MRAS Recombinant Monoclonal Antibody
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中文名稱：MRAS重組抗體
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貨號(hào)：CSB-RA014781MA1HU
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規(guī)格：￥1320
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圖片：
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IHC image of CSB-RA014781MA1HU diluted at 1:600 and staining in paraffin-embedded human prostate tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-Mouse IgG labeled by HRP and visualized using 0.05% DAB. -
Immunofluorescence staining of Hela cell with CSB-RA014781MA1HU at 1:200, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-Mouse IgG(H+L).
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其他：
產(chǎn)品詳情
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產(chǎn)品描述：CSB-RA014781MA1HU MRAS重組單克隆抗體是針對(duì)MRAS靶點(diǎn)研發(fā)的高特異性科研試劑，經(jīng)ELISA、免疫組化（IHC）和免疫熒光（IF）等多平臺(tái)嚴(yán)格驗(yàn)證，在IHC應(yīng)用中推薦1:20-1:200稀釋度，IF實(shí)驗(yàn)建議1:20-1:200稀釋比例。該抗體通過抗原親和力純化工藝制備，展現(xiàn)出優(yōu)異的靶標(biāo)結(jié)合特異性，在多種組織樣本中均能實(shí)現(xiàn)清晰的細(xì)胞膜/細(xì)胞質(zhì)定位信號(hào)，且背景干擾低，重復(fù)性穩(wěn)定。MRAS作為RAS癌基因家族成員，參與調(diào)控MAPK信號(hào)通路，在細(xì)胞增殖、分化及腫瘤發(fā)生發(fā)展中具有重要作用。本產(chǎn)品適用于探究MRAS在腫瘤微環(huán)境中的表達(dá)模式、癌癥相關(guān)信號(hào)通路調(diào)控機(jī)制以及細(xì)胞功能研究，可配合病理學(xué)分析、分子互作實(shí)驗(yàn)及細(xì)胞模型構(gòu)建，為腫瘤生物學(xué)基礎(chǔ)研究提供精準(zhǔn)的實(shí)驗(yàn)工具。
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Uniprot No.：
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基因名：
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別名：FLJ42964 antibody; M ras antibody; M-ras antibody; Mras antibody; Muscle and microspikes Ras antibody; Muscle RAS oncogene homolog antibody; Muscle Ras oncogene homologue antibody; Muscle Ras viral oncogene homolog antibody; R ras3 antibody; R-ras3 antibody; Ras related protein MRas antibody; Ras related protein RRas3 antibody; Ras-related protein M-Ras antibody; Ras-related protein R-Ras3 antibody; RASM_HUMAN antibody; Related Ras viral oncogene homolog 3 antibody; RRas3 antibody; XRas antibody
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反應(yīng)種屬：Human
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免疫原：Recombinant Human MRAS protein
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免疫原種屬：Homo sapiens (Human)
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標(biāo)記方式：Non-conjugated
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克隆類型：Monoclonal
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抗體亞型：Mouse IgG2a
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純化方式：Affinity-chromatography
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克隆號(hào)：17H12
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濃度：It differs from different batches. Please contact us to confirm it.
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保存緩沖液：Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4 -
產(chǎn)品提供形式：Liquid
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應(yīng)用范圍：ELISA, IHC, IF
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推薦稀釋比：
Application Recommended Dilution IHC 1:20-1:200 IF 1:20-1:200 -
Protocols：
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儲(chǔ)存條件：Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
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貨期：Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
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用途：For Research Use Only. Not for use in diagnostic or therapeutic procedures.
產(chǎn)品評(píng)價(jià)
相關(guān)產(chǎn)品
靶點(diǎn)詳情
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功能：Serves as an important signal transducer for a novel upstream stimuli in controlling cell proliferation. Activates the MAP kinase pathway.
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基因功能參考文獻(xiàn)：
- acute coronary syndrome in Czechs (30.4% vs 29.4% carriers of the minor T allele of MRAS[recessive model], p = 0.54; OR 1.05; 95% CI 0.89-1.24 for males and 32.1% vs 29.7% carriers of the minor T allele, p = 0.28; OR 1.12; 95% CI 0.91-1.37 for females) PMID: 29264877
- Results discovered for the first time that MRAS is recurrently mutated, indicating that MRAS mutations could drive tumorigenesis of Type IV gastric neoplasm. PMID: 27891760
- The association of the MARS rs6782181 polymorphism and serum lipid levels is different between the Mulao and Han populations, or between males and females in the both ethnic groups. PMID: 25973078
- The MRAS gene loci might have a minor effect in conferring susceptibility to coronary artery disease in the Chinese population. PMID: 25800439
- Both MRAS and SHOC2 play a key role in polarized migration. PMID: 24211266
- implicated in a novel pathway of neuronal differentiation by coupling specific trophic factors to the MAPK cascade through the activation of B-Raf PMID: 12138204
- These findings proved a crucial role of the cross-talk between two Ras-family GTPases M-Ras and Rap1, mediated by RA-GEF-2, in adhesion signaling. PMID: 17538012
- Identification of one new CAD risk locus on 3q22.3 in MRAS, and suggestive association with a locus on 12q24.31 near HNF1A-C12orf43. PMID: 19198612
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亞細(xì)胞定位：Cell membrane; Lipid-anchor; Cytoplasmic side.
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蛋白家族：Small GTPase superfamily, Ras family
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組織特異性：Expression highly restricted to the brain and heart.
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數(shù)據(jù)庫鏈接：
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