CXCR4 Recombinant Monoclonal Antibody

說明書

中文名稱：

CXCR4重組抗體
貨號：

CSB-RA006254MA01HU
規格：

￥1320
圖片：
The Binding Activity of Human CXCR4 with Anti-CXCR4 recombinant Antibody
Activity: Measured by its binding ability in a functional ELISA. Immobilized Human CXCR4 (CSB-MP006254HU(F1)) at 10 μg/mL can bind Anti-CXCR4 recombinant antibody, the EC₅₀ is 101.7-253.6 ng/mL.
其他：

產品詳情
產品評價
相關產品
靶點詳情

產品詳情

產品描述：

CSB-RA006254MA01HU CXCR4重組單克隆抗體是針對人源CXCR4靶點開發的高特異性科研試劑。CXCR4作為趨化因子受體家族成員，在細胞遷移、免疫調節及腫瘤轉移等病理生理過程中發揮關鍵作用，并與HIV病毒入侵宿主細胞機制密切相關。本產品采用重組表達技術制備，具有高批次一致性和低交叉反應性，經嚴格驗證可特異性識別天然構象的CXCR4蛋白。在ELISA實驗中展現出優異的檢測靈敏度和重復性，適用于體外分析CXCR4蛋白表達水平及受體-配體相互作用研究。該抗體可支持腫瘤微環境研究、免疫細胞趨化性實驗、HIV感染機制探索等科研場景，特別適用于靶向CXCR4信號通路的藥物篩選或細胞遷移功能分析。通過嚴格的質控體系確保抗體性能穩定，為研究者提供可靠工具，但需注意本產品僅限于科學研究使用。
Uniprot No.：

P61073
基因名：

CXCR4
別名：

CXCR4; C-X-C chemokine receptor type 4; CXC-R4; CXCR-4; FB22; Fusin; HM89; LCR1; Leukocyte-derived seven transmembrane domain receptor; LESTR; Lipopolysaccharide-associated protein 3; LAP-3; LPS-associated protein 3; NPYRL; Stromal cell-derived factor 1 receptor; SDF-1 receptor; CD antigen CD184
反應種屬：

