GDF15 Recombinant Monoclonal Antibody

說明書

中文名稱：

GDF15重組抗體
貨號：

CSB-RA859530MA1HU
規格：

￥1320
圖片：
IHC image of CSB-RA859530MA1HU diluted at 1：100 and staining in paraffin-embedded human prostate cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-human polymer IgG labeled by HRP and visualized using 0.05% DAB.
其他：

產品詳情
產品評價
相關產品
靶點詳情

產品詳情

產品描述：

GDF15重組單克隆抗體（CSB-RA859530MA1HU）是針對生長分化因子15（GDF15）研發的高特異性科研工具。GDF15作為TGF-β超家族成員，在細胞應激響應、能量代謝調控及炎癥反應中發揮重要作用，其異常表達與代謝綜合征、腫瘤進展及心血管疾病密切相關。本產品經ELISA和免疫組化（IHC）雙重驗證，在IHC實驗中推薦使用1:50-1:200稀釋比例，適用于石蠟包埋組織切片的精準定位分析。通過嚴格的生產質控體系保障抗體具有優異的批間一致性，可特異性識別天然與重組GDF15蛋白，有效避免非特異性交叉反應。該抗體為研究GDF15在代謝調控通路、腫瘤微環境調控及心血管病理機制中的分子作用提供可靠支持，適用于細胞培養上清或生物體液樣本中GDF15的體外定量檢測，以及疾病模型組織的蛋白表達譜研究，為探索相關疾病的分子機制及潛在干預靶點提供重要技術支撐。
Uniprot No.：

