NOS1 Recombinant Monoclonal Antibody

說明書

中文名稱：

NOS1 Recombinant Monoclonal Antibody
貨號：

CSB-RA580392A0HU
規格：

￥1320
圖片：
Immunofluorescence staining of Hela cell with CSB-RA580392A0HU at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Overlay Peak curve showing U251 cells stained with CSB-RA580392A0HU (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100 for?10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*10⁶cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 35min at 4℃.Control antibody (green line) was Rabbit IgG (1ug/1*10⁶cells) used under the same conditions. Acquisition of >10,000 events was performed.
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靶點詳情

產品詳情

Uniprot No.：

P29475
基因名：

NOS1
別名：

2310005C01Rik antibody; BNOS antibody; Constitutive NOS antibody; EC 1.14.13.39 antibody; IHPS 1 antibody; IHPS1 antibody; N-NOS antibody; NC-NOS antibody; neuronal Nitric Oxide Synthase antibody; Neuronal NOS antibody; Nitric oxide synthase ; neuronal; included antibody; Nitric oxide synthase 1 (neuronal) antibody; Nitric oxide synthase 1 antibody; Nitric oxide synthase; brain antibody; Nitric oxide synthase; penile neuronal; included antibody; NNOS antibody; NO antibody; NOS 1 antibody; NOS antibody; NOS type I antibody; NOS-I antibody; NOS1 antibody; NOS1_HUMAN antibody; Peptidyl-cysteine S-nitrosylase NOS1 antibody
反應種屬：

Human
免疫原：

A synthesized peptide derived from human NOS1
免疫原種屬：

Homo sapiens (Human)
標記方式：

Non-conjugated
克隆類型：

Monoclonal
抗體亞型：

Rabbit IgG
純化方式：

Affinity-chromatography
克隆號：

3D1
濃度：

It differs from different batches. Please contact us to confirm it.
保存緩沖液：

Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.
產品提供形式：

Liquid
應用范圍：

ELISA, IF, FC

推薦稀釋比：

Application	Recommended Dilution
IF	1:50-1:200
FC	1:50-1:200

Protocols：

ELISA Protocol
Immunofluorescence (IF) Protocol
Flow cytometry (FC) Protocol
儲存條件：