Human
免疫原：

Recombinant Human CXCR4 protein
免疫原種屬：

Homo sapiens (Human)
標記方式：

Non-conjugated
克隆類型：

Monoclonal
抗體亞型：

hIgG4(S228P)
純化方式：

Affinity-chromatography
克隆號：

2D4
濃度：

It differs from different batches. Please contact us to confirm it.
保存緩沖液：

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
產品提供形式：

Liquid
應用范圍：

ELISA
Protocols：

ELISA Protocol
儲存條件：

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
貨期：

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
用途：

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產品評價

靶點詳情

功能：

Receptor for the C-X-C chemokine CXCL12/SDF-1 that transduces a signal by increasing intracellular calcium ion levels and enhancing MAPK1/MAPK3 activation. Involved in the AKT signaling cascade. Plays a role in regulation of cell migration, e.g. during wound healing. Acts as a receptor for extracellular ubiquitin; leading to enhanced intracellular calcium ions and reduced cellular cAMP levels. Binds bacterial lipopolysaccharide (LPS) et mediates LPS-induced inflammatory response, including TNF secretion by monocytes. Involved in hematopoiesis and in cardiac ventricular septum formation. Also plays an essential role in vascularization of the gastrointestinal tract, probably by regulating vascular branching and/or remodeling processes in endothelial cells. Involved in cerebellar development. In the CNS, could mediate hippocampal-neuron survival.; (Microbial infection) Acts as a coreceptor (CD4 being the primary receptor) for human immunodeficiency virus-1/HIV-1 X4 isolates and as a primary receptor for some HIV-2 isolates. Promotes Env-mediated fusion of the virus.
基因功能參考文獻：
1. Functional analysis in human breast cancer cells showed that LL-37 induced the internalization of CXCR4 through approaching Glu268, the residue of CXCR4, independent of the binding pocket (Asp171, Asp262, and Glu288) for CXCR4 inhibitor AMD3100, signifying that LL-37 is a distinct agonist of CXCR4. PMID: 30251699
2. Together, these data suggest that the S18-2 protein induces epithelial to mesenchymal cell transition through the TWIST2/E-cadherin signalling and, consequently, CXCR4-mediated migration of prostate cancer cells. PMID: 29396484
3. Study identified a variant near the chemokine receptor CXCR4 that was jointly associated with increased risk for progressive supranuclear palsy and Parkinson's disease. In addition, a mouse model of tauopathy, expression of CXCR4 and functionally associated genes was significantly altered in regions of the mouse brain that accumulate neurofibrillary tangles most robustly. PMID: 29636460
4. The expression of CXCR4 and mTOR were found to be negatively correlated with remission. Kaplan-Meier analysis indicated a significant decrease in the rate of progression-free survival (PFS) and in that of overall survival (OS) in patients positive for CXCR4 and mTOR expression. PMID: 28952842
5. the CXCL12-CXCR4 axis promotes the migration, invasion, and EMT processes in B-CPAP cells, at least partly, by activating the NF-kappaB signaling pathway. PMID: 29316404
6. results demonstrate that non-oxidizable HMGB1 induce a sustained cardiac fibroblasts migration despite the redox state of the environment and by altering CXCL12/CXCR4 axis. This affects proper cardiac remodeling after an infarction. PMID: 28716707
7. CXCR4 is highly abundant in the zona glomerulosa and in aldosterone producing adenomas suggesting a significant role in adrenocortical physiology and further representing a potential target for molecular imaging of aldosterone-producing tissue. PMID: 29279316
8. High CXCR4 expression is associated with bladder cancer progression. PMID: 30015971
9. The overexpression of CXCR4 increased sVCAM1, and the sVCAM1 secreted from CXCR4-overexpressing non-small cell lung carcinoma cells recruited and arrested additional osteoclast progenitors to promote osteoclastogenesis. PMID: 30355915
10. MiR-125b functions as an important downstream mediator upon the activation of CXCL12/CXCR4 axis. PMID: 28176874
11. Data suggest that CXCL12 and its receptor CXCR4 are critical in maintaining homeostasis, specifically during hematopoiesis. Present clinical trials (especially in hematological tumors) are testing whether adding CXCR4 inhibitors to impair tumor dissemination will increase effectiveness of ongoing anti-cancer treatments. (CXCL12 = C-X-C motif chemokine ligand 12; CXCR4 = C-X-C motif chemokine receptor-4) [REVIEW] PMID: 29288743
