Phospho-IRAK1 (T387) Antibody

說明書

中文名稱：

磷酸化-IRAK1 (T387)兔多克隆抗體
貨號：

CSB-PA011234
規格：

￥1090
其他：

產品詳情
產品評價
相關產品
靶點詳情

產品詳情

Uniprot No.：

P51617
基因名：

IRAK1
別名：

AA48924 antibody; Il1rak antibody; Interleukin 1 receptor associated kinase 1 antibody; Interleukin-1 receptor-associated kinase 1 antibody; IRAK antibody; IRAK-1 antibody; Irak1 antibody; IRAK1-S antibody; IRAK1_HUMAN antibody; mPLK antibody; OTTHUMP00000026014 antibody; OTTHUMP00000026015 antibody; OTTHUMP00000026020 antibody; OTTHUMP00000180621 antibody; Pelle antibody; Pelle homolog antibody; Pelle-like protein kinase antibody; Plpk antibody
宿主：

Rabbit
反應種屬：

Human,Mouse,Rat
免疫原：

Synthesized peptide derived from Human IRAK-1 around the phosphorylation site of T387.
免疫原種屬：

Homo sapiens (Human)
標記方式：

Non-conjugated
抗體亞型：

IgG
純化方式：

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
濃度：

It differs from different batches. Please contact us to confirm it.
保存緩沖液：

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
產品提供形式：

Liquid
應用范圍：

IF, ELISA

推薦稀釋比：

Application	Recommended Dilution
IF	1:200-1:1000
ELISA	1:5000

Protocols：

Immunofluorescence (IF) Protocol
ELISA Protocol
儲存條件：

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
貨期：

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
用途：

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產品評價

靶點詳情

功能：

Serine/threonine-protein kinase that plays a critical role in initiating innate immune response against foreign pathogens. Involved in Toll-like receptor (TLR) and IL-1R signaling pathways. Is rapidly recruited by MYD88 to the receptor-signaling complex upon TLR activation. Association with MYD88 leads to IRAK1 phosphorylation by IRAK4 and subsequent autophosphorylation and kinase activation. Phosphorylates E3 ubiquitin ligases Pellino proteins (PELI1, PELI2 and PELI3) to promote pellino-mediated polyubiquitination of IRAK1. Then, the ubiquitin-binding domain of IKBKG/NEMO binds to polyubiquitinated IRAK1 bringing together the IRAK1-MAP3K7/TAK1-TRAF6 complex and the NEMO-IKKA-IKKB complex. In turn, MAP3K7/TAK1 activates IKKs (CHUK/IKKA and IKBKB/IKKB) leading to NF-kappa-B nuclear translocation and activation. Alternatively, phosphorylates TIRAP to promote its ubiquitination and subsequent degradation. Phosphorylates the interferon regulatory factor 7 (IRF7) to induce its activation and translocation to the nucleus, resulting in transcriptional activation of type I IFN genes, which drive the cell in an antiviral state. When sumoylated, translocates to the nucleus and phosphorylates STAT3.
基因功能參考文獻：
1. The overexpression of miR-146a significantly increased the ErbB4 expression, decreased the expression of TRAF6, IRAK1, caspase 3, and the phosphorylation level of NF-kappaB, and also increased the Bcl-2/Bax ratio, suggesting the inhibition of inflammation and apoptosis. PMID: 30224945
2. our work extends the knowledge that IRAK1 is overexpressed and overactivated in CD4+ T cells from patients with SLE. The data highlight that when IRAK1 is blocked the differentiation of lupus naive CD4+ T cells into Th17 cells is repressed, suggesting therapeutic potential against SLE. PMID: 29611775
3. These collective data indicated that IRAK1 overexpression promotes endometrial carcinoma tumorigenesis by activating mitotic cell cycle and cell division pathways PMID: 28980703
4. Study identified IRAK1 and TRAF6 are direct targets of miR-146a in cervical cancer cells. Their expression is downregulated by miR-146a to promote cell viability. PMID: 29693168
5. The high mRNA levels of IRAK1 and IRAK4 were correlated with the development of Behcet's disease, which suggested that IRAK1 and IRAK4 might participate in the pathogenesis of Behcet's disease PMID: 28780618
6. MiRNA-146a rs2910164 and IRAK1 rs3027898 polymorphisms were a risk factor for predisposition to Rheumatoid Arthritis in Egyptian Population in codominant and dominant tested inheritance models, while, the miRNA-499 rs3746444 and PADI4 rs1748033 polymorphisms were a risk factor in codominant and recessive one. PMID: 29734142
7. Results show that the expression level of IRAK1 protein in papillary thyroid carcinoma (PTC) tissues was significantly decreased. Also, its expression level is regulated by miR-146a and miR-146b. PMID: 29048684
