DEFB1 Recombinant Monoclonal Antibody

說明書

中文名稱：

DEFB1重組抗體
貨號：

CSB-RA153094A0HU
規格：

￥1320
圖片：
Overlay Peak curve showing HepG2 cells surface stained with CSB-RA153094A0HU (red line) at 1:50. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1μg/1*10⁶cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated Goat Anti-rabbit IgG(H+L) at 1:200 dilution for 35min at 4℃.Control antibody (green line) was rabbit IgG (1μg/1*10⁶cells) used under the same conditions. Acquisition of >10,000 events was performed.
其他：

產品詳情
產品評價
相關產品
靶點詳情

產品詳情

產品描述：

CSB-RA153094A0HU DEFB1重組單克隆抗體是一款經ELISA和流式細胞分析（FC）驗證的高特異性科研用抗體，靶向人β-防御素1（DEFB1）。DEFB1作為先天免疫系統的關鍵效應分子，在宿主防御中通過直接抗菌活性和免疫調節功能參與抗感染及炎癥反應調控。本抗體在流式細胞實驗中推薦使用稀釋比例為1:50至1:200，可精準識別內源性DEFB1蛋白，適用于檢測多種細胞模型中該蛋白的表達分布及動態變化。驗證數據表明其具有優異的抗原結合能力和低交叉反應性，適用于探究DEFB1在感染性疾病、炎癥調控或上皮屏障功能等相關領域的分子機制。該產品采用重組單克隆抗體技術生產，具備高批次間一致性，可為免疫細胞功能研究、病原體-宿主相互作用分析及免疫相關信號通路探索提供可靠工具，嚴格限于科學研究使用。
Uniprot No.：

