MICA Recombinant Monoclonal Antibody

說明書

中文名稱：

MICA重組抗體
貨號：

CSB-RA574653A0HU
規格：

￥1320
圖片：
IHC image of CSB-RA574653A0HU diluted at 1:80 and staining in paraffin-embedded human small intestine tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.49% DAB.

IHC image of CSB-RA574653A0HU diluted at 1:80 and staining in paraffin-embedded human liver cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.49% DAB.
其他：

產品詳情
產品評價
相關產品
靶點詳情

產品詳情

產品描述：

CSB-RA574653A0HU MICA重組單克隆抗體是經過嚴格驗證的科研用免疫檢測試劑，靶向MHC I類鏈相關蛋白A（MICA）。該蛋白作為細胞應激標志物，通過結合NK細胞和T細胞表面的NKG2D受體參與免疫監視機制，在腫瘤免疫逃逸、病毒感染應答及組織移植排斥等過程中發揮關鍵作用。本產品經ELISA和免疫組化（IHC）雙平臺驗證，在石蠟包埋組織切片中可清晰顯示MICA蛋白的定位表達，推薦使用稀釋比為1:50-1:200時呈現優異信號特異性。實驗數據顯示該抗體能有效識別天然構象及變性狀態下的靶蛋白，適用于腫瘤微環境分析、感染性疾病模型中的免疫調節研究以及自身免疫病理機制探索。批次間高度一致的結合活性保障了實驗數據的可重復性，為免疫相關疾病的基礎研究提供可靠工具，特別適用于病理樣本的免疫表型分析及體外蛋白互作研究。
Uniprot No.：

