by targeting ROBO1 and HO1. PMID: 29693114
3. Overexpression of miR-218 and inhibition of Robo1 reduced the number of the invased cells of HCC4006. PMID: 28738960
4. miR-218 inhibits human lung adenocarcinoma cell migration and invasion via the suppression of Ecop and Robo1 expression PMID: 28936884
5. we show that variants in ROBO1 are associated with CHD involving septal defects and tetralogy of Fallot. PMID: 28592524
6. SLIT2/ROBO1 signalling may regulate trophoblast differentiation and invasion causing restricting beta human chorionic gonadotrophin (beta-hCG) production, shallow trophoblast invasion and inhibiting placental angiogenesis in missed and threatened miscarriage during the first trimester. PMID: 28485101
7. The findings indicate that the migration of human neural progenitor cells from the fetal subventricular zone to the olfactory bulb is partially regulated by the Slit2-Robo1 axis. PMID: 28406573
8. These findings offer direct evidence supporting ROBO1-callosum association in humans and also provide valuable insight into the functions of ROBO1 and the gene-to-brain mechanisms that underlie human reading. PMID: 28240421
9. Differential expression levels and methylation status of ROBO1 in mantle cell lymphoma and chronic lymphocytic leukaemia. PMID: 28004534
10. The alteration of Slit2 and Robo1 expression in the retinas of diabetic rats and patients with proliferative diabetic retinopathy suggests a role for the Slit-Robo signal in the various stages diabetic retinopathy. PMID: 28973045
11. Results demonstrate that human ROBO1 may be involved in the regulation of the structure and connectivity of posterior part of corpus callosum. PMID: 27240594
12. ROBO1 gene mutation is responsible for the development of pituitary stalk interruption syndrome. PMID: 28402530
13. human placental multipotent mesenchymal stromal cell express Slit2 and both Robo1 and Robo4 are present in human umbilical vein endothelial cells. PMID: 26745454
14. Slit2-Robo1 signaling promoted the adhesion, invasion and migration of tongue carcinoma cells by upregulating the expression levels of MMP2 and MMP9 and, downregulating the expression of E-cadherin. PMID: 27431199
15. High Slit2 expression is associated with glioma. PMID: 27916173
16. ROBO1 mediated the inhibitory effect of miR-218 on angiogenesis in gastric cancer. PMID: 28323002
17. Results indicate the importance of SLIT2-ROBO1-CDC42 signaling pathway in predicting tumor progression. PMID: 27659325
18. We postulate that Robo1 promotes tumor invasion partly by the upregulation of MMP2 after activation of PI3K/Akt signaling pathway. Notably, Slit2 knockdown caused the upregulation of Robo1 expression both at the mRNA and protein levels. Thus, the stimulatory effects of Slit2 knockdown on tumor progression can be ascribed, at least in part, to the upregulation of Robo1 and its positive role in tumor progression. PMID: 27176045
19. ROBO1 deletion in a putative transcriptional regulatory region PMID: 26427657
20. Over-expression of miR-218 in glioma cells may inhibit the proliferation and tumorigenicity through targeting Robo1, suggesting that miR-218 could be a potential target for developing therapies in treating glioma. PMID: 26889813
21. Significantly increased serum Slit2 levels and hepatic expression of Slit2 and Robo1 were observed in patients with liver fibrosis. PMID: 26264936
22. expression pattern in extravillous trophoblasts associated with the remodeling events of the tubal pregnancy PMID: 26282852
23. our findings indicated that Slit2/Robo1 axis possibly be regarded as a significant clinical parameter for predicting brain metastasis in breast cancer patients. PMID: 26400100
24. miR-29a markedly inhibits the protein expression of Robo1 in mesenchymal stem cells. PMID: 26252416
25. A SLIT2/ROBO1 signaling circuit as a key regulatory mechanism. PMID: 26975850
26. These results suggest that Slit2/Robo1 binding exerts an effect on cell migration, which is negatively regulated by Robo4, and Robo1 may function by interacting with CdGAP in HUVECs. PMID: 26713366
27. ROBO1 somatic mutation is associated with myelodysplastic syndrome progression. Overexpression of ROBO1 produces anti-proliferative and pro-apoptotic effects in leukaemia cells. However, this effect was lost in ROBO mutants. PMID: 26608094