8. miR-146a suppresses the inflammatory response in human white adipocytes via targeting the expression of IRAK1 and TRAF6. PMID: 27922090
9. NLRC3 promoted K48-linked polyubiquitination and degradation of interleukin-1 receptor-associated kinase 1 (IRAK1). PMID: 28731142
10. miRNA146a expression was significantly higher in hepatitis C patients with a best cut off value 1.63 to discriminate between hepatitis C patients and healthy controls. Meanwhile, it was negatively correlated to IRAK1 and TRAF6 levels and positively correlated to viral load in hepatitis C patients. PMID: 28587864
11. These findings suggest that human IRAK-1 is essential downstream from TLRs but not IL-1Rs in fibroblasts, whereas it plays a redundant role downstream from both TLRs and IL-1Rs in leukocytes. PMID: 28069966
12. Expression of X-linked Toll-like receptor 4 signaling gene IRAK1 was significantly elevated in female neonates vs. male neonates. PMID: 28060792
13. The authors demonstrated that EV71 infection upregulates miR-21, which in turn suppresses EV71-triggered type I IFN production, thus promoting EV71 replication. Furthermore, they demonstrated that miR-21 targets the myeloid differentiation factor 88(MyD88) and interleukin-1 receptor-associated kinase 1(IRAK1), which are involved in EV71-induced type I IFN production. PMID: 28506791
14. Study shows that IRAK1 was over-expressed in human hepatocellular carcinoma (HCC) tumor tissues and provides evidence that IRAK1 promotes cell proliferation and protects against apoptosis in HCC. PMID: 27619757
15. These results suggest that the IRAK1-binding protein OPTN negatively regulates IL-1beta/LPS-induced NF-kappaB activation by preventing polyubiquitination of TRAF6. PMID: 28882891
16. IRAK1 regulates lipid accumulation by modulating CD36-mediated uptake and ABCA1-, ABCG1-dependent cholesterol efflux. PMID: 27270491
17. High IRAK1 expression is associated with multiple myeloma. PMID: 27454822
18. TLR4- and TLR2-induced IRAK-ERK pathway cross-talks with p67phox-Nox-2 for reactive oxygen species generation, thus regulating IL-1beta transcription and processing in monocytic cells. PMID: 26320741
19. Data suggest that, in monocytes and macrophages, the interleukin-1B- (IL1B)-stimulated trans-autophosphorylation of IRAK4 (interleukin-1 receptor-associated kinase 4) is initiated by MYD88- (myeloid differentiation primary response gene 88)-induced dimerization of IRAK4. In contrast, IRAK1 is inactive in unstimulated monocytes/macrophages and is converted to an active protein kinase in response to IL1B. PMID: 28512203
20. Data indicate the complexity of interactions between Pin1 and activated IRAK1, suggesting that phosphorylation of neighboring Ser/Thr-Pro motifs in proteins might provide competitive advantage at cellular concentrations for engaging with Pin1. PMID: 27790836
21. IRAK1 is a direct target of miR-146b and has functional roles to inhibit various aggressive papillary thyroid carcinoma cell activities. PMID: 27533309
22. our data strongly suggest that AQCA-mediated suppression of inflammatory responses could be managed by a direct interference of signaling cascades including IRAK and Syk, linked to the activation of NF-kappaB and AP-1. PMID: 27338330
23. The IRAK1 rs3027898 was not associated with RA, whereas C allele of miR-146a rs2910164 was found to be protective. PMID: 28207326
24. the Xq28 region containing TMEM187 and IRAK1 (rs13397, rs1059703, and rs1059702) with rheumatoid arthritis (RA)susceptibility; results replicated association of the 3 Xq28 polymorphisms with RA risk in Tunisian and French populations and suggested that RA susceptibility is associated with TMEM187-IRAK1 polymorphisms in women PMID: 28271077
25. IRAK1 expression was not significantly increased in tympanic membrane of otitis media patients. PMID: 27497395
26. Our findings reveal that IRAK1 promotes cell survival and is an attractive therapeutic target in head and neck squamous cell carcinomas PMID: 26527316
27. miR146a has an important promoting effect on the apoptosis of granulosa cells by targeting IRAK1 and TRAF6 via the caspase cascade pathway PMID: 26151128