P60022
基因名：

DEFB1
別名：

Beta-defensin 1 (BD-1) (hBD-1) (Defensin, beta 1), DEFB1, BD1 HBD1
反應種屬：

Human
免疫原：

A synthesized peptide derived from Human DEFB1
免疫原種屬：

Homo sapiens (Human)
標記方式：

Non-conjugated
克隆類型：

Monoclonal
抗體亞型：

Rabbit IgG
純化方式：

Affinity-chromatography
克隆號：

1F5
濃度：

It differs from different batches. Please contact us to confirm it.
保存緩沖液：

Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.
產品提供形式：

Liquid
應用范圍：

ELISA, FC

推薦稀釋比：

Application	Recommended Dilution
FC	1:50-1:200

Protocols：

ELISA Protocol
Flow cytometry (FC) Protocol
儲存條件：

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
貨期：

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
用途：

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產品評價

靶點詳情

功能：

Has bactericidal activity. May act as a ligand for C-C chemokine receptor CCR6. Positively regulates the sperm motility and bactericidal activity in a CCR6-dependent manner. Binds to CCR6 and triggers Ca2+ mobilization in the sperm which is important for its motility.
基因功能參考文獻：
1. The allelic distribution of the polymorphism rs12355840 in miRNA202 was associated with caries experience in the Brazilian Manaus group. In the Ribeirao Preto group, the allelic and genotypic distributions in the polymorphism rs11362 in DEFB1 were associated with caries experience. PMID: 29727722
2. These studies identified SNPs that were cis-eQTLs for DEFB1 and AHR and, which were associated with variation in plasma KYN concentrations that were related to severity of major depressive disordersymptoms. PMID: 29317604
3. a considerable connection of DEFB1 and TCF7L2 gene polymorphisms with nephrolithiasis PMID: 29959006
4. identification of critical promoter regions of DEFB1 that are epigenetically influenced by lactobacilli in vaginal keratinocytes PMID: 28508168
5. Endometrial tissues and epithelial cells constitutively secreted both BD-1 and -2 mostly at the apical compartment. Both genistein and daidzein induced BDs secretion which reached a peak at 5-15 min. The apical secretion of BDs was coincidence with increased Cl- secretion induced by genistein. PMID: 29905453
6. The polymorphisms in DEFB1 were not associated with hBD salivary level and caries experience (p > 0.05) in children. PMID: 28343232
7. These findings suggest a role for hBD-1 in psoriasis pathogenesis PMID: 28067065
8. Significant associations were found between periodontitis and g. -20G> A (rs11362) and g. -44C> G (rs1800972) SNPs in DEFB1 gene as well as p.Ala29Thr (rs1126477) and p.Lys47Arg (rs1126478) SNPs in LTF gene. PMID: 28485077
9. hBD-1 potentiates the induction of in vitro osteoclastogenesis by RANKL via enhanced phosphorylation of the p44/42 MAPKs. PMID: 28709835
10. findings lead us to hypothesize an involvement of hBD-1 mediated innate immunity in the modulation of the susceptibility towards adeno-tonsillar hypertrophy development. PMID: 29501294
11. Two DEFB1 SNPs were significantly associated with the DMFT(decayed, missing, filled teeth) index. PMID: 27846636
12. Multivariate logistic regression analysis showed that hBD-1 basal cell loss (>/=20% of prostatic glands in total cores) is an independent factor for predicting prostatic adenocarcinoma (odds ratio: 4.739, confidence interval: 1.093-20.554, p = 0.038). hBD-1 loss of basal cells is a useful indicator to identify extremely high-risk patients with initially negative biopsy. PMID: 28885732
13. It was concluded that bile acids can differentially regulate colonic epithelial beta-defensin expression and secretion; the authors discuss the implications for intestinal health and disease.- PMID: 28487283
14. data provide important insight into the in vivo kinetics of HBD expression, the mechanism of HBD1 induction by HIV-1, and the role of HBDs in the early innate response to HIV-1 during acute infection PMID: 28253319
15. CpG methylation frequencies in the DEFB1 low CpG-content promoter were significantly higher in the prostate malignant tissues than in adjacent benign tissues. PMID: 27835705
16. DEFB1 - genotypes were correlated with the hBD-1 salivary concentration. PMID: 27770642
17. Coding missense and nonsense single nucleotide polymorphisms result in less active HBD1 antibacterial activity against Escherichia coli. PMID: 27160989
18. A significant difference was found between the levels of staining of TLR-2, TLR-4 and hBD-1 in different lesions from the epidermis, inflammation region, dermis and skin appendages (p < 0.05) in patients with acne vulgaris. PMID: 26695933
19. Expression of hBD-1, hBD-2, hBD-3, and hBD-4 in healthy and chronic periodontitis gingiva. PMID: 26874342
20. The -44 CC genotype of the beta-defensin-1 gene (DEFB1 rs1800972) may be associated with susceptibility to chronic periodontitis in Japanese. PMID: 24276427
21. No clear association was found between the DEFB1 polymorphisms and tuberculosis. PMID: 26991287
22. DEFB1 polymorphisms alone may not influence pathology susceptibility, however they could possibly concur, together with other factors involved in the genetic control of innate immune system, in the predisposition towards recurrent tonsillitis PMID: 26968045
23. beta-defensin-1 production is dysregulated in the epithelium of patients with chronic obstructive pulmonary disease PMID: 26568662