28. Robo1 promoted cell division cycle 42 (Cdc42) expression in HUVECs, and a distorted actin cytoskeleton in HUVECs was observed when Robo1 expression was suppressed. In conclusion, Robo1 promoted angiogenesis in HCC mediated by Cdc42. PMID: 26022159
29. these studies demonstrate that miR-219-5p inhibited cancer cell growth and invasion by direct targeting ROBO1, implicating miR-219-5p as an attractive candidate for cancer therapy. PMID: 26081620
30. Slit2/Robo1 signaling promotes intestinal tumorigenesis through Src-mediated activation of the Wnt/beta-catenin pathway. PMID: 25605242
31. miR29a inhibits cell migration and invasion in breast cancer cells, at least in part, by directly targeting Robo1. PMID: 25955714
32. In this review, we summarize recent findings demonstrating that the neuronal guidance cues, Slit and Roundabout (Robo), prevent the migration of multiple leukocyte subsets towards diverse inflammatory chemoattractants. PMID: 24777535
33. Inactivation of SLIT2 and/or ROBO1 is one of the early events in development of dysplastic lesions of head and neck and has prognostic importance. PMID: 25465073
34. identify two separate binding sites for heparin interaction with Robo1: one binding site at the previously identified site for heparin dp8 and a second binding site at the N terminus of Robo1 that is disordered in the x-ray crystal structure PMID: 25752613
35. prognostic implications of SLIT and ROBO1 expression in gallbladder cancer PMID: 24777813
36. Data show that ubiquitin specific peptidase 33 (USP33) mediates nerve tissue proteins Slit-Robo signaling in lung cancer cell migration. PMID: 24981056
37. ROBO1 was a functional target of miRNA-218's downstream pathway involving in cell invasion and migration of pancreatic cancer. PMID: 25010661
38. ROBO1 contributs to the deficits in developmental dyslexia and its correlated phenotypes. PMID: 24430574
39. Family-based analysis shows association of SNPs in ROBO1 with reading diasbilities. PMID: 24612512
40. Lower expression of ROBO1 is associated with prostate cancer disease progression. PMID: 24752651
41. Full-length Robo1 is present almost exclusively as a dimer; parallel studies demonstrate the biological activity of Slit2 and its interaction with Robo1. PMID: 24673457
42. Frameshift mutations of ROBO1 and ROBO2 genes and alteration of ROBO2 expression in gastric and colorectal cancers suggest that both genes might play roles in the pathogenesis of both cancers. PMID: 24247621
43. Downregulation of miRNA-218 and upregulation of ROBO-1 were first demonstrated in pancreatic cancer. PMID: 23733161
44. Low Robo1 expression was associated with cell proliferation and migration in ICC and was one of the adverse prognostic factors in patients with these tumors. PMID: 23953227
45. No genetic association of ROBO1 with developmental dyslexia was found in Indian population. PMID: 23954868
46. Data indicate that slit2N alters the localization and binding of Robo1 to WASp and LSP1 in HIV-1-gp120-treated immature dendritic cells (iDCs). PMID: 23119100
47. breast cancer cell migration and invasion was promoted when miRNA- 218 was significantly down regulated, in the way of up-regulation of Robo1. PMID: 22898079
48. data suggests the importance of abrogation of SLIT2-ROBO1 and SLIT2-ROBO2 interactions in the initiation and progression of CACX and also for early diagnosis and prognosis of the disease PMID: 22719878
49. we report that the tumorigenic potential of breast cancer cells is determined by an interaction between the Robo1 receptor and its ligand Slit2 PMID: 22826604
50. Robo1 expression correlates negatively with invasive ductal carcinoma brain metastasis, and correlates positively with the age and prognosis of IDC patients. PMID: 21875486
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亞細胞定位：

Cell membrane; Single-pass type I membrane protein. Cell projection, axon. Endoplasmic reticulum-Golgi intermediate compartment membrane; Single-pass membrane protein.
蛋白家族：

Immunoglobulin superfamily, ROBO family
組織特異性：

Widely expressed, with exception of kidney.
數據庫鏈接：

HGNC: 10249

OMIM: 602430

KEGG: hsa:6091

STRING: 9606.ENSP00000420321

UniGene: Hs.744218

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