Q99988
基因名：

GDF15
別名：

GDF15 Recombinant Monoclonal Antibody
反應種屬：

Human
免疫原：

Recombinant Human GDF15 protein
免疫原種屬：

Homo sapiens (Human)
標記方式：

Non-conjugated
克隆類型：

Monoclonal
抗體亞型：

hIgG1
純化方式：

Affinity-chromatography
克隆號：

5D2
濃度：

It differs from different batches. Please contact us to confirm it.
保存緩沖液：

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
產品提供形式：

Liquid
應用范圍：

ELISA, IHC

推薦稀釋比：

Application	Recommended Dilution
IHC	1:50-1:200

Protocols：

ELISA Protocol
Immunohistochemistry (IHC) Protocol
儲存條件：

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
貨期：

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
用途：

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產品評價

靶點詳情

功能：

Regulates food intake, energy expenditure and body weight in response to metabolic and toxin-induced stresses. Binds to its receptor, GFRAL, and activates GFRAL-expressing neurons localized in the area postrema and nucleus tractus solitarius of the brainstem. It then triggers the activation of neurons localized within the parabrachial nucleus and central amygdala, which constitutes part of the 'emergency circuit' that shapes feeding responses to stressful conditions. On hepatocytes, inhibits growth hormone signaling.
基因功能參考文獻：
1. Data indicate that LINC01133 inhibited OSCC metastasis via a feedback regulation loop of reciprocal inhibition with GDF15. PMID: 30332510
2. Our results provide valuable evidence that GDF15 is related with first-line chemo-resistance, with highly expressed GDF15 being a strong and an independent indicator of shorter PFS in EOC patients. PMID: 29580231
3. Placenta and appetite genes GDF15 and IGFBP7 are associated with hyperemesis gravidarum. PMID: 29563502
4. These results suggest that upregulation of NAG-1 contributes to trichostatin A-induced apoptosis in human endometriotic stromal cells. PMID: 29157123
5. GDF-15 is a promising biomarker for prediction of HF and death due to CHD in the general population, which may provide prognostic information to already established clinical biomarkers. PMID: 29771963
6. the association between GDF-15 and contrast-induced nephropathy in acute myocardial infarction patients after percutaneous coronary intervention PMID: 29791474
7. Age-dependent GDF-15 centile values developed from a population of apparently healthy older adults are independently predictive of all-cause mortality PMID: 29714603
8. To the best of our knowledge, this is the first documentation of cytokine GDF-15 presence in follicular fluid. Its concentration was not associated with donor/patient fertility status. Our data also show that GDF-15 is expressed and inducible in both normal healthy and cancerous granulosa cells, as well as in oocytes PMID: 29948426
9. Findings indicate that global platelet function was positively correlated with serum GDF-15 concentration. PMID: 28597461
10. GDF-15 in blood is more accurate in differentiating pancreatic mass due to chronic pancreatitis from pancreatic adenocarcinoma than Ca19-9. the combination of GDF-15 with Ca125 is remarkably useful in differentiating pancreatic mass. PMID: 29171983
11. Our results show GDF-15 as an effective biomarker in patients with metastatic colorectal cancer PMID: 29400662
12. This study found that the mean serum GDF15 levels in the Parkinson Disease patients were significantly higher than those of the healthy control group. Secondly, in the Parkinson Disease patients, the GDF15 levels were significantly higher in the men than in the women. PMID: 28787735
13. Elevated GDF-15 correlates with lack of reverse remodeling and increased mortality after TAVR and improves risk prediction of mortality. PMID: 29222133
14. Initial serum GDF15 level showed an inverse correlation with serum glomerular filtration rate and was associated with worse renal outcome in immunoglobulin A nephropathy. PMID: 28869715
15. data reveal that YAP promotes metastasis of breast cancer cells by repressing GDF15 transcription and present a novel molecular mechanism underlying the pro-metastasis function of YAP oncoprotein, with the implication of a therapeutic avenue for breast cancer treatment. PMID: 29499325
16. GDF15 was significantly correlated with mitochondrial disease severity and the proportion of ragged-red fibers identified in skeletal muscle. PMID: 27889897
17. GDF-15 serum levels were elevated in patients with Systemic sclerosis and correlated with the extent of skin fibrosis, and it was found to be higher in Systemic sclerosis patients with interstitial pulmonary fibrosis PMID: 28872977
18. Enhanced radiosensitivity and decreased migration in cells caused by CDP138 knockdown are partially dependent on GDF15 inhibition. PMID: 28880265
19. these results suggest that increased GDF-15-as observed in lungs of smokers and patients with COPD-contributes to cigarette smoke-induced pulmonary inflammation. PMID: 28145442
20. High GDF15 levels are associated with increased risk of major amputation and/or death in peripheral arterial disease patients. PMID: 28855167
21. we found that GDF-15 -3148C/G polymorphism (CC genotype) might have a correlation with acute ST-elevation myocardial infarction occurrence and the formation of collateral circulation. PMID: 29489980
22. Data show that serum growth differentiation factor 15 (GDF15)levels were significantly higher in subjects with thyroid nodules compared with nodule negative subjects, and this was influenced by age. PMID: 28489602
23. Findings suggest that GDF15 induces tumor sphere formation through GDF15-ERK1/2-GDF15 circuits, leading to maintenance of GDF15high cancer stem-like cells. PMID: 28206960
