MADCAM1 Recombinant Monoclonal Antibody
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中文名稱：MADCAM1重組抗體
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貨號：CSB-RA215817A0HU
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規(guī)格：￥1320
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圖片：
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Overlay Peak curve showing PC3 cells surface stained with CSB-RA215817A0HU (red line) at 1:100. The cells were incubated in 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*106cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated Goat Anti-rabbit IgG(H+L) at 1:200 dilution for 35min at 4℃.Control antibody (green line) was rabbit IgG (1ug/1*106cells) used under the same conditions. Acquisition of >10,000 events was performed.
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其他：
產(chǎn)品詳情
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產(chǎn)品描述：CSB-RA215817A0HU MADCAM1重組單克隆抗體是針對黏膜地址素細(xì)胞黏附分子1（MADCAM1）開發(fā)的高特異性科研試劑。MADCAM1是一種主要表達(dá)于血管內(nèi)皮細(xì)胞的跨膜蛋白，尤其在腸道相關(guān)淋巴組織中高表達(dá)，通過結(jié)合α4β7整合素和L-選擇素介導(dǎo)淋巴細(xì)胞向黏膜組織的定向遷移，在免疫監(jiān)視和炎癥反應(yīng)中發(fā)揮關(guān)鍵調(diào)控作用。本產(chǎn)品經(jīng)ELISA和流式細(xì)胞術(shù)（FC）嚴(yán)格驗證，可實現(xiàn)對天然及重組MADCAM1蛋白的特異性識別，其中流式檢測推薦使用稀釋比例為1:50-1:200。該抗體適用于體外研究MADCAM1在免疫細(xì)胞歸巢、黏膜屏障免疫調(diào)節(jié)及慢性炎癥疾病機制中的功能，例如通過流式細(xì)胞術(shù)分析內(nèi)皮細(xì)胞表面MADCAM1的表達(dá)動態(tài)，或結(jié)合體外黏附實驗探究淋巴細(xì)胞與血管內(nèi)皮相互作用的分子基礎(chǔ)。作為重組單克隆抗體，其具有批次穩(wěn)定性和高親和力優(yōu)勢，可為免疫學(xué)、炎癥相關(guān)疾病研究提供可靠工具。
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Uniprot No.：
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基因名：
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別名：Mucosal addressin cell adhesion molecule 1 (MAdCAM-1) (hMAdCAM-1), MADCAM1
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反應(yīng)種屬：Human
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免疫原：A synthesized peptide derived from human MADCAM1
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免疫原種屬：Homo sapiens (Human)
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標(biāo)記方式：Non-conjugated
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克隆類型：Monoclonal
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抗體亞型：Rabbit IgG
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純化方式：Affinity-chromatography
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克隆號：12B5
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濃度：It differs from different batches. Please contact us to confirm it.
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保存緩沖液：Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.
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產(chǎn)品提供形式：Liquid
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應(yīng)用范圍：ELISA, FC
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推薦稀釋比：
Application Recommended Dilution FC 1:50-1:200 -
Protocols：
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儲存條件：Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
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貨期：Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
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用途：For Research Use Only. Not for use in diagnostic or therapeutic procedures.
產(chǎn)品評價
相關(guān)產(chǎn)品
靶點詳情
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功能：Cell adhesion leukocyte receptor expressed by mucosal venules, helps to direct lymphocyte traffic into mucosal tissues including the Peyer patches and the intestinal lamina propria. It can bind both integrin alpha-4/beta-7 and L-selectin, regulating both the passage and retention of leukocytes. Isoform 2, lacking the mucin-like domain, may be specialized in supporting integrin alpha-4/beta-7-dependent adhesion strengthening, independent of L-selectin binding.
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基因功能參考文獻(xiàn)：
- Both the ACT-1 assay and the MAdCAM-1 assay demonstrated acceptable reproducibility and repeatability. The assays were sufficiently stable to allow for clinical use. During clinical testing the assays demonstrated that vedolizumab was able to saturate peripheral cells at all doses tested. PMID: 25908521
- Doxo-induced apoptosis has a role in inhibiting hepatocellular carcinoma by targeting Madcam1 and AKT and blocking protein translation initiation PMID: 26124182
- MADCAM1 is up-regulated in liver cirrhosis with expression on peribiliary vascular plexus and lymphoid aggregates PMID: 23839340
- Domain 1 of mucosal addressin cell adhesion molecule has an I1-set fold and a flexible integrin-binding loop. PMID: 23297416
- Findings suggest that the inactive alpha4beta7 and alpha4beta7 activated by different stimuli have distinct conformations with different structural requirements for MAdCAM-1 binding. PMID: 21296888
- Ca2+ is essential for cell tethering in flow and rolling interactions through alpha 4 integrins with MAdCAM-1 as substrate; Mg2+ promotes firm adhesion of cells on MAdCAM-1 but with much lower tethering efficiency in shear flow. PMID: 11705388
- A crystal structure for the two extracellular amino-terminal domains of human MAdCAM-1 PMID: 11807247
- more extensive expression of MAdCAM-1 in Crohn's disease (CrD), than in ulcerative colitis, which could contribute not only to mucosal inflammation, but also to transmural inflammation in CrD. PMID: 12100519
- MAdCAM-1/integrin alpha4beta7 homing system may participate in gastric inflammation in response to H pylori-infection and contributes to MALT formation, typically leading to the development of nodular gastritis. PMID: 14669317
- a marker of graft rejection in small intestine transplantation PMID: 15050155
- PI3-K/Akt is involved in the gut-specific differentiation of human intestinal microvascular endothelial cells, which results in expression of the mucosal addressin MAdCAM-1 PMID: 15483224
- Immobilized CCL25 and CCL28 are both able to trigger integrin alpha(4)beta(7)-dependent lymphocyte arrest on MAdCAM-1 under shear flow. PMID: 18308860
- mucosal addressin cell adhesion molecule 1 may represent an important determinant for the generation of mucosal damage in celiac disease PMID: 19157500
- MADCAM1 gene polymorphisms may be associated with the risk of chronic GVHD and may, also, affect mortality related to aHSCT. PMID: 19286444
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亞細(xì)胞定位：Membrane; Single-pass type I membrane protein.
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組織特異性：Highly expressed on high endothelial venules (HEV) and lamina propia venules found in the small intestine, and to a lesser extent in the colon and spleen. Very low levels of expression found in pancreas and brain. Not expressed in the thymus, prostate, ov
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