Q29983
基因名：

MICA
別名：

MHC class I polypeptide-related sequence A (MIC-A), MICA, PERB11.1
反應種屬：

Human
免疫原：

A synthesized peptide derived from Human MICA
免疫原種屬：

Homo sapiens (Human)
標記方式：

Non-conjugated
克隆類型：

Monoclonal
抗體亞型：

Rabbit IgG
純化方式：

Affinity-chromatography
克隆號：

9F1
濃度：

It differs from different batches. Please contact us to confirm it.
保存緩沖液：

Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.
產品提供形式：

Liquid
應用范圍：

ELISA, IHC

推薦稀釋比：

Application	Recommended Dilution
IHC	1:50-1:200

Protocols：

ELISA Protocol
Immunohistochemistry (IHC) Protocol
儲存條件：

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
貨期：

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
用途：

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產品評價

靶點詳情

功能：

Seems to have no role in antigen presentation. Acts as a stress-induced self-antigen that is recognized by gamma delta T-cells. Ligand for the KLRK1/NKG2D receptor. Binding to KLRK1 leads to cell lysis.
基因功能參考文獻：
1. Results provide further evidence that genetic variants of MICA influence soluble MICA levels, and that Val mismatch at position 129 increases cytomegalovirus infection and kidney acute rejection risk during the first year post-pancreas-kidney transplantation . PMID: 30181474
2. Role of MICA in different cancer types (review). PMID: 29635123
3. The MICA A5.1 polymorphism was associated with a better CT morphologic response to chemotherapy and a reduced risk of relapse after Colorectal Liver Metastases resection. PMID: 29969766
4. According to our analysis, three proteins, namely aristaless-like homeobox1 isoform X1 (ALX1), major histocompatibility complex polypeptide-related sequence A (MICA), and uncharacterized protein C14orf105 isoform X12 were found to be potential markers for Opisthorchis viverrini (OV)- infection, as they were predominantly found in all OV-infected groups PMID: 29936472
5. MICA-129 val/val genotype, associated with higher levels of circulating sMICA, may influence disease susceptibility and associate with increased severity of rheumatoid arthritis in south Indian Tamils. PMID: 28752674
6. rs12524487 in HLA-B/MICA was a genetic risk factor for Takayasu arteritis in a Chinese Han population and rs9366782 in this region was associated with ischemic brain disease in TA but not TA susceptibility. PMID: 28261975
7. Here we discovered that an HCMV protein named UL148A whose role was hitherto unknown is required for evasion of NK cells. We demonstrate that UL148A-deficient HCMV strains are impaired in their ability to downregulate MICA expression. PMID: 29950412
8. allele MICA*002:01/A9 and haplotype MICA*002:01-MICB*005:02 were negatively associated with respiratory syncytial virus respiratory tract infections. PMID: 28925058
9. Levels of serum MICA were highly correlated to liver disease severity in chronic hepatitis C patients who carried the MICA rs738409 A allele. PMID: 28427234
10. Strategies to block MICA-NKG2D interactions resulted in reductions in IFNgamma production. Depletion of monocytes in vivo resulted in decreased IFNgamma production by murine NK cells upon exposure to Ab-coated tumor cells PMID: 28724544
11. A significant association was found between the Val allele and Val/Val genotype and the risk of breast cancer in Tunisian women. PMID: 28742417
12. the present study revealed that DNA damagedependent MICA/B expression in insensitive cancer cells can be restored by chromatin relaxation via the HDAC/Suv39/G9a pathway. Collectively, manipulation of chromatin status by therapeutic cancer drugs may potentiate the antitumor effect by enhancing immune activation following radiotherapy and DNA damage-associated chemotherapy. PMID: 28677817
13. Findings show that the efficient expression of cell-surface major histocompatibility class I-related chain molecule A (MICA) in the osteosarcoma cells requires asparagine (Asn)-N-linked glycosylation of MICA. PMID: 29491059
14. Study identified the novel role of transcription factors GATA-2 and GATA-3 in suppressing MICA/B expression in HBV-infected human hepatoma cells. PMID: 27528231
15. new MICA allelic variant, MICA*007:07, was identified in an individual of Mongol ethnicity in the Inner Mongolia Autonomous Region, northern China PMID: 28371368
16. inhibition of BET proteins can enhance the expression of MICA, a ligand of the NKG2D receptor, in human MM cell lines and primary malignant plasma cells, rendering myeloma cells more efficient to activate NK cell degranulation PMID: 27903272
17. results indicate that the polymorphism of rs2256318 in MICA may contribute to the etiology of Preterm birth through interfering with placental development. These findings need to be further validated in larger and multi-ethnic populations. PMID: 28864994
18. The results of this study suggested that the MICB*009N allele might be a risk factor for SLE, whereas the MICB*014, MICA*010 and MICB*002 alleles were associated with reduced incidence of SLE in the study population. PMID: 29078849
19. New truncated MICA isoforms exhibit a range of functions that may drive unexpected immune mechanisms and provide new tools for immunotherapy PMID: 27342847
20. data reveal that MICA and PVR are directly regulated by human cytomegalovirus immediate early proteins, and this may be crucial for the onset of an early host antiviral response PMID: 27733551
21. This demonstrates for the first time that MICA/B is more broadly expressed in normal tissue and that expression is mainly intracellular with only a small fraction appearing on the cell surface of some epithelia and tumour cells. PMID: 28334733