12. hypoxiainduced expression of CXCR4 promoted trophoblast cell migration and invasion via the activation of HIF1alpha, which is crucial during placentation. PMID: 29786753
13. CXCR4 expression was up-regulated in NSCLC cell lines. Inhibition of CXCR4 may reduce EMT, invasion and migration of NSCLC cells. PMID: 29972256
14. results suggest that BCP-ALL cells create a leukemic niche that attracts leukemic cells in a CXCR4/CXCL12-independent manner PMID: 28619846
15. Serum CXCR4 and CXCL12 levels increase significantly in septic neonates and they are valuable marker in diagnosis of neonatal sepsis. Serum concentrations of both chemokines represent promising novel biomarkers for neonatal sepsis. PMID: 28562124
16. study provides atomistic-level description of the activation dynamics of the C-X-C chemokine receptor type 4 (CXCR4), a class A GPCR and important drug target PMID: 30238101
17. CXCL12 and CXCR4 polymorphisms may be risk factors for hepatocellular carcinoma (HCC), and they may be potential HCC markers. PMID: 29741398
18. The results suggested that CXCR4 is a predictor of poor prognosis and may serve as a biomarker of the mesenchymal subtype in patients with Glioblastoma multiforme (GBM). In addition, CXCR4 mediated the mitogenactivated protein kinase signaling pathway, which was identified specifically in patients with mesenchymal GBM. PMID: 29767255
19. Stromal cell derived factor-1/C-X-C chemokine receptor type 4 axis induces human dental pulp stem cell migration through FAK/PI3K/Akt and GSK3beta/beta-catenin pathways. PMID: 28067275
20. EGFR Over-expression and Mutations Leading to Biological Characteristics Changes of Human Lung Adenocarcinoma Cells through CXCR4/CXCL12 Signaling Pathway PMID: 30037369
21. BACH1 may inhibit the progression of colorectal cancer through BACH1/CXCR4 pathway. PMID: 29481800
22. High CXCR4 expression is associated with differential expression patterns in adenocarcinoma and squamous cell carcinoma of the lung relative to small cell lung cancer. PMID: 30076481
23. No significant associations were found between mean plasma levels of either CXCL12 or CXCR4 with age, gender, tumor site, tumor size, lymph-node involvement or tumor stage PMID: 29693336
24. The aim of the present study was to assess whether fibrosis markers, estrogen receptor (ER)alpha and the stromal derived factor (SDF)1/CXC chemokine receptor type 4 (CXCR4) axis are abnormally expressed in Intrauterine adhesions endometrium. PMID: 29568895
25. Daily oral administration of AMD070 significantly inhibited the lung metastasis of B88SDF1 cells in nude mice. These results indicated that AMD070 could be useful as a novel orally bioavailable inhibitor of oral cancer metastasis PMID: 29749473
26. These data demonstrated that JWA suppressed the migration/invasion of breast carcinoma cells by downregulating the expression of CXCR4, and suggested that JWA may harbor prognostic and therapeutic potential in patients with breast cancer. PMID: 29658570
27. These results suggest that SDF1 (e.g. presented on proteoglycans) can rapidly activate integrins in an allosteric manner by binding to site 2 in the absence of CXCR4. The allosteric integrin activation by SDF1 is a novel target for drug discovery PMID: 29301984
28. High CXCR4 expression is associated with lymph node metastasis in colorectal cancer. PMID: 29719205
29. This effect can be suppressed by miR-613 through directly downregulating CXCR4. PMID: 29845707
30. These results suggest a key role for the CXCR4-CXCL12 chemokine axis in breast cancer progression and highlight the prognostic importance of this chemokine axis for breast cancer survival. PMID: 29516917
31. CXCR4 can induce PI3Kdelta inhibitor resistance in ABC DLBCL. PMID: 29472546
32. Our results demonstrated greater expression of pRET and CXCR4 in cisplatinresistant neuroblastomas (NBs). Vandetanib significantly inhibited SHSY5YR cell proliferation, colony formation, and invasion, while downregulating pRET and CXCR4 expression PMID: 29436676
33. disruption of the CXCR4/CXCL12 axis by CXCR4 antagonist AMD3100 blocked the contribution of both cancer and stromal cells to the metastatic cascade in the liver. PMID: 29436696
34. LncRNA PRNCR1 up-regulates CXCR4 through targeting miR-211-5p, which affects osteogenic differentiation and thus contributing to osteolysis after hip replacement PMID: 29775758