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
貨期：

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
用途：

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產品評價

靶點詳情

功能：

Produces nitric oxide (NO) which is a messenger molecule with diverse functions throughout the body. In the brain and peripheral nervous system, NO displays many properties of a neurotransmitter. Probably has nitrosylase activity and mediates cysteine S-nitrosylation of cytoplasmic target proteins such SRR.
基因功能參考文獻：
1. Raynaud's phenomenon is associated with variation in gene NOS1 PMID: 29698501
2. A meta-analysis was performed for the association of rs2682826, rs3782206, rs499776, rs3782219, rs41279104, rs3782221, rs1879417, rs4767540, rs561712, and rs6490121 polymorphisms with schizophrenia. rs3782206 showed a strong association with schizophrenia in allelic, homozygote, dominant, and recessive models in Asians. In Caucasians, rs499776 was associated in homozygote, dominant, and recessive models. PMID: 28795310
3. Based on a quantitative comparison of their dendritic characteristics, the NOS1 neurons of humans and rats displayed a statistically significant difference PMID: 28720408
4. The neuronal NOS exon 7 in the experimental group has a higher ratio of G/T and T/T genotypes than the control group, and also that the neuronal NOS intron 4 in the experimental group has a significantly higher a/b genotype than the control group. This suggests that the neuronal NOS gene mutation could be related to the development of osteonecrosis of the femoral head. PMID: 28770971
5. High NOS1 expression is associated with Dilated Hearts. PMID: 27481317
6. NOS1-ex1f VNTR is associated with white matter microstructure in females with ADHD and healthy females. PMID: 28589541
7. selected nNOS polymorphisms do not significantly contribute to Parkinson's disease risk in north Indian population. PMID: 26081147
8. Lysophosphatidylcholine induced nNOS uncoupling and nNOS(Ser852) phosphorylation, reduced NO and H2O2 production and improved superoxide production by modulating ERK1/2 activity in endothelial cells. PMID: 28235709
9. NOS1 (missense variant rs79487279) was associated with bipolar disorder. PMID: 28195573
10. Data suggest that NOS1 and NOS2 play roles in stress-induced surge in nitric oxide (NO) production; NO serves as mediator for development of secondary neurological disorders associated with stress such as anxiety and anxiety disorders. [REVIEW] PMID: 28061969
11. nNOS mediates the human coronary vasodilator response to mental stress, predominantly through actions at the level of coronary resistance vessels. PMID: 28646032
12. These findings highlight a key role of the TLR4-NOS1-AP1 signaling axis in regulating macrophage polarization PMID: 28013342
13. Association between NOS1 and PTSD severity and stress was found. NOS1 was associated with resilience. PMID: 28465168
14. Data show that metastasis associated 1 (MTA1) represses neuronal nitric oxide synthase (nNOS) expression upon oxygen glucose deprivation (OGD)-induced oxidative stress. PMID: 27603575
15. Superoxide generation from NOS1 splice variants and its potential involvement in redox signal regulation has been described. PMID: 28126743
16. expression of neuronal NOS heterodimers in insect cells, where we take advantage of an exogenous heme-triggered chaperone-assisted assembly process, provides an approximately 43% yield in heterodimeric NOS. PMID: 27487179
17. NOS1 ex1f-VNTR is associated with anxiety-related traits. PMID: 26746182
18. The NOS1 exon18 variants might play a role in the risk of Parkinson's disease (Meta-Analysis) PMID: 27749554
19. nNOS gene variants contribute to OCD pathogenesis. PMID: 27739347
20. Findings demonstrate that the upregulation of cardiac NOS1 is not accompanied by an increase in NOS activity; it is partially translocated to the sarcolemma in ischemic hearts with direct relationship between its protein levels and systolic ventricular function. These results indicate that NOS1 may be significant in the pathophysiology of human ischemic heart disease. PMID: 27041589
21. The found of this study suggested that possible roles of NOS1 gene in working memory performance in ADHD patients PMID: 26233433
22. NOS1 rs7977109 and rs693534 genotypes and allelic variants are not associated with the risk for migraine in Caucasian Spanish people PMID: 26283425
23. These results of this study suggested that SNAP25 and NOS1 genotypes influence ADHD symptoms only in adults with ADHD. PMID: 26821215
24. CoCl2 increased nNOS expression and NO production in human neuroblastoma SK-N-SH cells by increasing HIF-1a expression and binding to a a hypoxia response element in the nNos promoter. PMID: 26458913
25. polymorphisms in the gene may be useful in identifying women at risk for osteoporosis. PMID: 25871004
26. DLC1 binding to nNOS-calmodulin complex does not affect the electron transport from the reductase to the oxygenase domain. PMID: 26923072
27. We conclude that DNM2 is a novel negative regulator of NO production in mouse collecting ducts. PMID: 26791826
28. analysis of the interaction between nNOS and dystrophin repeats 16 and 17 PMID: 26378238
29. the first structure of human neuronal nitric oxide syntahse PMID: 25286850
30. This study first-time showed highest Red Blood Cell (RBC)-Nitric Oxide Synthase activation and nitric oxide production in old RBC. PMID: 25902315
31. Results suggested a link between NOS1 gene polymorphism at rs3782206 and cognitive functions and their neural underpinnings at the right inferior frontal gyrus PMID: 25490993
32. Tetrahydrobiopterin:dihydrobiopterin ratio is lower in tumor tissues and, as a consequence, NOS activity generates more peroxynitrite and superoxide anion than nitric oxide, resulting in important tumor growth-promoting and antiapoptotic signaling properties. PMID: 25724429
33. Study found no significant differences either in the frequencies of the NOS1 rs7977109 and rs693534 genotypes or in the frequencies of the allelic variants of these SNPs in restless legs syndrome patients when compared with healthy controls PMID: 25300364
34. Studied and determined the localisation of neuronal NOS (nNOS) in human airway epithelium. PMID: 25460324
35. T allele of rs2293050 and A allele of rs2139733 in nNOS gene may contribute to increased susceptibility of LAA-caused ischemic stroke in Han Chinese PMID: 24668187
36. Results suggest that NOS1 SNPs interact with exposure to economic and psychosocial stressors to alter individual's susceptibility to depression PMID: 24917196
37. we discuss the idea that post-translational modifications of Ca2 + regulatory proteins via aberrant neuronal nitric oxide synthase (NOS1)-mediated nitroso-redox balance contribute to contractility defects in Heart failure PMID: 24801117
38. 2-methoxyestradiol specifically affects neuronal nitric oxide synthase and augments 3-nitrotyrosine level leading to osteosarcoma and immortalized hippocampal cell death. PMID: 25170949
39. Increased cellular expression of nNOS was accompanied by elevated NO generation in brains of heroin addicts and may have contributed to some of the known toxic effects of heroin. PMID: 23953641
40. Results identified NOS1 as a novel nitric oxide target in human skeletal muscle controlled by activity driven auto-nitrosylation mechanisms. PMID: 24251120
41. rADI not only reduced NO production but also caused cellular toxicity in nNOS-activated SH-SY5Y cells, suggesting a dual role for rADI in NOS-mediated neurotoxicity. PMID: 25126568
42. DNA methylation of NOS1 plays role in atherogenesis through regulation of NO production. PMID: 24622112
43. This review will focus on the role of Nitric Oxide Synthases (NOSes) the role they play in the pathogenesis of subarachnoid hemorrhage. PMID: 25366612
44. The NOS1 rs41279104 CT genotype was associated with good responders in postoperative ED patients. PMID: 24897285
45. Studied which NOS gene variants are most strongly associated with coronary heart disease (CHD) and hypertension and confirmed NOS1 as the most important NOS risk gene for CHD. PMID: 24713495
46. Population genetic variability in Sardinia (Italy) shows a positive and highly significant correlation between mortality determined by malaria infection and alleles (TGGA)7 of NOS2, (AAAAG)2 and (ATTT)10 of adNOS1, and (AAACA)11 of adNOS3 genes. PMID: 24573959
47. The resukts of this study demonistrated that the risk allele significantly decreases expression of NOS1 in the prefrontal cortex in patient with schizophrenia. PMID: 24220657
48. Genetic or pharmacologic inhibition of NOS1 reduced the growth of CXCL14-expressing fibroblasts. PMID: 24710408
49. The results showed that rs7308402 gene polymorphism of nNOS is related to ischemic stroke in Han Chinese of North China. PMID: 24082858
50. A report on the reduction of NOS1 expression in the anterior cingulate cortex in depressive patients. PMID: 22989585
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亞細胞定位：

Cell membrane, sarcolemma; Peripheral membrane protein. Cell projection, dendritic spine.
蛋白家族：

NOS family
組織特異性：

Isoform 1 is ubiquitously expressed: detected in skeletal muscle and brain, also in testis, lung and kidney, and at low levels in heart, adrenal gland and retina. Not detected in the platelets. Isoform 3 is expressed only in testis. Isoform 4 is detected
數據庫鏈接：

HGNC: 7872

OMIM: 163731

KEGG: hsa:4842

STRING: 9606.ENSP00000337459

UniGene: Hs.654410

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