22. selecting a MICA-matched donor significantly influences key clinical outcomes of HCT in which a marked reduction of GVHD is paramount. The tight linkage disequilibrium between MICA and HLA-B renders identifying a MICA-matched donor readily feasible in clinical practice. PMID: 27549307
23. MICA-129 matching is relevant in unrelated hematopoietic stem cell transplantation. PMID: 27811019
24. No evidence of an association between MICA*Del and nasopharyngeal carcinoma in the southern Chinese Han population. PMID: 27870115
25. These findings identify the major histocompatibility complex-related MICA as an immunogenetic factor that may functionally influence anti-BK polyomavirus immune responses and infection outcomes. PMID: 27130430
26. This study provided information on the distribution of MICA polymorphisms and linkage disequilibrium with HLA-B alleles in Brazilian renal-transplant candidates. A total of 19 MICA allele groups were identified. The most frequent allele groups were MICA*008 (21.6%), MICA*002 (17.0%) and MICA*004 (14.8%). The most common haplotypes were MICA*009-B*51 (7.8%), MICA*004-B*44 (6.06%) and MICA*002-B*35 (5.63%). PMID: 28419176
27. MICA*A4 protects against ulcerative colitis, whereas MICA*A5.1 is associated with abscess formation and age of onset. PMID: 26940143
28. MICA*012:05 differs from MICA*012:01 by a single synonymous C to T substitution at nucleotide position 269 in exon PMID: 27273902
29. there is no obvious correlation between the MMP9 -1562 C/T SNP and the concentrations of circulating MICA/B in the breast cancer patients. PMID: 27026046
30. Cirrhotic patients who carry MICA risk alleles and those without risk alleles but with high sMICA levels possessed the highest risk of HCC development once they failed antiviral therapy. PMID: 27998720
31. High levels of serum MICA is associated with acute myocardial infarction. PMID: 27306684
32. Findings shown here are important from an anthropologic perspective and will inform future studies of the potential role of MICA and MICB genes in allogeneic organ transplantation and HLA-linked disease association in populations of related ancestry. PMID: 27028549
33. MICA was significantly associated with the epithelial-to-mesenchymal transition gene set in clear cell renal cell carcinoma PMID: 26349747
34. Reversal of epigenetic silencing of MICA and MICB improves immune recognition and killing of Merkel cell carcinoma cells. PMID: 26902929
35. Our meta-analysis confirmed MICA-A6 could be responsible for BD in three ethnic regions and should probably be treated as a risk factor for Behcet's disease. PMID: 26875668
36. IFN-gamma rs2069727 and MICA rs2596542 polymorphisms may be related to the incidence of hepatocellular carcinoma. PMID: 26893439
37. gastric cancer patients who have tumors with high expression of MICA are more likely to benefit from adjuvant chemotherapy, immunotherapy and gastrectomy PMID: 26607264
38. Our results suggest that locally sustained expression of MICA and MICB in the tumor may contribute to the malignant progression of Gastric cancer(GC) and that expression of these ligands predicts an unfavorable prognosis in GC patients presenting large tumors. PMID: 26708143
39. In South Tunisian population, MICA plays a disease modifying role, rather than being an important gene in the susceptibility for developing of uveitis. PMID: 25468490
40. The reduced cell surface expression of NKG2D in response to engagement by MICA-129Met variants appeared to reduce the severity of acute graft-versus-host disease. PMID: 26483398
41. Estrogen upregulates MICA/B expression in human non-small cell lung cancer through the regulation of ADAM17. PMID: 25363527
42. This meta-analysis shows that the MICA-TM A6 allele and the MICA*009 allele are associated with BD susceptibility in various ethnic populations, and that MICA alleles are in strong linkage disequilibrium with HLA-B51 in BD. PMID: 26184953
43. The importance of the functional MICA-129 polymorphism in the severity of left ventricular ejection fraction in Chronic Chagas heart disease was addressed. PMID: 26129751
44. MICA polymorphisms do not appear to influence the development of ocular lesions in patients diagnosed with toxoplasmosis in this study population. PMID: 26672749
45. The study investigates the link between Behcet's Disease and two specific HLA alleles associated with Behcet's Disease (HLA-A*26:01 and HLA-B*51:01) in terms of their binding affinity to the MICA. PMID: 26331842
46. In conclusion DNT cell can significantly inhibit the growth of pancreatic carcinoma in vivo, and the mechanism may be involved in abnormal expressions of MICA and NKG2D. PMID: 26616050
47. Further studies of the association between HBV replication and MICA induction should be conducted. PMID: 26212443
48. In cervical adenocarcinoma (but not squamous cell carcinoma), low sMICA was positively related to recurrent disease, a higher FIGO stage and vaginal involvement. High sMICA levels were associated with better disease-free and disease-specific survival. PMID: 25871737
49. Serum MICA levels were related to tumor pathology, TNM stage, and kidney neoplasm metastasis. PMID: 26125933
50. High MICA expression is associated with Renal Cancer. PMID: 25987057
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相關疾病：

Psoriasis 1 (PSORS1); Psoriatic arthritis (PSORAS)
亞細胞定位：

Cell membrane; Single-pass type I membrane protein. Cytoplasm.
蛋白家族：

MHC class I family, MIC subfamily
組織特異性：

Widely expressed with the exception of the central nervous system where it is absent. Expressed predominantly in gastric epithelium and also in monocytes, keratinocytes, endothelial cells, fibroblasts and in the outer layer of Hassal's corpuscles within t
數據庫鏈接：

HGNC: 7090

OMIM: 177900

KEGG: hsa:100507436

UniGene: Hs.130838

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