35. Results demonstrated that miR-1246 inhibited cell invasion and EMT process by targeting CXCR4 and blocking JAK/STAT and PI3K/AKT signal pathways in lung cancer cells. PMID: 29171984
36. High CXCR4 expression is associated with hepatocellular and cholangiocellular carcinomas in tumor capillaries. PMID: 29282035
37. Each of the CXCR4-derived peptides exhibited high affinity for GroEL with a binding stoichiometry near seven. It is found that the peptides interact with the paired alpha helices in the apical domain of the chaperonin. Each of the two chaperonin rings is competent for accommodating all the seven CXCR4 peptides bound to GroEL under saturation conditions. ATP alone or combined with GroES promoted the peptide release from... PMID: 29627450
38. Down-regulation of CXCR4 significantly reduced the cell proliferation, while remarkably increased the cell apoptosis and apoptotic protein expression levels in osteosarcoma cells. PMID: 29734183
39. Quercetin suppressed breast cancer stem cell proliferation, self-renewal, and invasiveness. It also lowered the expression levels of proteins related to tumorigenesis and cancer progression, such as aldehyde dehydrogenase 1A1, C-X-C chemokine receptor type 4, mucin 1, and epithelial cell adhesion molecules. PMID: 29353288
40. Icaritin enhances MSC proliferation, chemotaxis to stromal cell-derived factor-1 and osteogenic differentiation through STAT-3 activation, with a consequential up-regulation in the expression and activity of CXCR4. Phosphorylated STAT-3 binds the CXCr4 promoter upregulating its expression. PMID: 29679717
41. CXCL11 did not significantly alter the (13)C-(1)H-HSQC spectrum of CXCR4. Our findings point towards ubiquitin as a biased agonist of CXCR4 PMID: 28455789
42. High CXCR4 expression may define a specific subtype of sporadic malignant peripheral nerve sheath tumor with favorable prognosis. PMID: 29020982
43. Data support the importance of SDF-1 and CXCR4 expression for loco-regional control and overall survival in HNSCC after primary radiochemotherapy. PMID: 29061496
44. Presence of SST5, CXCR4 and ETA on tumor cells and of SST3, CXCR4 and ETA on microvessels gradually increased from grade II to grade IV tumors. PMID: 29696364
45. These data revealed that CXCR4 is a novel hepatocellular carcinoma (HCC)vascular marker for vessel sprouting and could serve as a potential therapeutic target and a predictive factor for sorafenib treatment in patients with HCC PMID: 28223275
46. Hetero-oligomerization of a1B/D-adrenergic receptor with the chemokine (C-X-C motif) receptor 4:atypical chemokine receptor 3 heteromeric complex is required for a1B/Dadrenergic receptor function. PMID: 28862946
47. CXCR4+ cells were increased in response to DOXO, mainly in human cardiac mesenchymal progenitor cells (CmPC), a subpopulation with regenerative potential. PMID: 28837147
48. This work demonstrates distinct roles for the SDF-1/CXCR4 or CXCR7 network in human induced pluripotent stem cell-derived ventricular cardiomyocyte specification, maturation and function. PMID: 28711757
49. implantation of IGF1R(+) human dental pulp mesenchymal stem cells exerted enhanced neuroplasticity via integrating inputs from both CXCR4 and IGF1R signaling pathways. PMID: 27586516
50. CXCR4 was overexpressed on systemic lupus erythematosus B cells, positively correlating with disease activity and kidney involvement PMID: 27665947
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相關疾病：

WHIM syndrome (WHIMS)
亞細胞定位：

Cell membrane; Multi-pass membrane protein. Cell junction. Early endosome. Late endosome. Lysosome. Note=In unstimulated cells, diffuse pattern on plasma membrane. On agonist stimulation, colocalizes with ITCH at the plasma membrane where it becomes ubiquitinated. In the presence of antigen, distributes to the immunological synapse forming at the T-cell-APC contact area, where it localizes at the peripheral and distal supramolecular activation cluster (SMAC).
蛋白家族：

G-protein coupled receptor 1 family
組織特異性：

Expressed in numerous tissues, such as peripheral blood leukocytes, spleen, thymus, spinal cord, heart, placenta, lung, liver, skeletal muscle, kidney, pancreas, cerebellum, cerebral cortex and medulla (in microglia as well as in astrocytes), brain microv
數據庫鏈接：

HGNC: 2561

OMIM: 162643

KEGG: hsa:7852

STRING: 9606.ENSP00000386884

UniGene: Hs.593413

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