PIH1D1 Antibody

說(shuō)明書(shū)

中文名稱：

PIH1D1兔多克隆抗體
貨號(hào)：

CSB-PA889130LA01HU
規(guī)格：

￥440
圖片：
Immunohistochemistry of paraffin-embedded human placenta tissue using CSB-PA889130LA01HU at dilution of 1:100

Immunofluorescence staining of Hela cells cells with CSB-PA889130LA01HU at 1: 200, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized tissue using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C.The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L) .

Immunoprecipitating PIH1D1 in Hela whole cell lysate
Lane 1: Rabbit control IgG (1ug) instead of CSB-PA889130LA01HU in Hela whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000)
Lane 2: CSB-PA889130LA01HU (6ug) + Hela whole cell lysate (500ug)
Lane 3: Hela whole cell lysate (10ug)

Western Blot
Positive WB detected in: Hela whole cell lysate, A549 whole cell lysate, U87 whole cell lysate, K562 whole cell lysate, 293 whole cell lysate
All lanes: PIH1D1 antibody at 1:2000
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 33, 20, 22 kDa
Observed band size: 39 kDa
其他：

產(chǎn)品詳情
產(chǎn)品評(píng)價(jià)
相關(guān)產(chǎn)品
靶點(diǎn)詳情

產(chǎn)品詳情

產(chǎn)品名稱：

Rabbit anti-Homo sapiens (Human) PIH1D1 Polyclonal antibody
Uniprot No.：

Q9NWS0
基因名：

PIH1D1
別名：

NOP17 antibody; Nucleolar protein 17 homolog antibody; PIH1 domain containing 1 antibody; PIH1 domain containing protein 1 antibody; PIH1 domain-containing protein 1 antibody; PIH1D1 antibody; PIHD1_HUMAN antibody
宿主：

Rabbit
反應(yīng)種屬：

Human
免疫原：

Recombinant Human PIH1 domain-containing protein 1 protein (1-290AA)
免疫原種屬：

Homo sapiens (Human)

標(biāo)記方式：

Non-conjugated

本頁(yè)面中的產(chǎn)品，PIH1D1 Antibody (CSB-PA889130LA01HU)，的標(biāo)記方式是Non-conjugated。對(duì)于PIH1D1 Antibody，我們還提供其他標(biāo)記。見(jiàn)下表：

標(biāo)記方式	貨號(hào)	產(chǎn)品名稱	應(yīng)用
HRP	CSB-PA889130LB01HU	PIH1D1 Antibody, HRP conjugated	ELISA
FITC	CSB-PA889130LC01HU	PIH1D1 Antibody, FITC conjugated
Biotin	CSB-PA889130LD01HU	PIH1D1 Antibody, Biotin conjugated	ELISA

克隆類型：

Polyclonal
抗體亞型：

IgG
純化方式：

>95%, Protein G purified
濃度：

It differs from different batches. Please contact us to confirm it.
保存緩沖液：

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
產(chǎn)品提供形式：

Liquid
應(yīng)用范圍：

ELISA, WB, IHC, IF, IP

推薦稀釋比：

Application	Recommended Dilution
WB	1:500-1:5000
IHC	1:20-1:200
IF	1:50-1:500
IP	1:200-1:2000

Protocols：

ELISA Protocol
Western Blotting (WB) Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol
Immunoprecipitation (IP) Protocol
儲(chǔ)存條件：

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
貨期：

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
用途：

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

功能：

Involved in the assembly of C/D box small nucleolar ribonucleoprotein (snoRNP) particles. Recruits the SWI/SNF complex to the core promoter of rRNA genes and enhances pre-rRNA transcription. Mediates interaction of TELO2 with the R2TP complex which is necessary for the stability of MTOR and SMG1. Positively regulates the assembly and activity of the mTORC1 complex.
基因功能參考文獻(xiàn)：
1. We propose that protein complexes assembled by the R2TP complex are defined by phosphorylation of a specific motif and recognition by the PIH1D1 subunit PMID: 24656813
2. PIH1D1 interacts with mTOR complex 1 and enhances ribosome RNA transcription. PMID: 24036451
3. Human PIH1 domain-containing protein 1 (PIH1) interacts directly with histone H4 and recruits the Brg1-SWI/SNF complex via SNF5 to human rRNA genes. PMID: 22368283
4. this study investigated the interaction between RPAP3 and PIH1D1. PMID: 23159623
5. PIH1D1 may stabilize SNF5 by attenuating its proteasome degradation pathway. PMID: 20078948
6. These results suggest that PIH1D1 may also function as a novel modulator of apoptosis pathway. PMID: 21078300
7. Is part of an RNA polymerase II-associated complex with possible chaperone activity. PMID: 19450687
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亞細(xì)胞定位：

Nucleus.
蛋白家族：

PIH1 family
組織特異性：

Expressed at low levels in normal mammary epithelial cells (at protein level). Highest expression in lung, leukocyte and placenta. Expressed at lower levels in brain, prostate, colon, heart, small intestine, liver, ovary, pancreas, skeletal muscle, spleen
數(shù)據(jù)庫(kù)鏈接：

HGNC: 26075

OMIM: 611480

KEGG: hsa:55011

STRING: 9606.ENSP00000262265

UniGene: Hs.5245

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