HACE1 Antibody

中文名稱：

HACE1兔多克隆抗體
貨號：

CSB-PA818264LA01HU
規(guī)格：

￥440
圖片：
IHC image of CSB-PA818264LA01HU diluted at 1:600 and staining in paraffin-embedded human heart tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

IHC image of CSB-PA818264LA01HU diluted at 1:600 and staining in paraffin-embedded human brain tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
其他：

產(chǎn)品詳情
產(chǎn)品評價
相關(guān)產(chǎn)品
靶點詳情

產(chǎn)品詳情

產(chǎn)品名稱：

Rabbit anti-Homo sapiens (Human) HACE1 Polyclonal antibody
Uniprot No.：

Q8IYU2
基因名：

HACE1
別名：

HACE1 antibody; KIAA1320E3 ubiquitin-protein ligase HACE1 antibody; EC 2.3.2.26 antibody; HECT domain and ankyrin repeat-containing E3 ubiquitin-protein ligase 1 antibody; HECT-type E3 ubiquitin transferase HACE1 antibody
宿主：

Rabbit
反應(yīng)種屬：

Human
免疫原：

Recombinant Human E3 ubiquitin-protein ligase HACE1 protein (333-442AA)
免疫原種屬：

Homo sapiens (Human)

標(biāo)記方式：

Non-conjugated

本頁面中的產(chǎn)品，HACE1 Antibody (CSB-PA818264LA01HU)，的標(biāo)記方式是Non-conjugated。對于HACE1 Antibody，我們還提供其他標(biāo)記。見下表：

標(biāo)記方式	貨號	產(chǎn)品名稱	應(yīng)用
HRP	CSB-PA818264LB01HU	HACE1 Antibody, HRP conjugated	ELISA
FITC	CSB-PA818264LC01HU	HACE1 Antibody, FITC conjugated
Biotin	CSB-PA818264LD01HU	HACE1 Antibody, Biotin conjugated	ELISA

克隆類型：

Polyclonal
抗體亞型：

IgG
純化方式：

>95%, Protein G purified
濃度：

It differs from different batches. Please contact us to confirm it.
保存緩沖液：

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
產(chǎn)品提供形式：

Liquid
應(yīng)用范圍：

ELISA, IHC

推薦稀釋比：

Application	Recommended Dilution
IHC	1:500-1:1000

Protocols：

ELISA Protocol
Immunohistochemistry (IHC) Protocol
儲存條件：

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
貨期：

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
用途：

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

產(chǎn)品評價

靶點詳情

功能：

E3 ubiquitin-protein ligase involved in Golgi membrane fusion and regulation of small GTPases. Acts as a regulator of Golgi membrane dynamics during the cell cycle: recruited to Golgi membrane by Rab proteins and regulates postmitotic Golgi membrane fusion. Acts by mediating ubiquitination during mitotic Golgi disassembly, ubiquitination serving as a signal for Golgi reassembly later, after cell division. Specifically interacts with GTP-bound RAC1, mediating ubiquitination and subsequent degradation of active RAC1, thereby playing a role in host defense against pathogens. May also act as a transcription regulator via its interaction with RARB.
基因功能參考文獻(xiàn)：
1. A functional interplay between HACE1 and Rac1 in cancer, is reported. PMID: 28317937
2. Results identified HACE1 Ser-385 as a pivotal amino acid residue that is phosphorylated in response to CNF1 or VEGF treatments and that expression of activated Rac1 is sufficient to induce HACE1 phosphorylation and controlling its oligomerization state. PMID: 29362425
3. Results indicated that HACE1 rs9404576 polymorphism may be associated with Wilms' tumor susceptibility in the Chinese population. PMID: 29243987
4. 3-deazaneplanocin A treatment increased HACE1 gene expression which was further increased by the addition of trichostatine A (TSA), a promising therapeutic compound for the treatment of human B-Lymphoma PMID: 28651105
5. HACE1 can act as a haploinsufficient tumor suppressor gene in most B-cell lymphomas and can be downregulated by deacetylation of its promoter region chromatin PMID: 27107267
6. Hepatocellular carcinoma patients with low HACE1 expression levels exhibited poorer overall survival and HACE1 was found to be an independent prognostic factor for survival. PMID: 27805249
7. HACE1 as a specific E3 ligase that polyubiquitinates YB-1 through non-canonical K27 linked ubiquitin chains. PMID: 26343856
8. Biallelic loss-of-function mutations in HACE1 are associated with an autosomal recessive neurodevelopmental syndrome. PMID: 26424145
9. supports a critical role for HACE1 in breast cancer progression and identifies patients that may benefit from Rac-targeted therapies PMID: 25659579
10. contrary to previous report, overexpression of HACE1 by transduction of recombinant protein did not affect proliferation or survival of NKTCL cell lines. We therefore conclude that HACE1 is not directly involved in NKTCL pathophysiology PMID: 25203537
11. HACE1-OPTN axis synergistically suppresses growth and tumorigenicity of lung cancer cells. PMID: 25026213
12. Ubiquitylation and activation of RAB11a is promoted by a beta2 adrenergic receptor-HACE1 complex. PMID: 24190883
13. HACE1 reduces oxidative stress and mutant Huntingtin toxicity by promoting the NRF2 response. PMID: 24516159
14. All stages of HCCs presented HACE1 methylation, indicating that the HACE1 gene has been methylated from the early stages of HCCs. PMID: 23732777
15. HACE1 is tumor suppressor gene located within the 6q21 region, and loss of function of multiple tumor suppressor genes within 6q21 may be a critical determinant of NK cell lymphomagenesis. PMID: 23142381
16. HACE1 is an antagonist of cell migration through its ability to degrade active Rac1. PMID: 22614015
17. common variants in HACE1 and LIN28B influence neuroblastoma susceptibility and indicate that both genes likely have a role in disease progression. PMID: 22941191
18. The HACE1 expression increases the ubiquitylation of Rac1, when the GTPase is activated by point mutations or by the GEF-domain of Dbl. PMID: 22036506
19. The 6q21-22 region was confirmed as a celiac disease susceptibility locus and harbors multiple independent associations, some of which may implicate ubiquitin-pathways in celiac disease susceptibility, such as mutations in gene HACE1. PMID: 21326284
20. Deregulation of the tumor suppressor HACE1 is associated with natural killer/T-cell lymphoma, nasal type. PMID: 19965620
21. Hace1 as a novel ubiquitin-protein ligase with very low expression Wilms' tumors; hypermethylation of two upstream CpG islands correlates with low HACE1 expression in tumor samples. PMID: 15254018
22. HACE1 is a candidate chromosome 6q21 tumor-suppressor gene involved in multiple cancers. PMID: 17694067
23. Aberrant methylation of the HACE1 gene was detected in 9 out of the 32 (28%) primary colon carcinomas. PMID: 18630515
24. Aberrant methylation of the HACE1 gene is associated with gastric carcinoma. PMID: 19528486
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相關(guān)疾病：

Spastic paraplegia and psychomotor retardation with or without seizures (SPPRS)
亞細(xì)胞定位：

Golgi apparatus, Golgi stack membrane. Cytoplasm. Endoplasmic reticulum. Note=A significant portion localizes to the endoplasmic reticulum. Targeted to Golgi membrane via its interaction with Rab proteins.
組織特異性：

Expressed in multiple tissues including heart, brain and kidney.
數(shù)據(jù)庫鏈接：

HGNC: 21033

OMIM: 610876

KEGG: hsa:57531

STRING: 9606.ENSP00000262903

UniGene: Hs.434340

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