24. dental plaque amount, lactobacilli count, age, and saliva buffer capacity, as well as DEFB1 gene polymorphism, explained a total of 87.8% of variations in DMFT scores. PMID: 26377569
25. Our meta-analysis provides evidence that DEFB1 genetic polymorphisms rs11362G>A, rs1800972C>G and rs1799946G>A are important contributing factors to the development of digestive diseases PMID: 26232989
26. Association of infectious pathology of upper respiratory tract and development of periodontitis diseases with markers in DEFB1 (-44G/C) and TLR2 Arg753Gln and Arg677Trp genes was determined. PMID: 27029120
27. Genotypes and alleles of the DEFB1 gene found in long-living individuals can be considered as the factors that increase the probability of longevity and favorable course of age-related diseases. PMID: 26028230
28. there is a significant link between innate immunity deregulation through disruption of cationic peptides (hBDs) and the potential development of colon cancer. PMID: 26038828
29. Possible association between DEFB1 polymorphisms and in vivo salivary levels of hBD-1 in an Italian population of 40 healthy individuals, finding a genotype-related different expression with two of these polymorphisms: -52G > A and -44C > G. PMID: 25939140
30. We found that RNA interference-induced Caspase-12 silencing increased NOD1, hBD1 and hBD2 expression PMID: 25503380
31. A-dependent DEFB1 upregulation below 20 mM predicts in vitro antimicrobial activity as well as glucose- and AA-dependent CAMP and IFNG upregulation. PMID: 25815330
32. We conclude that plant-made recombinant beta-defensins (phBD-1 and phBD-2) are promising antimicrobial substances and have the potential to become additional tools against salmonellosis, particularly when used in combination. PMID: 25417183
33. The DEFB1 c.-44C>G polymorphism of the GG protective genotype was much less frequent among multiple sclerosis patients than among the controls. PMID: 26434201
34. beta defensin 1 gene -20G/A, -44C/G and -52G/A single nucleotide polymorphisms were not associated with chronic tonsillitis. PMID: 25683590
35. Our results suggest the involvement of DEFB1 polymorphisms, in particular that of the c.5*G>A SNP, in the susceptibility of women from Northeast Brazil to HPV infection. PMID: 25410996
36. Functional polymorphisms in IL1B and DEFB1 influence susceptibility to mold infection in solid-organ transplant recipients. PMID: 25398456
37. DEFB1 minor haplotypes were also associated with an increased risk of developing Atypical Squamous Cells of Undetermined Significance lesions, being significantly more frequent in HPV negative women with lesions, than without lesions. PMID: 24435641
38. The present finding explains a common defect in male infertility associated with both asthenozoospermia and leukocytospermia, indicating a dual role of DEFB1 in defending male fertility. PMID: 25122636
39. GG genotype of C-44G SNP in DEFB1 gene may result in decreased defensin beta-1 production in diabetes. PMID: 25083086
40. These findings associate DEFB1 5'UTR polymorphisms with HIV-1/AIDS in Mexican women for the first time. PMID: 25001249
41. hBD-1 suppresses tumor migration and invasion of oral squamous cell carcinoma. PMID: 24658581
42. Data indicate that CCL2 and IL-10 released from burn patient lineage-CD34+CD31- cells were shown to be responsible for their inhibitory activities on the production of beta-defensin 1 (HBD-1) by epidermal keratinocytes. PMID: 24498256
43. A possible involvement of DEFB1 gene in inflammatory bowel diseases. PMID: 24034632
44. genetic variant rs11362 in DEFB 1 influences caries susceptibility in CL/P children. The results support the hypothesis that expression of DEFB 1 in saliva may serve as a biomarker for future caries risk PMID: 23964634
45. This gene encodes a peptide with antimicrobial activity against S. aureus and H. influenzae. PMID: 20100591
46. HBD-1 serum levels in chronic kidney disease patients are inversely related to estimated glomerular filtration rate. PMID: 24356550
47. Our findings suggest interplay between hBD-1 and neuroimmunological responses in Alzheimer's disease PMID: 24139179
48. IRF5, but not TLR4, DEFEB1, or VDR, is associated with the risk of ulcerative colitis in a Han Chinese population. PMID: 23971939
49. Data suggest that levels of DEFB1 and DEFB2 are highest in colostrum and decrease in human milk over course of lactation; abundance of DEFB1 in colostrum may have protective against upper respiratory infection in infants up to 6 months. PMID: 24124699
50. The salivary beta-defensin concentration was significantly higher in patients with oral mucosal diseases than in healthy volunteers; furthermore, in patients, the concentration was significantly higher before treatment than after treatment. PMID: 24564045
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亞細胞定位：

Secreted. Membrane.
蛋白家族：

Beta-defensin family
組織特異性：

Blood plasma. Sperm. Highly expressed in the lower head and midpiece of sperm. Significantly reduced levels found in the sperms of asthenozoospermia and leukocytospermia patients (at protein level).
數據庫鏈接：

HGNC: 2766

OMIM: 602056

KEGG: hsa:1672

STRING: 9606.ENSP00000297439

UniGene: Hs.32949

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