28. This is the first study to show an association between single nucleotide polymorphisms in IRAK1, IRAK4 and MyD88, and the presence of severe invasive pneumococcal disease. PMID: 26075815
29. This meta-analysis suggests that the miR-499 rs374644 and IRAKI rs3027898 polymorphisms are associated with susceptibility to inflammatory arthritis. [review] PMID: 25269878
30. IRAK1 overexpression drive aggressive growth, metastasis and acquired resistance to paclitaxel treatment in breast cancer. PMID: 26503059
31. Data suggest that TLR2 (toll like receptor 2) is down-regulated by microRNA-UL112-3p from human Cytomegalovirus; microRNA-UL112-3p also down-regulates TLR2-induced post-translational activation of IRAK1 signaling. PMID: 25955717
32. These data demonstrated that these three single nucleotide polymorphisms (rs3027898, rs1059702, rs1059703) in IRAK1 were associated with autoimmune diseases risk. PMID: 26142671
33. Src, Syk, IRAK1, and IRAK4 have roles in anti-inflammatory responses mediated by dietary flavonoid Kaempferol PMID: 25922567
34. High mRNA levels of IRAK1 and IRAK4 correlated with VKH disease activity. PMID: 24690905
35. expression of IRAK1 in lung cancer was significantly higher compared with that in normal lung tissues and was correlated with TNM stage, lymphatic metastasis and tumor size. PMID: 25550857
36. results firstly indicated that IRAK1 and MECP2 genes are crucial risk factors for AITDs. PMID: 25458699
37. IRAK1 was constitutively phosphorylated in PEL and required for survival, implicating IRAK1 and TLR signaling as a driver pathway in PEL PMID: 25341731
38. MicroRNA-146a and microRNA-146b regulate human dendritic cell apoptosis and cytokine production by targeting TRAF6 and IRAK1 proteins. PMID: 25505246
39. IL-1beta stimulation causes sequential phosphorylation of IRAK-1, c-Jun N-terminal kinase, and p300 and enhances recruitment of the p300/CBP/NF-Y complex to Gankyrin promoter PMID: 25294684
40. The IRAK1 polymorphism is a strong independent predictor of multiple organ failure and mortality postinjury and represents a common variant with prognostic potential. PMID: 25203887
41. Significantly lower levels of IRAK-1 was found in CAD patients with the CC genotype. PMID: 23794009
42. miR-146a enhances the oncogenicity of oral carcinoma by concomitant targeting of the IRAK1, TRAF6 and NUMB genes. PMID: 24302991
43. A direct interaction between IRAK1 and vasodilator-stimulated phosphoprotein (VASP) is regulated in part by assembly of IRAK1. PMID: 24857403
44. IRAK1 rs1059702 genetic variant do not play a significant role in giant cell arteritis susceptibility or severity. PMID: 24709033
45. Data indicate that acetyl-11-keto-beta-boswellic acid (AKBA) is able to decrease Th17 differentiation by inhibition of IL-1beta signaling via reduction of IL-1 receptor-associated kinase 1 (IRAK1) phosphorylation. PMID: 24469975
46. Female very low birth weight infants heterozygous for the X-linked IRAK1 (rs1059703) SNP had lessgram negative bacterial infections. PMID: 23867959
47. The data suggest the existence of two independent signals within the Xq28 region, one in IRAK1 related to pulmonary fibrosis and another in MECP2 related to diffuse cutaneous systemic sclerosis. PMID: 23444193
48. results indicate that miR-21 is upregulated during hepatitis C virus infection and negatively regulates IFN-alpha signaling through MyD88 and IRAK1 PMID: 23633945
49. The polymorphisms rs3027898 and rs1059702 of IRAK1 gene are associated with systemic lupus erythematosus in the Chinese Han population. PMID: 23435933
50. Data suggest that the lupus-associated variant in the MECP2/IRAK1 locus has the potential to affect all 3 epigenetic mechanisms: DNA methylation, microRNA expression, and histone modification. PMID: 23428850
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亞細胞定位：

Cytoplasm. Nucleus. Lipid droplet.
蛋白家族：

Protein kinase superfamily, TKL Ser/Thr protein kinase family, Pelle subfamily
組織特異性：

Isoform 1 and isoform 2 are ubiquitously expressed in all tissues examined, with isoform 1 being more strongly expressed than isoform 2.
數據庫鏈接：

HGNC: 6112

OMIM: 300283

KEGG: hsa:3654

STRING: 9606.ENSP00000358997

UniGene: Hs.522819

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