24. These authors demonstrate that blocking cardiomyocyte production of GDF15 normalizes circulating GDF15 level and restores liver growth hormone signaling, establishing GDF15 as a bona fide heart-derived hormone that regulates pediatric body growth. PMID: 28572090
25. Authors demonstrated that GDF15 contributed to radioresistance of HNC, as determined by both gain- and lost-of-functional experiments. Authors further showed that GDF15 facilitated the conversion of cancer stemness, as assessed by the promotion of CD44+ and ALDH1+ cell populations and spheroid cell formation. PMID: 27903972
26. this study, for the first time, demonstrates that GDF15 is a promising target for preventing cold I/R injury in heart transplantation. This study also shows that the resultant protective effects are mediated by the Foxo3 and NFkappaB signaling pathways PMID: 28388574
27. High circulating GDF-15 levels are predictive of secondary cardiovascular events in women but not in men with carotid atherosclerosis. PMID: 28389123
28. In patients with chronic obstructive pulmonary disease, high levels of GDF15 were independently associated with a higher yearly rate of exacerbations, higher mortality and increased decline in both FEV1 and FVC. PMID: 28298399
29. Data suggested NAG-1 protein as a crucial mediator of epithelial ovarian cancer (EOC) progression and resistance to the standard first-line chemotherapy against EOC, particularly in MyD88-positive ovarian cancer stem-like cells. PMID: 27708225
30. The GDF-15 gene rs1804826G/T polymorphism and sGDF-15 levels are associated with ischemic stroke in the Chinese population. PMID: 28526273
31. NSAID-activated gene 1 (NAG-1) has been assessed for its involvement in cellular integrity and pathogenesis in mucosal inflammation and carcinogenesis. [review] PMID: 28455268
32. showed association between high level of GDF-15, determined at the first 24 hours after acute coronary syndrome, and chronic heart failure progression after 12 months. PMID: 29099703
33. Results provide evidence that GDF15 contributes to the cancer-associated fibroblasts-mediated chemoprotection of AML cells. PMID: 27643489
34. serum GDF15 was not altered in the first trimester in women destined to develop late-onset preeclampsia. PMID: 27173615
35. even very small amounts of contaminating TGF-b in purified protein batches may influence the experimental results given that receptors for TGF-b are present. When we attempted to characterize possible receptors for the TGF-b superfamily ligand GDF15, striking similarities between GDF15-induced activities and known TGF-b activities were found. PMID: 29161287
36. In ever-smokers with COPD free of clinical CVD, GDF-15 contributes independently to subclinical coronary atherosclerosis. TRIAL REGISTRATION: ClinicalTrials.gov, NCT00608764 . Registered 28 January 2008. PMID: 28245821
37. This is the first study showing that adolescent male swimmers have higher PTX3 levels than sedentary controls and that there is a linear relationship between PTX3 and GDF-15. PMID: 28664745
38. GDF-15 at 1 month after an acute coronary syndrome is related to the risk of bleeding during dual antiplatelet therapy and provides additional information on the bleeding risk beyond baseline GDF-15 levels. PMID: 28411246
39. NF-kappaB/GDF-15 regulatory axis is important for tumor cells in evading macrophage immune surveillance during the early stages of tumorigenesis PMID: 28891811
40. Our data indicated that GDF-15 plays an important role in iron metabolism in SAA. GDF-15 might be a novel target for SAA therapy. PMID: 28385068
41. These results suggest that rs1054564 confers allele-specific translational repression of GDF15 via hsa-miR-1233-3p. PMID: 28806401
42. Serum MIC-1 levels in PUL were not able to categorically diagnose EP, however, MIC-1 could distinguish women with an EP that required medical intervention and those women whose PULs spontaneously resolved PMID: 23824055
43. HIF-1alpha and GDF15 expression are inversely related under normoxia and hyperoxia. PMID: 28734801
44. PPIX concentrations correlated with both growth differentiation factor (GDF) 15 (p = 0.012) and male gender (p = 0.015). PMID: 28185024
45. Data show that growth differentiation factor 15 (GDF15) knockdown significantly inhibited the growth and metastasis of cancer stem-like sphere cells (SCs) through AKT/GSK-3beta/beta-catenin pathway suppression. PMID: 28199981
46. epithelial restitution was promoted by enhanced NAG-1 expression and subsequent enterocyte locomotion during the early wound-healing process PMID: 27421482
47. serum GDF15 may be a useful diagnostic and prognostic biomarker for colorectal cancer. PMID: 26990020
48. These findings highlight an important contribution of GDF15 in promoting airway epithelial senescence upon cigarette smoke exposure. PMID: 27093475
49. The results of this study, including a robust induction of GDF-15 expression by several external factors known to elevate IOP, and rGDF-15-induced increase in contractility, cell adhesion, and the levels of ECM proteins and alphaSMA in TM cells PMID: 27918822
50. GDF-15 captures distinct aspects of cardiovascular disease development, progression, and prognosis, which are not represented by clinical risk predictors and other biomarkers. The usefulness of GDF-15 to guide management decisions and discover new treatment targets should be further explored. PMID: 28062617
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亞細胞定位：

Secreted.
蛋白家族：

TGF-beta family
組織特異性：

Highly expressed in placenta, with lower levels in prostate and colon and some expression in kidney. Detected in plasma (at protein level).
數據庫鏈接：

HGNC: 30142

OMIM: 605312

KEGG: hsa:9518

STRING: 9606.ENSP00000252809

UniGene: Hs.616962

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